公开(公告)号：US20190119738A1

公开(公告)日：2019-04-25

申请号：US16168810

申请日：2018-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan M. ROTHBERG ,  Bin LI ,  Kai Qin LAO

IPC: C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853

Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20170175171A1

公开(公告)日：2017-06-22

申请号：US15449192

申请日：2017-03-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey SABINA ,  Ilya ZLATKOVSKY ,  Rachel KASINSKAS

IPC: C12Q1/68 ,  G01N1/40

CPC classification number: C07H21/00 ,  C07K1/145 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N1/4055 ,  C12Q2523/32 ,  C12Q2563/161 ,  C12Q2535/122

Abstract: The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.

公开(公告)号：US20140148345A1

公开(公告)日：2014-05-29

申请号：US14085718

申请日：2013-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6846 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/519 ,  C12Q2565/607

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20210261929A1

公开(公告)日：2021-08-26

申请号：US17302192

申请日：2021-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

IPC: C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686

Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20160272954A1

公开(公告)日：2016-09-22

申请号：US15091717

申请日：2016-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO ,  Wolfgang HINZ

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20160032375A1

公开(公告)日：2016-02-04

申请号：US14789922

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6846 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/519 ,  C12Q2565/607

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20150275284A1

公开(公告)日：2015-10-01

申请号：US14692706

申请日：2015-04-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Bin LI ,  Kai Qin LAO ,  Jennifer O'NEIL ,  Jennifer KUNKEL ,  Kellie HALEY ,  Rachel KASINSKAS ,  Zhaochun MA ,  Pius BRZOSKA

IPC: C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。

公开(公告)号：US20130281307A1

公开(公告)日：2013-10-24

申请号：US13923232

申请日：2013-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.