中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

15 records (took 0.019 seconds)

    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT
    12.
    发明申请
    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT 审中-公开

    公开(公告)号：US20170101668A1

    公开(公告)日：2017-04-13

    申请号：US15387525

    申请日：2016-12-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian REED

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  C07H1/06 ,  C12N15/1013 ,  C12Q1/6844 ,  C12Q2537/163 ,  C12Q2563/159

    Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.

    Systems and Methods for Substrate Enrichment
    13.
    发明申请
    Systems and Methods for Substrate Enrichment 有权
    基质富集系统与方法

    公开(公告)号：US20150361418A1

    公开(公告)日：2015-12-17

    申请号：US14736734

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian REED

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1013 ,  C07H1/06 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2563/143 ,  C12Q2563/157 ,  C12Q2565/519

    Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.

    Abstract translation: 分离珠粒基材的方法包括将乳液施加到破乳溶液中。 乳液的分散相包括未结合的多核苷酸，第一组珠粒基质和第二组珠粒基底。 未结合的多核苷酸包括与偶联寡核苷酸互补的区段。 第一组珠底物包括扩增为包含与未结合多核苷酸的一部分互补的区段的偶联寡核苷酸。 第二组珠底物包括偶联寡核苷酸。 破乳溶液包括具有与偶联寡核苷酸相似的序列或与偶联寡核苷酸互补的序列的干扰探针。 该方法还包括将第一组胎圈衬底的珠粒与分离衬底结合，并将第二组胎圈衬底的未结合的珠粒与与分离衬底结合的第一组胎圈衬底的珠粒分离。

    System and Method for Emulsion Breaking
    14.
    发明申请
    System and Method for Emulsion Breaking 有权
    乳液破裂的系统和方法

    公开(公告)号：US20150133649A1

    公开(公告)日：2015-05-14

    申请号：US14536429

    申请日：2014-11-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian REED ,  James A. Ball

    IPC: B01D17/04 ,  C07H21/00 ,  C07H1/06

    CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

    Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，乳液破碎和分散相的材料优先分配至表面活性剂溶液。

Patent Agency Ranking