公开(公告)号：US11867698B2

公开(公告)日：2024-01-09

申请号：US17027391

申请日：2020-09-21

Applicant: Life Technologies Corporation

Inventor： Jeffrey Dzubay ,  Kyle Gee ,  Vladimir Martin ,  Aleksey Rukavishnikov ,  Daniel Beacham

IPC: G01N33/58 ,  G01N33/50 ,  G01N33/84 ,  C09B11/24 ,  C09B11/28 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07D209/14 ,  C09K11/06 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B57/00 ,  C09B69/00 ,  G01N31/22 ,  G01N33/80

CPC classification number: G01N33/582 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/80 ,  G01N33/84 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:






wherein the variables are described throughout the application.

公开(公告)号：US10253185B2

公开(公告)日：2019-04-09

申请号：US15423890

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  C07D311/90 ,  A61K49/00 ,  G01N33/58 ,  G01N33/50 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US09939454B2

公开(公告)日：2018-04-10

申请号：US14937736

申请日：2015-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Dzubay ,  Kyle Gee ,  Vladimir Martin ,  Aleksey Rukavishnikov ,  Daniel Beacham

IPC: G01N33/84 ,  G01N33/58 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  G01N31/22 ,  C09B11/24 ,  G01N33/50 ,  G01N33/80 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07D209/14 ,  C09K11/06 ,  C07F5/02

CPC classification number: G01N33/84 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/80 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

公开(公告)号：US20160139135A1

公开(公告)日：2016-05-19

申请号：US14483392

申请日：2014-09-11

Applicant: Life Technologies Corporation

Inventor： Schuyler CORRY ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC: G01N33/58 ,  C07D501/60

CPC classification number: C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

Abstract translation: 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

公开(公告)号：US09164099B2

公开(公告)日：2015-10-20

申请号：US13890137

申请日：2013-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Aleksey Rukavishnikov ,  Courtenay Hart Kerndt ,  Richard Haugland ,  Wai-Yee Leung ,  Wayne Patton ,  Zhenjun Diwu

IPC: G01N33/533 ,  C07D239/00 ,  C07F5/02 ,  G01N33/58 ,  A61K49/00 ,  C07D311/12 ,  C07D311/18

CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

公开(公告)号：US20240042062A1

公开(公告)日：2024-02-08

申请号：US18380388

申请日：2023-10-16

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： Surbhi Desai ,  Marie Christine Nlend ,  Kyle Gee ,  Matthew Baker ,  Robert Aggeler ,  Scott Sweeney ,  Aleksey Rukavishnikov ,  Shih-Jung Huang

IPC: A61K49/00 ,  G01N33/58 ,  A61K47/10 ,  A61K47/18 ,  G01N33/533 ,  G01N33/68

CPC classification number: A61K49/0015 ,  G01N33/582 ,  A61K47/10 ,  A61K47/18 ,  A61K49/0058 ,  G01N33/533 ,  G01N33/68

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

公开(公告)号：US09910051B2

公开(公告)日：2018-03-06

申请号：US14455550

申请日：2014-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel Beacham ,  Jeffrey Dzubay ,  Kyle Gee ,  Vladimir Martin ,  Aleksey Rukavishnikov

IPC: G01N33/84 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  G01N31/22 ,  C09B11/24 ,  G01N33/50 ,  G01N33/58 ,  G01N33/80 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07D209/14 ,  C09K11/06 ,  C07F5/02

CPC classification number: G01N33/84 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/80 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

公开(公告)号：US20160025713A1

公开(公告)日：2016-01-28

申请号：US14876782

申请日：2015-10-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Courtenay Hart-Kerndt ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

IPC: G01N33/52 ,  C07F5/02 ,  C07D311/16 ,  G01N33/58

CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

公开(公告)号：US09140706B2

公开(公告)日：2015-09-22

申请号：US13972081

申请日：2013-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan Antoulinakis ,  Kyle Gee ,  Aleksey Rukavishnikov

IPC: C07D311/80 ,  C07D311/82 ,  C07D311/88 ,  G01N33/58 ,  C07D209/14 ,  C07D401/10 ,  G01N33/532 ,  C09B11/26 ,  C09B45/00

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.

Abstract translation: 本公开涉及能够将报告分子与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有以下通式：其中在整个申请中描述了变量。

公开(公告)号：US20130337536A1

公开(公告)日：2013-12-19

申请号：US13972081

申请日：2013-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan Antoulinakis ,  Kyle Gee ,  Aleksey Rukavishnikov

IPC: G01N33/58 ,  C09B11/26 ,  C09B45/00

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.