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32 条记录 (耗时 0.016 秒)

    High alkaline protease and use thereof
    12.
    发明授权
    High alkaline protease and use thereof 失效
    高碱性蛋白酶及其用途

    公开(公告)号：US08153413B2

    公开(公告)日：2012-04-10

    申请号：US11719713

    申请日：2005-11-16

    申请人： Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

    发明人： Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

    IPC分类号： C12N9/00 ,  C12N9/92 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/52

    摘要： The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis. The protease cleaves 26 peptide bonds among 29 peptide bonds of acidic insulin B-chain.

    摘要翻译： 本发明的目的在于提供一种具有高碱性，耐表面活性和钙依赖性热稳定性等特性，在高碱性洗涤剂中表现出优异性能的新型碱性蛋白酶及其氨基酸序列的编码基因。 提供了具有这样的性质的碱性蛋白酶，其活性pH范围为5至13，最佳pH为约12.6，最适温度为70℃，在65℃下不观察到加热下的活性降低 pH 10，最佳温度和热稳定性不受Ca2 +离子的影响。 具体地，例如提供了具有SEQ ID NO：3所示的构成成熟酶的氨基酸序列的氨基酸序列的碱性蛋白酶或由缺失，取代，位置反转排列，添加或插入部分 的氨基酸，或衍生自变形白菜（Alkaliphillus transvaalensis）。 蛋白酶在酸性胰岛素B链的29个肽键中切割26个肽键。

    Maltose phosohorylase, trehalose phosphorylase, Plesiomonas strain and preparation process of trehalose
    15.
    发明授权
    Maltose phosohorylase, trehalose phosphorylase, Plesiomonas strain and preparation process of trehalose 失效
    麦芽糖磷酸化酶，海藻糖磷酸化酶，细菌菌株和海藻糖的制备方法

    公开(公告)号：US5807719A

    公开(公告)日：1998-09-15

    申请号：US893504

    申请日：1997-07-11

    申请人： Masahiro Yoshida ,  Nobuyuki Nakamura ,  Koki Horikoshi

    发明人： Masahiro Yoshida ,  Nobuyuki Nakamura ,  Koki Horikoshi

    IPC分类号： C12N9/10 ,  C12P19/12 ,  C12N9/12 ,  C12N9/14 ,  C12N9/24

    CPC分类号： C12N9/1051 ,  C12P19/12 ,  C12R1/01

    摘要： Disclosed are a novel microorganism (FERM BP-5144) belonging to the genus Plesiomonas and having ability to produce maltose phosphorylase and trehalose phosphorylase required for the enzymatic production of trehalose and novel maltose phosphorylase and trehalose phosphorylase obtainable from the microorganism as well as a process for producing the enzymes. A novel process for enzymatically producing trehalose (O-.alpha.-D-glucopyranosyl-(1.fwdarw.1)-D-glucopyranoside) is also disclosed.

    摘要翻译： 本发明公开了一种属于扁球菌属的新型微生物（FERM BP-5144），具有产生可从微生物获得的海藻糖和新型麦芽糖磷酸化酶和海藻糖磷酸化酶所需的麦芽糖磷酸化酶和海藻糖磷酸化酶所需的能力， 产生酶。 还公开了一种用于酶促生产海藻糖（O-α-D-吡喃葡萄糖基 - （1-> 1）-D-吡喃葡萄糖苷）的新方法。

    Process for the oil extraction from oil sand by using cyclodextrin
    17.
    发明授权
    Process for the oil extraction from oil sand by using cyclodextrin 失效
    通过使用环糊精从油砂中提取油的工艺

    公开(公告)号：US4444647A

    公开(公告)日：1984-04-24

    申请号：US340501

    申请日：1982-01-18

    申请人： Koki Horikoshi ,  Ichiro Shibanai

    发明人： Koki Horikoshi ,  Ichiro Shibanai

    IPC分类号： C10G1/00 ,  C10G1/04

    CPC分类号： C10G1/047

    摘要： Process for the oil extraction from oil sand comprising: mixing oil sand, cyclodextrin and water with one another to prepare a suspension, leaving it to stand or centrifuging it to separate into an oil, a water and a sand layers, and then collecting the oil layer.

    摘要翻译： 从油砂提取油的方法包括：将油砂，环糊精和水彼此混合以制备悬浮液，使其静置或离心分离成油，水和砂层，然后收集油 层。

    Solid cellulosic culture medium and process for producing the same
    18.
    发明授权
    Solid cellulosic culture medium and process for producing the same 有权
    固体纤维素培养基及其制备方法

    公开(公告)号：US07790457B2

    公开(公告)日：2010-09-07

    申请号：US10591288

    申请日：2005-02-28

    申请人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

    发明人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

    IPC分类号： C12N5/00 ,  A61K9/00

    CPC分类号： C12N1/22 ,  C08L1/02

    摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel.The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

    摘要翻译： 本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶， 为50％以上，以及其制造方法。 本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂， 凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。 提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。

    Solid cellulosic culture medium and process for producing the same
    19.
    发明申请
    Solid cellulosic culture medium and process for producing the same 有权
    固体纤维素培养基及其制备方法

    公开(公告)号：US20070172938A1

    公开(公告)日：2007-07-26

    申请号：US10591288

    申请日：2005-02-28

    申请人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

    发明人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

    IPC分类号： C12N1/20 ,  C08B5/00

    CPC分类号： C12N1/22 ,  C08L1/02

    摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel. The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

    摘要翻译： 本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶， 为50％以上，以及其制造方法。 本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂， 凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。 提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。