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    Apparatus and method for extracting microbial cells
    11.
    发明授权
    Apparatus and method for extracting microbial cells 有权

    公开(公告)号：US10662420B2

    公开(公告)日：2020-05-26

    申请号：US15612091

    申请日：2017-06-02

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/24

    Abstract: Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

    Methods of targeted antibiotic susceptibility testing
    12.
    发明授权
    Methods of targeted antibiotic susceptibility testing 有权

    公开(公告)号：US10233483B2

    公开(公告)日：2019-03-19

    申请号：US14901200

    申请日：2014-07-03

    Applicant: Qvella Corporation

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/68 ,  C12Q1/689

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    Methods and compositions for the selective lysis of blood cells and separation of microbial cells
    14.
    发明授权
    Methods and compositions for the selective lysis of blood cells and separation of microbial cells 有权

    公开(公告)号：US12253513B2

    公开(公告)日：2025-03-18

    申请号：US17057088

    申请日：2019-05-24

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Vilcy Parmar ,  Sukhdev Manku ,  Alaleh Samiei

    IPC: G01N33/49 ,  G01N35/00

    Abstract: Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.

    Methods of performing nucleic acid stabilization and separation
    15.
    发明授权
    Methods of performing nucleic acid stabilization and separation 有权

    公开(公告)号：US11339366B2

    公开(公告)日：2022-05-24

    申请号：US16347658

    申请日：2017-11-08

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine

    IPC: C12Q1/68 ,  C07H21/00 ,  C12N1/06 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6876

    Abstract: Methods are provided for the stabilization and separation of nucleic acids from a sample via contact of the sample with a lysis and stabilization reagent that includes a cationic detergent. The cationic detergent lyses cells in the sample and stabilizes the released nucleic acids via the formation of nucleic acid-surfactant (NAS) complexes. The NAS complexes are centrifugally precipitated, washed, the resuspended in an aqueous resuspension liquid, forming a NAS complex suspension. The suspension is thermally processed to disintegrate the NAS complexes, thereby releasing the nucleic acids and forming a nucleic acid solution. In some example embodiments, the aqueous resuspension liquid is selected to be suitable for performing molecular amplification assays, such that the nucleic acid solution may be employed for performing a molecular amplification assay in the absence of further nucleic acid extraction. Examples are provided whereby the present methods are adapted for performing transcriptomic biomarker assays.

    Methods and devices for electrical sample preparation
    16.
    发明授权
    Methods and devices for electrical sample preparation 有权

    公开(公告)号：US10378043B2

    公开(公告)日：2019-08-13

    申请号：US15401291

    申请日：2017-01-09

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC: C12Q1/6806 ,  C12M1/42 ,  C12M1/00 ,  C12N1/06 ,  C12N1/08 ,  C12N13/00 ,  B01L3/00 ,  C12Q3/00 ,  B01L7/00 ,  C12Q1/686

    Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

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