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公开(公告)号：US20210255088A1
公开(公告)日：2021-08-19
申请号：US17068573
申请日：2020-10-12
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Bahram Jalali , Eric D. Diebold , Brandon Buckley
IPC: G01N15/14 , G01N33/537 , G01N21/64
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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公开(公告)号：US11009459B2
公开(公告)日：2021-05-18
申请号：US16233242
申请日：2018-12-27
Inventor： Sebastian Karpf , Bahram Jalali , Robert Huber
IPC: G01N21/64 , H01S3/067 , H01S3/00 , G02B21/00 , H01S3/083 , A61B5/00 , G01N21/00 , H01S3/13 , H01S5/14 , H01S3/16 , H01S5/50
Abstract: An apparatus and methods for high-speed non-linear spectrally encoded multi-photon imaging that are particularly suited for use in two photon fluorescence and fluorescence lifetime imaging. The system is capable of optical image compression and scale invariant digital zoom. A wavelength agile laser with digitally synthesized electro-optic modulation in a master oscillator-power amplifier configuration is combined with spectral encoding to eliminate the speed limitations of inertial scanning. The technique for fast two photon fluorescent imaging with simultaneous lifetime imaging independently detects the location, amplitude and lifetime of fluorescent emission by synthesizing a sequential excitation beam via digital electro-optic modulation of a quasi-CW swept source followed by time encoded detection. For fluorescent imaging, spectral and temporal mappings are employed separately, with quasi-CW spectral encoding used for pumping and time encoding for constructing the image at fluorescence wavelength.
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公开(公告)号：US10222316B2
公开(公告)日：2019-03-05
申请号：US16019323
申请日：2018-06-26
Applicant: The Regents of the University of California
Inventor： Bahram Jalali , Eric Diebold , Brandon Buckley
IPC: G01N21/64 , G01N15/14 , G01N33/537 , G01N15/10
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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公开(公告)号：US20180364146A1
公开(公告)日：2018-12-20
申请号：US16019323
申请日：2018-06-26
Applicant: The Regents of the University of California
Inventor： Bahram Jalali , Eric Diebold , Brandon Buckley
IPC: G01N15/14 , G01N21/64 , G01N33/537 , G01N15/10
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N21/64 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N33/537 , G01N2015/1006 , G01N2015/1477 , G01N2021/6421 , G01N2201/067
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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15.发明申请公开(公告)号：US20180246037A1
公开(公告)日：2018-08-30
申请号：US15961678
申请日：2018-04-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
CPC classification number: G01N21/6408 , G01N21/05 , G01N21/6458 , G01N21/6486 , G01N2021/6415 , G01N2021/6419 , G01N2201/06113 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B27/1006 , G02F1/11
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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Patent Agency Ranking
16.发明授权公开(公告)号：US09983132B2
公开(公告)日：2018-05-29
申请号：US15221065
申请日：2016-07-27
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
CPC classification number: G01N21/6408 , G01N21/05 , G01N21/6458 , G01N21/6486 , G01N2021/6415 , G01N2021/6419 , G01N2201/06113 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B27/1006 , G02F1/11
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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公开(公告)号：US09479192B2
公开(公告)日：2016-10-25
申请号：US14749626
申请日：2015-06-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Bahram Jalali , Mohammad H. Asghari
CPC classification number: H03M5/22 , H03M7/3059
Abstract: A method and apparatus for compression of digital and analog data utilizing an anamorphic spectrum transformation to warp an analog or digital signal to provide time-bandwidth compression after sampling is performed on the warped signal. The anamorphic spectrum transformation performs reallocating samples to assign more samples to fine features with high frequency waveform portions and fewer samples to coarse features with low frequency portions of the analog temporal signal, resulting in shorter record length and fewer number of samples.
Abstract translation: 使用变形频谱变换压缩数字和模拟数据以扭曲模拟或数字信号以在采样之后对翘曲信号执行采样之后的时间带宽压缩的方法和装置。 变形频谱变换执行重新分配样本，以将具有高频波形部分的精细特征和较少采样的样本分配给具有模拟时间信号的低频部分的粗略特征，导致更短的记录长度和更少的样本数量。
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公开(公告)号：US08686712B2
公开(公告)日：2014-04-01
申请号：US13855564
申请日：2013-04-02
Applicant: The Regents of the University of California
Inventor： Shalabh Gupta , Bahram Jalali , Ali Motafakker-Fard
IPC: G01R31/00
CPC classification number: G01R13/0218 , G02F1/225 , G02F7/00 , H03M1/1042 , H03M1/12
Abstract: A time-stretched enhanced recording scope (TiSER) is described using time stretch analog-to-digital conversion in a real-time burst mode. A chirped optical signal is modulated in response to receiving segments of an input signal. The optical signal with its modulated input signal, is stretched through an optical medium and digitized to represent the waveform segment. TiSER provides ultra-fast real-time sampling within short segment bursts of the original input signal while providing an ability to detect non-repetitive events. Methods and apparatus are also described for providing real-time information about inter-symbol information (ISI), rapidly determining bit-error rates (BER), performing time-domain reflectometry (TDR), generating eye diagrams for serial data, facilitating digital correction of data, clock recovery, optical carrier phase recovery, and otherwise increasing the speed and/or accuracy of a diverse range of high-speed signal measurement and processing activities.
Abstract translation: 使用实时突发模式中的时间拉伸模数转换来描述时间延长的增强记录范围（TiSER）。 响应于输入信号的接收段调制啁啾光信号。 具有调制输入信号的光信号通过光学介质被拉伸并数字化以表示波形段。 TiSER在原始输入信号的短片段突发中提供超快速实时采样，同时提供检测非重复事件的能力。 还描述了用于提供关于符号间信息（ISI），快速确定误码率（BER），执行时域反射计（TDR），生成串行数据眼图，促进数字校正的实时信息的方法和装置 的数据，时钟恢复，光载波相位恢复，否则增加了各种范围的高速信号测量和处理活动的速度和/或精度。
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19.发明申请公开(公告)号：US20250155371A1
公开(公告)日：2025-05-15
申请号：US19021025
申请日：2025-01-14
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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20.发明申请公开(公告)号：US20250020591A1
公开(公告)日：2025-01-16
申请号：US18818406
申请日：2024-08-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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