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公开(公告)号：US10196644B2
公开(公告)日：2019-02-05
申请号：US15317939
申请日：2014-06-11
Inventor： Sang Yup Lee , Minho Roh , Seung Min Yoo
IPC: C12N15/67 , C12N15/113
Abstract: The present invention relates to a method for fine-tuning gene expression levels using a synthetic regulatory sRNA in a prokaryotic cell. The present invention can simultaneously, easily, and quickly apply various target gene combinations to various strains without gene deletion through the synthetic regulatory sRNA for regulating gene expression and is therefore very suitable for measuring the metabolizability of each strain and selecting an optimum strain. In addition, the method has the advantages of easily and quickly selecting a target gene for the inhibition of gene expression and expressing the gene thus selected to a desired degree and thus can be used in producing recombinant strains for the efficient production of various metabolites and establishing a method for the efficient production and is therefore very useful.
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公开(公告)号：US20190024093A1
公开(公告)日：2019-01-24
申请号：US16071680
申请日：2016-12-13
Inventor： Sang Yup Lee , So Young Choi , Si Jae Park
Abstract: The present invention relates to a recombinant microorganism having the ability to produce poly(lactate-co-glycolate) and its copolymers from xylose, and more particularly to a recombinant microorganism having the ability to produce poly(lactate-co-glycolate) and its copolymers without having to supply a glycolate precursor from an external source, and a method of producing a poly(lactate-co-glycolate) copolymers using the same.
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13.发明申请公开(公告)号：US20180051291A1
公开(公告)日：2018-02-22
申请号：US15556458
申请日：2016-03-17
Inventor： Sang Yup Lee , Tong Un Chae , Chan Woo Song
CPC classification number: C12N15/52 , C12N1/20 , C12N9/88 , C12N15/01 , C12N15/63 , C12P7/40 , C12P7/42 , C12P13/001 , C12P13/005 , C12P13/04 , C12P13/06 , C12R1/00
Abstract: The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.
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公开(公告)号：US11542483B2
公开(公告)日：2023-01-03
申请号：US17366269
申请日：2021-07-02
Inventor： Sang Yup Lee , Junho Bang , Chang Hun Hwang , Jung Ho Ahn , Jong An Lee
IPC: C12P7/46 , C12N9/10 , C12P7/02 , C12N9/04 , C12N9/00 , C12N9/78 , C12P7/16 , C12P7/04 , C12P13/10 , C12P5/00 , C12P5/02 , C12P13/00 , C12P7/42 , C12P17/10 , C12P13/06 , C12P13/08
Abstract: Disclosed is a recombinant microorganism capable of growing using only carbon dioxide and formic acid by introducing and improving a metabolic pathway for synthesizing pyruvic acid from carbon dioxide and formic acid to enhance pyruvic acid synthesis efficiency and performing additional genetic manipulation, and a method for producing useful substances using the same. Advantageously, the recombinant microorganism is capable of synthesizing pyruvic acid, a C3 organic compound, at a remarkably improved rate, and in particular, grows well even in a medium containing only carbon dioxide and formic acid as carbon sources without a glucose supply, and is thereby capable of synthesizing pyruvic acid and various high value-added compounds using the same as an intermediate product in an economically efficient manner.
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15.发明申请公开(公告)号：US20190024124A1
公开(公告)日：2019-01-24
申请号：US16068475
申请日：2016-12-29
Inventor： Sang Yup Lee , Yoojin Choi
IPC: C12P3/00 , C07K14/825 , C12N9/10
Abstract: The present invention relates to a method of producing metal nanoparticles and metal sulfide nanoparticles using a recombinant microorganism co-expressing metallothionein and phytochelatin synthase, which are heavy metal-adsorbing proteins, and to the use of metal nanoparticles and metal sulfide nanoparticles synthesized by the method. The present invention provides a method for synthesizing metal nanoparticles which have been difficult to synthesize by conventional biological methods. The present invention makes it possible to synthesize metal nanoparticles in an environmentally friendly and cost-effective manner, and also makes it possible to synthesize metal sulfide nanoparticles. In addition, even metal nanoparticles which could have been produced by conventional chemical or biological methods are produced in a significantly increased yield by use of the method of the present invention.
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Patent Agency Ranking
公开(公告)号：US20170130235A1
公开(公告)日：2017-05-11
申请号：US15317939
申请日：2014-06-11
Inventor： Sang Yup Lee , Minho Roh , Seung Min Yoo
IPC: C12N15/67 , C12N15/113
CPC classification number: C12N15/67 , C12N15/113 , C12N2310/11 , C12N2310/111 , C12N2310/531
Abstract: The present invention relates to a method for fine-tuning gene expression levels using a synthetic regulatory sRNA in a prokaryotic cell. The present invention can simultaneously, easily, and quickly apply various target gene combinations to various strains without gene deletion through the synthetic regulatory sRNA for regulating gene expression and is therefore very suitable for measuring the metabolizability of each strain and selecting an optimum strain. In addition, the method has the advantages of easily and quickly selecting a target gene for the inhibition of gene expression and expressing the gene thus selected to a desired degree and thus can be used in producing recombinant strains for the efficient production of various metabolites and establishing a method for the efficient production and is therefore very useful.
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17.发明授权公开(公告)号：US09428774B2
公开(公告)日：2016-08-30
申请号：US14874385
申请日：2015-10-03
Inventor： Sang Yup Lee , Sung Won Lim , Hyohak Song
CPC classification number: C12P7/46 , C12N1/20 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N15/902 , C12Y203/01054 , Y02P20/52
Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.
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公开(公告)号：US11655462B2
公开(公告)日：2023-05-23
申请号：US16490515
申请日：2019-05-02
Inventor： Sang Yup Lee , Kyung-Jin Kim , Jung Ho Ahn , Hogyun Seo , Jong An Lee
CPC classification number: C12N9/0006 , C12N1/20 , C12N15/74 , C12P7/46 , C12Y101/01037
Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.
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公开(公告)号：US11473111B2
公开(公告)日：2022-10-18
申请号：US17055971
申请日：2019-04-24
Inventor： Sang Yup Lee , Jae Sung Cho , Je Woong Kim , Cindy Pricilia Surya Prabowo , Taehee Han , Yoo Sung Ko
Abstract: The present invention relates to recombinant Corynebacterium having 1,3-PDO productivity and reduced 3-HP productivity, and a method for producing 1,3-PDO by using same. When a Corynebacterium glutamicum variant according to the present invention is used, the productivity of 3-HP, which is a by-product, is inhibited by using low-cost glycerol as a carbon source, and thus 1,3-PDO can be produced with high efficiency.
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公开(公告)号：US11214816B2
公开(公告)日：2022-01-04
申请号：US16472876
申请日：2017-11-20
Inventor： Sang Yup Lee , Junho Bang
Abstract: The present invention relates to a recombinant microorganism having heterologous genes introduced thereto and a method for producing a useful material from formic acid and carbon dioxide using the microorganism. The present invention provides a novel microorganism having a cyclic metabolic pathway introduced thereto through which C3 or higher carbon organic compounds can be synthesized from formic acid and carbon dioxide, whereby carbon dioxide rich in nature and formic acid that is of low toxicity and suitable for anabolic reaction in view of reaction kinetics and which can be easily and rapidly synthesized from carbon dioxide can be used to effectively synthesize the C3 organic compound pyruvic acid from which various high-value added compound can be synthesized.
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