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38 条记录 (耗时 0.021 秒)

    Reduction of non-specific binding in immunoassays
    12.
    发明申请
    Reduction of non-specific binding in immunoassays 审中-公开
    免疫测定中非特异性结合的减少

    公开(公告)号：US20080108147A1

    公开(公告)日：2008-05-08

    申请号：US11592558

    申请日：2006-11-03

    申请人： Tie Wei ,  Pratap Singh ,  James Duffy ,  Amy Posey

    发明人： Tie Wei ,  Pratap Singh ,  James Duffy ,  Amy Posey

    IPC分类号： G01N33/566 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/535 ,  G01N33/563

    CPC分类号： G01N33/54313 ,  C07K16/26 ,  G01N33/54393

    摘要： Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample wherein one of the reagents for conducting the binding assay is an antibody reagent. The methods comprise treating the antibody reagent at a pH of about 2.0 to about 3.5. The method may further comprise treating the antibody reagent with a reducing agent in an amount sufficient to reduce non-specific binding of the antibody reagent. In some embodiments the reducing agent may be a thiol-containing reducing agent or a combination of two or more thiol-containing reducing agents.

    摘要翻译： 公开了用于在用于测定样品中分析物的结合测定中的非特异性结合的方法和组合物，其中用于进行结合测定的试剂之一是抗体试剂。 所述方法包括在约2.0至约3.5的pH下处理抗体试剂。 该方法可以进一步包括用足以减少抗体试剂的非特异性结合的量的还原剂处理抗体试剂。 在一些实施方案中，还原剂可以是含硫醇的还原剂或两种或更多种含硫醇的还原剂的组合。

    Immunoassays for buprenorphine and norbuprenorphine
    13.
    发明授权
    Immunoassays for buprenorphine and norbuprenorphine 有权
    丁丙诺啡和诺布啡诺的免疫测定

    公开(公告)号：US07220842B2

    公开(公告)日：2007-05-22

    申请号：US10818115

    申请日：2004-04-05

    申请人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

    发明人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

    IPC分类号： C07K17/02 ,  C07D491/02 ,  G01N33/535

    CPC分类号： C07K16/44 ,  G01N33/9486

    摘要： Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

    摘要翻译： 公开了针对丁丙诺啡（BUP）和丁丙诺啡（norBUP）的半抗原，免疫原和免疫测定法的方法，组合物和试剂盒。 该方法包括在介质中提供（i）怀疑含有丁丙诺啡（BUP）或降丁非诺（norBUP））的样品和（ii）针对丁丙诺啡（BUP）或去诺贝芬（norBUP）的免疫原产生的抗体。 检查介质是否存在包含丁丙诺啡（BUP）或降丁非诺（norBUP）的标记半抗原的复合物，其中诸如复合物的存在表明样品中存在化合物。

    Demountable heat sink
    14.
    发明授权
    Demountable heat sink 失效
    可拆卸散热器

    公开(公告)号：US5654876A

    公开(公告)日：1997-08-05

    申请号：US583322

    申请日：1996-01-05

    申请人： Sanjeev Balwant Sathe ,  Pratap Singh

    发明人： Sanjeev Balwant Sathe ,  Pratap Singh

    IPC分类号： H01L23/40 ,  H05M7/20

    CPC分类号： H01L23/4006 ,  H01L2224/73253

    摘要： A demountable, and thus re-usable, conductive heat sink for dissipation of excess heat of an electronic component. The heat sink uses a T-bolt and T-slot for attachment to the electronic component and the printed circuit board, respectively. This attachment eliminates the typical concerns associated with the placement of traces on the PCB to accommodate the holes placed therein for the attachment of conventional heat sinks. The improved designed also facilitates demounting of the heat sink from the printed circuit board and the electronic component without damage thereto.

    摘要翻译： 可拆卸且因此可重复使用的导电散热器，用于消散电子部件的多余热量。 散热器分别使用T型螺栓和T型槽连接到电子部件和印刷电路板。 该附件消除了与PCB上的迹线放置相关的典型问题，以容纳放置在其中以用于附接常规散热器的孔。 改进的设计还有助于散热器从印刷电路板和电子部件的拆卸而不损坏其。

    Signal Ratio in Assay Calibrators
    15.
    发明申请
    Signal Ratio in Assay Calibrators 审中-公开
    测定校准物中的信号比

    公开(公告)号：US20140212987A1

    公开(公告)日：2014-07-31

    申请号：US13753909

    申请日：2013-01-30

    申请人： Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

    发明人： Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

    IPC分类号： G01N33/82

    CPC分类号： G01N33/82 ,  G01N33/5306 ,  G01N33/6854

    摘要： Methods of enhancing signal ratio between calibrators in an assay for an analyte include conducting an assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level. The reagents employed in the assay comprise an antibody reagent comprising an antibody for the analyte wherein a hinge region of the antibody is conjugated to a moiety. The assay for the analyte is also conducted with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second analyte concentration is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent. A ratio of the first signal level to the second signal level is determined and evaluated.

    摘要翻译： 在分析物测定中增强校准器之间的信号比率的方法包括在第一校准器中对零分析物进行分析物测定以确定第一信号水平。 在测定中使用的试剂包含抗体试剂，其包含用于分析物的抗体，其中抗体的铰链区域与部分缀合。 分析物的测定还用第二校准器中的第二浓度的分析物进行，以确定第二分析物浓度大于零的第二信号水平，并且其中测定中使用的试剂包含抗体试剂。 确定并评估第一信号电平与第二信号电平的比率。

    Immunoassays for buprenorphine and norbuprenorphine
    16.
    发明申请
    Immunoassays for buprenorphine and norbuprenorphine 有权
    丁丙诺啡和诺布啡诺的免疫测定

    公开(公告)号：US20050221405A1

    公开(公告)日：2005-10-06

    申请号：US10818115

    申请日：2004-04-05

    申请人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

    发明人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

    IPC分类号： G01N33/53 ,  G01N33/94

    CPC分类号： C07K16/44 ,  G01N33/9486

    摘要： Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

    摘要翻译： 公开了针对丁丙诺啡（BUP）和丁丙诺啡（norBUP）的半抗原，免疫原和免疫测定法的方法，组合物和试剂盒。 该方法包括在介质中提供（i）怀疑含有丁丙诺啡（BUP）或降丁非诺（norBUP））的样品和（ii）针对丁丙诺啡（BUP）或去诺贝芬（norBUP）的免疫原产生的抗体。 检查介质是否存在包含丁丙诺啡（BUP）或降丁非诺（norBUP）的标记半抗原的复合物，其中诸如复合物的存在表明样品中存在化合物。

    Reconstitution of purified membrane proteins into preformed liposomes
    18.
    发明授权
    Reconstitution of purified membrane proteins into preformed liposomes 有权
    将纯化的膜蛋白重建成预先形成的脂质体

    公开(公告)号：US06706861B2

    公开(公告)日：2004-03-16

    申请号：US09815195

    申请日：2001-03-22

    申请人： Pratap Singh ,  Jianfang Wang ,  Liliana Maria Tejidor

    发明人： Pratap Singh ,  Jianfang Wang ,  Liliana Maria Tejidor

    IPC分类号： A61K3514

    CPC分类号： G01N33/86 ,  A61K9/1271 ,  A61K9/1278 ,  Y02A50/473 ,  Y10S436/829

    摘要： The present application relates to a method of making liposomes having membrane proteins incorporated therein, the method comprising: providing the membrane protein in solution; providing a solution of preformed liposomes; and incubating the mixture. Prior to the step of providing a solution of preformed liposomes, the liposomes are formed by combining a mixture of phospholipids with a solution of at least one type of unsaturated fatty acid. The methods of the present invention further relate to the method of making a reagent comprising tissue factor reconstituted into preformed liposomes. The method of the present invention for making a tissue factor reagent comprises: providing tissue factor in solution; providing a solution of preformed liposomes comprising a mixture of phospholipids and at least one type of unsaturated fatty acid; and incubating the mixture.

    摘要翻译： 本申请涉及一种制备其中掺入膜蛋白的脂质体的方法，所述方法包括：在溶液中提供膜蛋白; 提供预先形成的脂质体的溶液; 并孵育混合物。 在提供预先形成的脂质体的溶液的步骤之前，通过将磷脂的混合物与至少一种类型的不饱和脂肪酸的溶液组合而形成脂质体。 本发明的方法还涉及制备包含重构成预先形成的脂质体的组织因子的试剂的方法。 本发明的制备组织因子试剂的方法包括：在溶液中提供组织因子; 提供包含磷脂和至少一种不饱和脂肪酸的混合物的预先形成的脂质体的溶液; 并孵育混合物。

    Random detection of antigens with antibodies immobilized on soluble submicron particles
    19.
    发明授权
    Random detection of antigens with antibodies immobilized on soluble submicron particles 失效
    用固定在可溶性亚微米颗粒上的抗体随机检测抗原

    公开(公告)号：US6121056A

    公开(公告)日：2000-09-19

    申请号：US226172

    申请日：1994-04-12

    申请人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

    发明人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

    IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/552

    CPC分类号： G01N33/552 ,  G01N33/54306 ,  G01N33/545 ,  Y10S436/823

    摘要： Methods, compositions and articles of manufacture are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. At least two dendrimer-reagent preparations with different analyte specificities may be immobilized on a solid phase. Alternatively, at least one dendrimer-reagent preparation having multiple specificities may be immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

    摘要翻译： 提供方法，组合物和制品用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在。 具有不同分析物特异性的至少两种树枝状大分子试剂制剂可以固定在固相上。 或者，至少一种具有多个特异性的树枝状大分子试剂制剂可固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

    Demountable heat sink
    20.
    发明授权
    Demountable heat sink 失效
    可拆卸散热器

    公开(公告)号：US5754400A

    公开(公告)日：1998-05-19

    申请号：US795049

    申请日：1997-02-05

    申请人： Sanjeev Balwant Sathe ,  Pratap Singh

    发明人： Sanjeev Balwant Sathe ,  Pratap Singh

    IPC分类号： H01L23/40 ,  H05K7/20

    CPC分类号： H01L23/4006 ,  H01L2224/73253

    摘要： A demountable, and thus re-usable, conductive heat sink for dissipation of excess heat of an electronic component. The heat sink uses a T-bolt and T-slot for attachment to the electronic component and the printed circuit board, respectively. This attachment eliminates the typical concerns associated with the placement of traces on the PCB to accommodate the holes placed therein for the attachment of conventional heat sinks. The improved designed also facilitates demounting of the heat sink from the printed circuit board and the electronic component without damage thereto.

    摘要翻译： 可拆卸且因此可重复使用的导电散热器，用于消散电子部件的多余热量。 散热器分别使用T型螺栓和T型槽连接到电子部件和印刷电路板。 该附件消除了与PCB上的迹线放置相关的典型问题，以容纳放置在其中以用于附接常规散热器的孔。 改进的设计还有助于散热器从印刷电路板和电子部件的拆卸而不损坏其。