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    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    11.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20180305673A1

    公开(公告)日：2018-10-25

    申请号：US15959839

    申请日：2018-04-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lloyd Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6844

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Polymerase Compositions, Methods of Making and Using Same
    13.
    发明申请
    Polymerase Compositions, Methods of Making and Using Same 有权
    聚合酶组合物，制备和使用方法

    公开(公告)号：US20150368626A1

    公开(公告)日：2015-12-24

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。 在一些方面，本公开涉及可用于产生用于各种下游过程的核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及可以跨聚合酶类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的催化性质的新型聚合酶。 在一些方面，本公开提供与参考聚合酶相比具有增强的催化性质的修饰聚合酶。

    Methods and systems for modeling phasing effects in sequencing using termination chemistry
    15.
    发明授权
    Methods and systems for modeling phasing effects in sequencing using termination chemistry 有权

    公开(公告)号：US10410739B2

    公开(公告)日：2019-09-10

    申请号：US14506520

    申请日：2014-10-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Christian Koller ,  Marcin Sikora ,  Peter Vander Horn

    IPC: G16B40/10 ,  C12Q1/6869 ,  G16B30/00 ,  G16B5/00 ,  G16B20/00 ,  G16B25/00

    Abstract: A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, one candidate sequence leading to optimization of a solver function as corresponding to the sequence for the sample nucleic acid.

    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME
    17.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME 有权
    聚合物组合物及其制备方法和使用方法

    公开(公告)号：US20160177373A1

    公开(公告)日：2016-06-23

    申请号：US14970818

    申请日：2015-12-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12Q1/68 ,  C12N9/12

    CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q1/6869 ,  C12Q2521/101 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a reference Taq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了修饰的聚合酶和其生物活性片段，例如修饰的Taq聚合酶，其允许改善的核酸扩增。 在一些方面，本公开提供与参考Taq聚合酶相比具有改进的热稳定性，准确性，持续性和/或读取长度的修饰聚合酶。 在一些方面，本公开涉及可用于扩增方法的修饰聚合酶或其生物活性片段，并且在实际示例性实施方案中涉及乳液PCR。

    Methods and systems for modeling phasing effects in sequencing using termination chemistry
    18.
    发明授权
    Methods and systems for modeling phasing effects in sequencing using termination chemistry 有权

    公开(公告)号：US11636922B2

    公开(公告)日：2023-04-25

    申请号：US16550374

    申请日：2019-08-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Christian Koller ,  Marcin Sikora ,  Peter Vander Horn

    IPC: G16B40/10 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6869 ,  G16B5/00

    Abstract: A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, one candidate sequence leading to optimization of a solver function as corresponding to the sequence for the sample nucleic acid.

    Polymerase compositions, methods of making and using same
    20.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US11208636B2

    公开(公告)日：2021-12-28

    申请号：US16850629

    申请日：2020-04-16

    Applicant: Life Technologies Corporation

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

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