公开(公告)号：US20160289655A1

公开(公告)日：2016-10-06

申请号：US15185250

申请日：2016-06-17

Applicant: NOVOZYMES A/S

Inventor： Leonardo De Maria ,  Lars Kobberoee Skov ,  Michael Skjoet

IPC: C12N9/16 ,  A23K20/189 ,  A23K50/80 ,  A23K50/10 ,  A23K50/30 ,  A23K50/75 ,  C12P7/06 ,  A23K20/147

CPC classification number: C12N9/16 ,  A23K20/147 ,  A23K20/189 ,  A23K50/10 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/10 ,  A23L33/15 ,  A23L33/16 ,  A23V2002/00 ,  C12N15/8243 ,  C12P7/06 ,  Y02A40/818 ,  Y02E50/17

Abstract: The present invention relates to a phytase which has at least 70% identity to a phytase derived from E. coli and comprises at least one modification as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

Abstract translation: 本发明涉及与来自大肠杆菌的植酸酶具有至少70％同一性的植酸酶，并且与该植酸酶相比包含至少一种修饰。 这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

公开(公告)号：US09451783B2

公开(公告)日：2016-09-27

申请号：US14504517

申请日：2014-10-02

Applicant: Novozymes A/S

Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

IPC: C12N9/16 ,  A23K1/165

CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

公开(公告)号：US08877471B2

公开(公告)日：2014-11-04

申请号：US13874954

申请日：2013-05-01

Applicant: Novozymes A/S

Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

IPC: C12N9/00 ,  C12N9/16 ,  A23K1/165

CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。

公开(公告)号：US10986850B2

公开(公告)日：2021-04-27

申请号：US16463205

申请日：2017-12-21

Applicant: Novozymes A/S

Inventor： Mikkel Klausen ,  Kirk Matthew Schnorr ,  Lars Kobberoee Skov ,  Soeren Nymand-Grarup ,  Marianne Thorup Cohn ,  Peter Bjarke Olsen ,  Ming Li ,  Ye Liu

IPC: A23K20/189 ,  A23K20/174 ,  A23K20/22 ,  A23K20/24 ,  A23K20/20 ,  A23K50/30 ,  A23K50/75 ,  A23K10/30 ,  C12N9/36

Abstract: Animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity are disclosed. Polypeptides having lysozyme activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides are also provided.

公开(公告)号：US10066220B2

公开(公告)日：2018-09-04

申请号：US15342856

申请日：2016-11-03

Applicant: NOVOZYMES A/S

Inventor： Bjarne Gram Hansen ,  Steffen Danielsen ,  Lars Kobberoee Skov ,  Leonardo De Maria ,  Julie Bille Rannes

IPC: A62D3/02 ,  A62D101/02 ,  A62D101/04 ,  C12N9/16 ,  A62D3/30 ,  A62D101/26

CPC classification number: C12N9/16 ,  A62D3/02 ,  A62D3/30 ,  A62D2101/02 ,  A62D2101/04 ,  A62D2101/26 ,  C12Y301/08002

Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20160362666A1

公开(公告)日：2016-12-15

申请号：US15249856

申请日：2016-08-29

Applicant: Novozymes A/S

Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

IPC: C12N9/16 ,  A23K20/189

CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensibility, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

公开(公告)号：US09447392B2

公开(公告)日：2016-09-20

申请号：US14209463

申请日：2014-03-13

Applicant: NOVOZYMES A/S

Inventor： Steffen Danielsen ,  Lars Kobberoee Skov ,  Ricardo Leite ,  Vincent Laize ,  M. Leonor Cancela Da Fonseca

IPC: C12N9/16 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C12P21/06 ,  A62D3/00 ,  C07H21/04 ,  C07K1/00 ,  A62D3/02 ,  A62D101/04 ,  A62D101/26 ,  A62D101/02

CPC classification number: C12N9/16 ,  A62D3/02 ,  A62D2101/02 ,  A62D2101/04 ,  A62D2101/26 ,  C12Y301/08001 ,  C12Y301/08002

Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有有机磷水解酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US20210153523A1

公开(公告)日：2021-05-27

申请号：US17153096

申请日：2021-01-20

Applicant: Novozymes A/S

Inventor： Ye Liu ,  Kirk Matthew Schnorr ,  Lars Kiemer ,  Lars Kobberoee Skov ,  Dorthe Hoej Sandvang ,  Marianne Thorup Cohn ,  Ming Li

IPC: A23K20/189 ,  A23K20/147 ,  A23K20/174 ,  A23K20/28 ,  C12N9/36 ,  A23K50/60 ,  A61K38/47 ,  A23K50/80 ,  A23K50/75 ,  A23K50/30

Abstract: The present invention relates animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also relates to polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US10945449B2

公开(公告)日：2021-03-16

申请号：US15579769

申请日：2016-07-04

Applicant: Novozymes A/S

Inventor： Ye Liu ,  Kirk Matthew Schnorr ,  Lars Kiemer ,  Lars Kobberoee Skov ,  Dorthe Hoej Sandvang ,  Marianne Thorup Cohn ,  Ming Li

IPC: C12N9/24 ,  A23K20/189 ,  A23K20/147 ,  A23K20/174 ,  A23K20/28 ,  C12N9/36 ,  A23K50/60 ,  A61K38/47 ,  A23K50/80 ,  A23K50/75 ,  A23K50/30

Abstract: The present invention provides animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also provides polypeptides having lysozyme activity, polynucleotides encoding the polypeptides as well as methods of producing and using the polypeptides.

公开(公告)号：US09611469B2

公开(公告)日：2017-04-04

申请号：US14627584

申请日：2015-02-20

Applicant: NOVOZYMES A/S

Inventor： Lars Kobberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

IPC: C12N9/96 ,  C12N9/16 ,  C12P7/00 ,  A61K38/46 ,  C12N15/82 ,  C12P7/14 ,  C12P21/00 ,  A23K10/16 ,  A23L33/195

CPC classification number: C12N9/96 ,  A23K10/16 ,  A23L33/195 ,  A61K38/46 ,  C12N9/16 ,  C12N15/8243 ,  C12P7/00 ,  C12P7/14 ,  C12P21/00 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072

Abstract: The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.