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公开(公告)号:US06565815B1
公开(公告)日:2003-05-20
申请号:US09194374
申请日:2000-07-25
申请人: Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人: Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
IPC分类号: F28D700
CPC分类号: G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要: The present invention provides a reaction vessel and apparatus for performing heat-exchanging reactions. The vessel has a chamber for holding a sample, the chamber being defined by a plurality of walls, at least two of the walls being light transmissive to provide optical windows to the chamber. The apparatus comprises at least one heating surface for contacting at least one of the plurality of walls, a heat source for heating the surface, and optics positioned to optically interrogate the chamber while the heating surface is in contact with at least one of the plurality of walls. The optics include at least one light source for transmitting light to the chamber through a first one of the light transmissive walls and at least one detector for detecting light exiting the chamber through a second one of the light transmissive walls.
摘要翻译: 本发明提供一种用于进行热交换反应的反应容器和装置。 容器具有用于保持样品的室,所述室由多个壁限定,至少两个壁是透光的,以向室提供光学窗口。 该装置包括用于接触多个壁中的至少一个的至少一个加热表面,用于加热该表面的热源,以及定位成光学询问该室的光学器件,同时该加热表面与多个壁板中的至少一个接触 墙壁 所述光学器件包括至少一个光源,用于通过第一透光壁将光传输到室,以及至少一个检测器,用于检测通过第二透光壁离开室的光。
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公开(公告)号:US09073053B2
公开(公告)日:2015-07-07
申请号:US11233629
申请日:2005-09-22
申请人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching
发明人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching
CPC分类号: B01L3/502 , B01L2200/10 , B01L2300/0681 , B01L2400/0487 , B01L2400/0644 , B01L2400/0655 , C12M47/06
摘要: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
摘要翻译: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器,以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子,用于破裂细胞或病毒。
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公开(公告)号:US20120295269A1
公开(公告)日:2012-11-22
申请号:US13552950
申请日:2012-07-19
申请人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
发明人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC分类号: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
摘要: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
摘要翻译: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室,其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径,所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室,从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径,将捕获的分析物从萃取室洗脱。
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公开(公告)号:US20120288924A1
公开(公告)日:2012-11-15
申请号:US13436475
申请日:2012-03-30
CPC分类号: B01L7/52 , B01L3/502 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0858 , B01L2300/087 , B01L2300/123 , B01L2300/14 , B01L2300/168 , B01L2300/18 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12Q1/6844 , G01N33/5302 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
摘要翻译: 用于进行化学反应的药筒包括具有形成在其中的至少一个流路的主体。 药筒还包括从身体延伸的反应容器,用于保持用于化学反应和光学检测的反应混合物。 容器包括限定反应室侧壁的刚性框架。 该框架包括连接到该腔室的流动路径的至少一个通道。 容器还包括附接到刚性框架的相对侧的柔性膜或片,以形成腔室的相对的主壁。 此外,至少两个侧壁是光学透射的并且与每个侧壁成角度地偏移以允许对反应室中的分析物的实时光学检测。
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公开(公告)号:US20110165562A1
公开(公告)日:2011-07-07
申请号:US13042203
申请日:2011-03-07
申请人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
发明人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC分类号: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
摘要: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
摘要翻译: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室,其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径,所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室,从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径,将捕获的分析物从萃取室洗脱。
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公开(公告)号:US20100068706A1
公开(公告)日:2010-03-18
申请号:US12370527
申请日:2009-02-12
申请人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
发明人: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC分类号: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
摘要: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
摘要翻译: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室,其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径,所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室,从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径,将捕获的分析物从萃取室洗脱。
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公开(公告)号:US20120171758A1
公开(公告)日:2012-07-05
申请号:US11977615
申请日:2007-10-24
申请人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US20120009667A1
公开(公告)日:2012-01-12
申请号:US13081060
申请日:2011-04-06
申请人: KURT E. PETERSON , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: KURT E. PETERSON , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US08029733B2
公开(公告)日:2011-10-04
申请号:US11981155
申请日:2007-10-30
申请人: Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人: Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
CPC分类号: G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要: A reaction vessel having a reaction chamber for holding a sample is fabricated by producing a housing having a rigid frame defining the minor walls of the chamber. The housing also defines a port for introducing fluid into the chamber. At least one sheet or film is attached to the rigid frame to form at least one major wall of the chamber. In preferred embodiments, two sheets or films are attached to opposite sides of the rigid frame to form two opposing major walls of the chamber, the major walls being connected to each other by the minor walls.
摘要翻译: 具有用于保持样品的反应室的反应容器通过制造具有限定室的小壁的刚性框架的壳体来制造。 壳体还限定了用于将流体引入腔室的端口。 至少一个片或薄膜连接到刚性框架以形成腔室的至少一个主壁。 在优选实施例中,两个片或膜连接到刚性框架的相对侧面以形成腔室的两个相对的主壁,主壁通过次壁彼此连接。
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公开(公告)号:US20080254532A1
公开(公告)日:2008-10-16
申请号:US11981155
申请日:2007-10-30
申请人: Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人: Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
IPC分类号: C12M1/02
CPC分类号: G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要: A reaction vessel having a reaction chamber for holding a sample is fabricated by producing a housing having a rigid frame defining the minor walls of the chamber. The housing also defines a port for introducing fluid into the chamber. At least one sheet or film is attached to the rigid frame to form at least one major wall of the chamber. In preferred embodiments, two sheets or films are attached to opposite sides of the rigid frame to form two opposing major walls of the chamber, the major walls being connected to each other by the minor walls.
摘要翻译: 具有用于保持样品的反应室的反应容器通过制造具有限定室的小壁的刚性框架的壳体来制造。 壳体还限定了用于将流体引入腔室的端口。 至少一个片或薄膜连接到刚性框架以形成腔室的至少一个主壁。 在优选实施例中,两个片或膜连接到刚性框架的相对侧面以形成腔室的两个相对的主壁,主壁通过次壁彼此连接。
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