利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

38 条记录 (耗时 0.059 秒)

    Method for identification and isolation of DNA encoding a desired protein
    14.
    发明授权
    Method for identification and isolation of DNA encoding a desired protein 失效
    用于鉴定和分离编码所需蛋白质的DNA的方法

    公开(公告)号：US4675285A

    公开(公告)日：1987-06-23

    申请号：US652316

    申请日：1984-09-19

    申请人： Steven C. Clark ,  Randal J. Kaufman ,  Gordon G. Wong

    发明人： Steven C. Clark ,  Randal J. Kaufman ,  Gordon G. Wong

    IPC分类号： C07K14/53 ,  C12N15/10 ,  C12N15/85 ,  C12Q1/68 ,  C12N5/00 ,  C12N15/00 ,  C12P21/00

    CPC分类号： C12N15/1086 ,  C07K14/53 ,  C12N15/85

    摘要： A method for identifying and isolating clones containing DNA coding for a desired protein is described. DNA prepared from a cell that expresses the desired protein is inserted into an isolation expression vector having means for replication (as a means of producing DNA) and a suitable promoter for expression of said DNA in a predetermined mammalian host cell as well as means for replication in a bacterial cell. The transient expression vector is then inserted into a bacterial cell for replication of the DNA. Pools of DNA, prepared from a predetermined number of bacterial clones so that the nucleic acids (DNA and RNA) is substantially free of other bacterial contaminants are transfected or microinjected into mammalian host cells and conditioned medium from growing such cells is tested for the presence of the desired protein. Positive pools are selected and the clones used to make the pool are screened to identify and isolate the clone containing the desired DNA.

    摘要翻译： 描述了鉴定和分离含有编码所需蛋白质的DNA的克隆的方法。 将从表达所需蛋白质的细胞制备的DNA插入具有复制方式的分离表达载体（作为生产DNA的手段）和用于在预定哺乳动物宿主细胞中表达所述DNA的合适启动子以及复制手段 在细菌细胞中。 然后将瞬时表达载体插入用于DNA复制的细菌细胞中。 从预定数量的细菌克隆制备DNA，使得核酸（DNA和RNA）基本上不含其它细菌污染物的DNA转染或显微注射到哺乳动物宿主细胞中，并测试来自培养这样的细胞的条件培养基的存在 所需蛋白质。 选择阳性池，筛选用于制备池的克隆以鉴定并分离含有所需DNA的克隆。

    Methods of identifying nucleic acid probes to quantify the expression of a target nucleic acid
    17.
    发明授权
    Methods of identifying nucleic acid probes to quantify the expression of a target nucleic acid 有权
    鉴定核酸探针以量化靶核酸的表达的方法

    公开(公告)号：US06548257B2

    公开(公告)日：2003-04-15

    申请号：US09935365

    申请日：2001-08-22

    申请人： David J. Lockhart ,  Eugene L. Brown ,  Gordon G. Wong ,  Mark Chee ,  Thomas R. Gingeras

    发明人： David J. Lockhart ,  Eugene L. Brown ,  Gordon G. Wong ,  Mark Chee ,  Thomas R. Gingeras

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6809 ,  C12Q1/6837 ,  G01N15/1475 ,  C12Q2565/507

    摘要： This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

    摘要翻译： 本发明提供监测多种基因的表达水平的方法。 所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。 在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中 包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸被定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸探针与RNA转录物互补 衍生自RNA转录物的核酸; 并定量阵列中的杂交核酸。

    Recombinant DNA endoding an erythropoietin receptor
    20.
    发明授权
    Recombinant DNA endoding an erythropoietin receptor 失效
    重组DNA代替红细胞生成素受体

    公开(公告)号：US5278065A

    公开(公告)日：1994-01-11

    申请号：US678877

    申请日：1991-03-25

    申请人： Alan D'Andrea ,  Gordon G. Wong ,  Simon S. Jones

    发明人： Alan D'Andrea ,  Gordon G. Wong ,  Simon S. Jones

    IPC分类号： A61K38/00 ,  C07K14/505 ,  C07K14/71 ,  C07K16/22 ,  C12N15/12 ,  C12P21/08 ,  C12N15/63

    CPC分类号： C07K16/22 ,  C07K14/505 ,  C07K14/71 ,  A61K38/00

    摘要： The invention described encompasses an isolated DNA sequence encoding all or a portion thereof of a cell surface receptor for murine and human erythropoietin (hereinafter EPO-R), along with the isolated polypeptide expressed by the DNA sequence (i.e., isolated EPO-R). The invention also encompasses host cells containing the above-described DNA sequence, preferably, host cells which express the polypeptide encoded by the DNA sequence (EPO-R) at a significantly higher level than that produced by normal red blood cell precursors. The invention further encompasses DNA sequences encoding secreted forms of the human EPO-R and polypeptides corresponding thereto. The EPO-receptor in all of the disclosed forms can be used as models for designing drugs or in pharmaceutical compositions for treating anemias.

    摘要翻译： 描述的发明包括编码鼠和人促红细胞生成素（以下称为EPO-R）的细胞表面受体的全部或一部分的分离的DNA序列，以及由DNA序列表达的分离的多肽（即分离的EPO-R）。 本发明还包括含有上述DNA序列的宿主细胞，优选表达由DNA序列（EPO-R）编码的多肽的宿主细胞，其水平高于由正常红细胞前体产生的水平。 本发明还包括编码人EPO-R分泌形式的DNA序列和与其对应的多肽。 所有公开形式的EPO-受体可用作设计药物的模型或用于治疗贫血的药物组合物。