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    Ligational encoding of small molecules
    13.
    发明申请
    Ligational encoding of small molecules 有权
    小分子的编码编码

    公开(公告)号：US20060246450A1

    公开(公告)日：2006-11-02

    申请号：US10549619

    申请日：2004-03-22

    申请人： Thomas Franch ,  Soeren Jacobsen ,  Torben Rasmussen ,  Soeren Neve ,  Henrik Pedersen ,  Alex Gouliaev

    发明人： Thomas Franch ,  Soeren Jacobsen ,  Torben Rasmussen ,  Soeren Neve ,  Henrik Pedersen ,  Alex Gouliaev

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C07H21/04 ,  C12N15/1068 ,  C12P21/02 ,  C40B40/06 ,  C40B50/04

    摘要： The invention relates to a method for synthesising a bifunctional complex comprising an encoded molecule and an identifier polynucleotide identifying the chemical entities having participated in the synthesis of the encoded molecule, said method comprising the steps of i) providing a) at least one template comprising one or more codons capable of hybridising to an anti-codon, wherein said template is optionally associated with one or more chemical entities, and b) a plurality of building blocks each comprising an anti-codon associated with one or more chemical entities, and ii) hybridising the anti-codon of one or more of the provided building blocks to the template, iii) covalently linking said anti-codons and/or linking the at least one template with the anti-codon of at least one building block, thereby generating an identifier polynucleotide capable of identifying chemical entities having participated in the synthesis of the encoded molecule, iv) separating the template from one or more of the anti-codons hybridised thereto, thereby generating an at least partly single stranded identifier polynucleotide associated with a plurality of chemical entities, v) generating a bifunctional complex comprising an encoded molecule and an identifier polynucleotide identifying the chemical entities having participated in the synthesis of the encoded molecule, wherein said encoded molecule is generated by reacting at least two of said plurality of chemical entities associated with the identifier polynucleotide, and wherein said at least two chemical entities are provided by separate building blocks.

    摘要翻译： 本发明涉及一种合成双功能复合物的方法，所述双功能复合物包含编码分子和识别已经参与编码分子合成的化学实体的标识多核苷酸，所述方法包括以下步骤：i）提供a）至少一个包含一个 或更多能够与抗密码子杂交的密码子，其中所述模板任选地与一个或多个化学实体相关联，以及b）多个结构单元，每个结构单元包含与一个或多个化学实体相关的抗密码子，以及ii） 将一个或多个所提供的构建块的抗密码子与模板杂交，iii）共价连接所述抗密码子和/或将至少一个模板与至少一个构建块的抗密码子连接，由此产生 能够识别已经参与编码分子的合成的化学实体的标识符多核苷酸，iv）将模板从一个或多个分离出来 更多的与其杂交的抗密码子，从而产生与多个化学实体相关联的至少部分单链标识多核苷酸，v）产生包含编码分子的双功能复合物和识别参与合成的化学实体的标识符多核苷酸 的编码分子，其中所述编码的分子通过使与所述标识多核苷酸相关联的所述多个化学实体中的至少两个反应而产生，并且其中所述至少两个化学实体由单独的构建块提供。

    Method of producing a polypeptide using oxaloacetate hydrolase deficient fungal host cells
    16.
    发明授权
    Method of producing a polypeptide using oxaloacetate hydrolase deficient fungal host cells 有权
    使用草酰乙酸水解酶缺陷型真菌宿主细胞产生多肽的方法

    公开(公告)号：US06936438B2

    公开(公告)日：2005-08-30

    申请号：US10336491

    申请日：2003-01-03

    申请人： Carsten Mailand Hjort ,  Henrik Pedersen

    发明人： Carsten Mailand Hjort ,  Henrik Pedersen

    IPC分类号： C12N1/21 ,  C12N9/14 ,  C12N15/55 ,  C12N21/06 ,  C12N9/10 ,  C12N15/63

    CPC分类号： C12Q1/6895 ,  C12N9/14

    摘要： The present invention relates to methods of producing a polypeptide comprising culturing a mutant of a parent cell, wherein the mutant produces less oxaloacetate hydrolase than the parent cell, wherein the oxaloacetate hydrolase (i) has an amino acid sequence that has at least 90% identity with amino acids 1-341 of SEQ ID NO: 2: (ii) is encoded by a nucleic acid sequence having at least 90% homology with a nucleotide sequence comprising nuclotides 1157-1411, 1504-1651 and 1764-2383 of SEQ ID NO: 1: (iii) is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the nucleic acid sequence of SEQ ID NO: 1, the cDNA sequence of SEQ ID NO: 1, the complementary strand of SEQ ID NO: 1, and/or the complementary strand of the cDNA sequence of SEQ ID NO: 1: and/or (iv) a subsequence of one or both of (i) and (ii), wherein the subsequence encodes a fragment that has oxaloacetate hydrolase activity.

    摘要翻译： 本发明涉及生产多肽的方法，其包括培养亲本细胞的突变体，其中所述突变体比母体细胞产生较少的草酰乙酸水解酶，其中草酰乙酸水解酶（i）具有至少90％同一性的氨基酸序列 与SEQ ID NO：2的氨基酸1-341：（ii）由与SEQ ID NO的核苷酸1157-1411,1504-1651和1764-2383的核苷酸序列具有至少90％同源性的核酸序列编码 1：（iii）由高度严格条件下与SEQ ID NO：1的核酸序列，SEQ ID NO：1的cDNA序列，SEQ ID NO：1的互补链杂交的核酸序列编码 ，和/或SEQ ID NO：1的cDNA序列的互补链和/或（iv）（i）和（ii）中的一个或两个的亚序列，其中所述亚序列编码具有草酰乙酸水解酶活性的片段 。

    METHOD FOR IDENTIFICATION OF MOLECULES WITH DESIRED CHARACTERISTICS
    20.
    发明申请
    METHOD FOR IDENTIFICATION OF MOLECULES WITH DESIRED CHARACTERISTICS 审中-公开

    公开(公告)号：US20180340174A1

    公开(公告)日：2018-11-29

    申请号：US15525681

    申请日：2015-11-11

    申请人： Mikkel Dybro Lundorf ,  Henrik Pedersen ,  NANOCORE APS

    发明人： Mikkel Dybro Lundorf ,  Henrik Pedersen ,  Tore Dehli

    IPC分类号： C12N15/10

    摘要： A method for identifying molecules with desired characteristics such as high affinity for a surface or material is described. A particularly useful method covered by the present invention allows identification of molecules which bind a material with high affinity in the presence of fluid or soluble polymers, such that said molecules can be used to produce a composite in which they efficiently anchor a material in a matrix comprising solid forms of a polymer. Compositions/kits useful for identification of molecules with desired characteristics are also described.