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41 条记录 (耗时 0.023 秒)

    Method for immobolizing molecules on surfaces
    11.
    发明申请
    Method for immobolizing molecules on surfaces 审中-公开
    表面上固定分子的方法

    公开(公告)号：US20050158848A1

    公开(公告)日：2005-07-21

    申请号：US10511192

    申请日：2003-04-10

    申请人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

    发明人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

    IPC分类号： B01J20/32 ,  C09D125/06 ,  C09D179/08 ,  C09D201/00 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  B05D3/00

    CPC分类号： B01J20/3204 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/3257 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3289 ,  G01N33/54393

    摘要： In a method for immobilizing molecules on a surface, a layer of a hydrophobic, e.g., a non-swelling polymer, may be applied to the surface and molecules are immobilized on a surface of the polymer layer. The hydrophobic polymers may be polyimide or polystyrene, and the surface to which the polymer layer is applied may be made of an inorganic material, e.g., a semiconductor material (silicon), a semiconducting oxide (silicon dioxide), glass, nitride, or ceramic. Hydrophobic polymers such as polyimide or polystyrene can be applied to the surface of an inorganic support with conventional methods. Further, they electrically insulate the support with respect to the molecules applied to the surface of the polymer layer or substances associated with these molecules. Thus, electrical sensors and processor circuits can be integrated into the support without any detrimental effect on their function due to molecules and substances applied to the surface of the polymer layer.

    摘要翻译： 在将分子固定在表面上的方法中，可以将一层疏水的，例如不溶胀的聚合物施加到表面上，并将分子固定在聚合物层的表面上。 疏水聚合物可以是聚酰亚胺或聚苯乙烯，并且施加聚合物层的表面可以由无机材料制成，例如半导体材料（硅），半导体氧化物（二氧化硅），玻璃，氮化物或陶瓷 。 疏水性聚合物如聚酰亚胺或聚苯乙烯可以用常规方法施加到无机载体的表面上。 此外，它们相对于施加到聚合物层的表面上的分子或与这些分子相关联的物质将载体电绝缘。 因此，电传感器和处理器电路可以集成到支撑件中，而不会由于施加到聚合物层表面的分子和物质而对它们的功能产生不利影响。

    Device and method for detecting cellular processes by means of luminescence measurements
    14.
    发明授权
    Device and method for detecting cellular processes by means of luminescence measurements 有权
    通过发光测量检测细胞过程的装置和方法

    公开(公告)号：US07767443B2

    公开(公告)日：2010-08-03

    申请号：US10509040

    申请日：2003-03-05

    申请人： Mirko Lehmann ,  Holger Klapproth

    发明人： Mirko Lehmann ,  Holger Klapproth

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： C12M41/46

    摘要： A device includes a carrier element with a surface prepared for direct or indirect coupling or receiving of cells, at least one optical detector to receive a luminescence signal, the detector being integrated into the carrier element below the prepared surface, a cover covering the prepared surface to form a cavity, the cover having an inlet opening and an outlet opening, and an excitation source connected to the inlet opening. The excitation source constitutes a reservoir for a chemical or biological excitation substance or medium that influences the metabolism of the cell, with metabolic processes being made visible by luminescence and detected by at the least one optical detector.

    摘要翻译： 一种装置包括具有准备用于直接或间接耦合或接收细胞的表面的载体元件，至少一个用于接收发光信号的光学检测器，所述检测器被集成到所述制备表面下方的载体元件中，覆盖所制备的表面 为了形成空腔，盖具有入口开口和出口，以及连接到入口的激励源。 激发源构成用于影响细胞代谢的化学或生物激发物质或介质的储存器，代谢过程通过发光使其可见并且由至少一个光学检测器检测。

    Integrated sensor arrangement
    16.
    发明申请
    Integrated sensor arrangement 审中-公开
    集成传感器布置

    公开(公告)号：US20070107514A1

    公开(公告)日：2007-05-17

    申请号：US11545778

    申请日：2006-10-10

    申请人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

    发明人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

    IPC分类号： G01F15/14

    CPC分类号： G01N21/6454

    摘要： An integrated sensor array has a substrates, at least one sensor element, and a signal processing device integrated in the substrate and connected or capable of being connected with the sensor elements. A layer having a measuring surface is capable of being detachably bonded to the substrate so that a signal emitted from the measuring surface is capable of being processed by the signal processing device when the layer is bonded to the substrate. The substrate has at least one feedthrough hole abutting with the layer, which feedthrough hole is connected or capable of being connected to a vacuum generating device in order to bond the layer to the substrate by suction.

    摘要翻译： 集成传感器阵列具有基板，至少一个传感器元件和集成在基板中并连接或能够与传感器元件连接的信号处理装置。 具有测量表面的层能够可拆卸地结合到基板，使得当该层与基板结合时，从测量表面发射的信号能够被信号处理装置处理。 衬底具有与该层邻接的至少一个馈通孔，所述馈通孔连接或能够连接到真空产生装置，以便通过抽吸将层粘合到衬底。

    Method and device for the detection of at least one luminescent substance
    17.
    发明申请
    Method and device for the detection of at least one luminescent substance 有权
    用于检测至少一种发光物质的方法和装置

    公开(公告)号：US20060055042A1

    公开(公告)日：2006-03-16

    申请号：US10529048

    申请日：2003-09-12

    申请人： Holger Klapproth ,  Mirko Lehmann

    发明人： Holger Klapproth ,  Mirko Lehmann

    IPC分类号： H01L23/48

    CPC分类号： G01N21/6454 ,  G01N21/6428 ,  G01N21/648 ,  H01L2924/0002 ,  H01L2924/00

    摘要： Disclosed is a device for detecting at least one luminescent substance, comprising a radiation source for emitting excitation radiation to the at least one luminescent substance. The excitation radiation is provided with at least one excitation wavelength at which the luminescent substance is excited so as to emit luminescent radiation. At least one radiation receiver is provided, which detects the luminescent radiation and is configured regarding the spectral sensitivity thereof in such a way that said radiation receiver is insensitive to the radiation emitted by the radiation source. The luminescent substance is located inside a measuring chamber that is essentially impermeable to the luminescent radiation and comprises at least one wall area which is transparent to the excitation radiation emitted by the radiation source. The radiation source is placed outside the measuring chamber such that the excitation radiation emitted by the radiation source is coupled into the inside of the measuring chamber by penetrating said wall area.

    摘要翻译： 公开了一种用于检测至少一种发光物质的装置，包括用于向至少一种发光物质发射激发辐射的辐射源。 激发辐射具有至少一个激发波长，在该激发波长处激发发光物质以发射发光辐射。 提供至少一个辐射接收器，其检测发光辐射，并且被配置为关于其光谱灵敏度，使得所述辐射接收器对由辐射源发射的辐射不敏感。 发光物质位于测量室内，其基本上不透气于发光辐射，并且包括对由辐射源发射的激发辐射透明的至少一个壁区域。 辐射源被放置在测量室的外部，使得由辐射源发射的激发辐射通过穿透所述壁区域而耦合到测量室的内部。

    Device and method for detecting cellular processes by means of luminescence measurements
    18.
    发明申请
    Device and method for detecting cellular processes by means of luminescence measurements 有权
    通过发光测量检测细胞过程的装置和方法

    公开(公告)号：US20050153387A1

    公开(公告)日：2005-07-14

    申请号：US10509040

    申请日：2003-03-05

    申请人： Mirko Lehmann ,  Holger Klapproth

    发明人： Mirko Lehmann ,  Holger Klapproth

    IPC分类号： C12M1/34 ,  C12Q1/02 ,  G01N33/487 ,  G01N33/50 ,  G01N33/543 ,  G01N1/30 ,  G01N33/48

    CPC分类号： C12M41/46

    摘要： The invention relates to a device for detecting a luminescence event in or in the immediate vicinity of a cell, a cell cluster, or a tissue, having the following elements: (a) a carrier element (1) having a surface (100) prepared for direct or indirect coupling of cells, and (b) at least one optical detector (2) for receiving a luminescence signal and integrated in the carrier element (1) below the surface (100), characterized by the following elements: (c) a cover (7) having an inlet (8) and an outlet (9) and covering the surface (100) such as to form a cavity (70), and (d) an excitation source (21) connected to the inlet (8).

    摘要翻译： 本发明涉及一种用于检测细胞，细胞簇或组织紧邻附近的发光事件的装置，具有以下元件：（a）具有制备表面（100）的载体元件（1） 用于单元的直接或间接耦合，以及（b）用于接收发光信号并集成在所述表面（100）下方的载体元件（1）中的至少一个光学检测器（2），其特征在于以下元件：（c） 具有入口（8）和出口（9）并覆盖所述表面（100）以形成空腔（70）的盖（7），和（d）连接到所述入口（8）的激发源 ）。

    Method For Regenerating A Biosensor
    19.
    发明申请
    Method For Regenerating A Biosensor 失效
    生物传感器再生方法

    公开(公告)号：US20100129894A1

    公开(公告)日：2010-05-27

    申请号：US12619871

    申请日：2009-11-17

    申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

    发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

    IPC分类号： C07K1/00

    CPC分类号： G01N33/54393

    摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

    摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。

    Method for regenerating a biosensor
    20.
    发明授权
    Method for regenerating a biosensor 失效
    生物传感器再生方法

    公开(公告)号：US08518709B2

    公开(公告)日：2013-08-27

    申请号：US12619871

    申请日：2009-11-17

    申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

    发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

    IPC分类号： G01N21/00

    CPC分类号： G01N33/54393

    摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

    摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。