公开(公告)号：US11628436B2

公开(公告)日：2023-04-18

申请号：US16918661

申请日：2020-07-01

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Maung Kyaw Khaing Oo ,  Xudong Fan

IPC: B01L3/00

Abstract: An assay plate assembly comprising a plurality of microfluidic modules arranged in a rectilinear matrix of rows and columns microfluidic channels. Each microfluidic module has an inlet well leading to a serpentine microfluidic channel that is set at a cant angle. The well is laterally offset from the detection area to avoid optical interference. The geometric center of each detection area is positioned according to ANSI/SLAS standards for well-centers. A drain from each microfluidic channel is located so that it does not interfere with any detection areas. An array of micro-posts are disposed within each microfluidic channel. The micro-posts extend perpendicularly from the top surface of the top plate toward the underside and are equally distributed throughout the entire detection area. The plate assembly provides reduced assay time and sample volume, and increased sensitivity and specificity in biological and chemical assays.

公开(公告)号：US10267763B2

公开(公告)日：2019-04-23

申请号：US15037809

申请日：2014-12-04

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Zhaohui Zhong ,  Girish Kulkarni ,  Karthik Reddy ,  Xudong Fan

IPC: B01D53/02 ,  B82Y30/00 ,  G01N27/414

Abstract: An improved sensing method is provided for rapid analyte detection. The method includes: applying an AC excitation signal to the channel region of the transistor; applying an AC drive signal to the transistor; delivering an analyte of interest to a channel region of a transistor; and monitoring a mixing current of the excitation signal and the drive signal through the transistor, where a change in the mixing current is indicative of the concentration of the analyte of interest.

公开(公告)号：US20180095060A1

公开(公告)日：2018-04-05

申请号：US15724665

申请日：2017-10-04

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Jiwon Lee ,  Menglian Zhou ,  Hongbo Zhu ,  Katsuo Kurabayashi

IPC: G01N30/46

Abstract: The present disclosure provides a method for conducting comprehensive chromatography analysis. Broadly, the method comprises separating a sample in a first chromatographic column to generate a primary stream, which is directed toward a non-modulator switching system comprising at least one micro-switch and at least one valve. The non-modulator switching system is continuously operated to: (a) selectively direct a portion of the primary stream to one of a plurality of thermal injectors and accumulating the portion of the primary stream for a predetermined amount of time; (b) inject the portion of the primary stream into one of a plurality of secondary chromatographic columns; (c) detect one or more analytes in a secondary stream exiting the secondary chromatographic column; and repeat (a)-(c) to selectively direct other portions of the primary stream to other thermal injectors and secondary chromatographic columns until all of the analytes in the sample are detected.

公开(公告)号：US20140322729A1

公开(公告)日：2014-10-30

申请号：US14357151

申请日：2012-11-08

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Yunbo Guo ,  Maung Kyaw Khaing Oo

IPC: G01N33/543 ,  G01N21/65

CPC classification number: G01N33/54373 ,  G01N21/31 ,  G01N21/64 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/0346

Abstract: 3-dimensional surface-enhanced Raman scattering (SERS), as well as absorption/fluorescence/luminescence detection is carried out using a platform based on nanoparticle-functionalized flow-through multi-hole capillaries for rapid analyte detection. The configuration provides an increased active area and fluidic channels for efficient sample delivery, and also confines and transmits light for a large signal accumulation. Using a capillary consisting of thousands of micron-sized holes adsorbed with gold nanoparticles, a detection limit better than 100 fM is achieved.

Abstract translation: 使用基于纳米颗粒功能化的流通多孔毛细管的平台进行三维表面增强拉曼散射（SERS）以及吸收/荧光/发光检测，用于快速分析物检测。 该配置提供增加的有效面积和流体通道用于有效的样品输送，并且还限制和透射用于大信号积聚的光。 使用吸附有金纳米颗粒的数千微米尺寸的孔组成的毛细管，达到优于100fM的检测限。

公开(公告)号：US20130169970A1

公开(公告)日：2013-07-04

申请号：US13729983

申请日：2012-12-28

Applicant: The Regents of the University of Michigan

Inventor： Xudong Fan ,  Karthik Reddy ,  Yunbo Guo

IPC: G01J3/45

CPC classification number: G01J3/45 ,  G01N21/274 ,  G01N21/39 ,  G01N30/74

Abstract: A self-referencing composite Fabry-Pérot cavity sensor, including methods of use and manufacture. The cavity sensor comprises a substrate defining a first cavity portion juxtaposed to a second cavity portion. The first and second cavity portions are provided having a predetermined depth offset. A polymer or other dielectric material is disposed within the first and second cavity portions. An interference spectrum resulting from a light source of a known wavelength is reflected through the sensor and produces a first refractive index from the first cavity portion offset by a second refractive index from the second cavity portion. The difference in refractive indices can be used to determine various physical parameters. An optical sensor according to the present technology may be used with vapor sensing, pressure sensing, protein detection, photo-acoustic imaging, and the like.

Abstract translation: 自参考复合法布里 - 珀罗腔传感器，包括使用和制造方法。 空腔传感器包括限定与第二空腔部分并置的第一空腔部分的基板。 第一和第二空腔部分具有预定的深度偏移。 聚合物或其它介电材料设置在第一和第二空腔部分内。 由已知波长的光源产生的干涉光谱通过传感器反射，并从第一空腔部分产生偏离第二空腔部分的第二折射率的第一折射率。 折射率的差异可用于确定各种物理参数。 根据本技术的光学传感器可以与蒸气感测，压力感测，蛋白质检测，光声成像等一起使用。

公开(公告)号：US20200330987A1

公开(公告)日：2020-10-22

申请号：US16918661

申请日：2020-07-01

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Maung Kyaw Khaing Oo ,  Xudong Fan

IPC: B01L3/00

Abstract: An assay plate assembly comprising a plurality of microfluidic modules arranged in a rectilinear matrix of rows and columns microfluidic channels. Each microfluidic module has an inlet well leading to a serpentine microfluidic channel that is set at a cant angle. The well is laterally offset from the detection area to avoid optical interference. The geometric center of each detection area is positioned according to ANSI/SLAS standards for well-centers. A drain from each microfluidic channel is located so that it does not interfere with any detection areas. An array of micro-posts are disposed within each microfluidic channel. The micro-posts extend perpendicularly from the top surface of the top plate toward the underside and are equally distributed throughout the entire detection area. The plate assembly provides reduced assay time and sample volume, and increased sensitivity and specificity in biological and chemical assays.

公开(公告)号：US10768150B2

公开(公告)日：2020-09-08

申请号：US15724665

申请日：2017-10-04

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Jiwon Lee ,  Menglian Zhou ,  Hongbo Zhu ,  Katsuo Kurabayashi

IPC: G01N30/46 ,  G01N30/02

Abstract: The present disclosure provides a method for conducting comprehensive chromatography analysis. Broadly, the method comprises separating a sample in a first chromatographic column to generate a primary stream, which is directed toward a non-modulator switching system comprising at least one micro-switch and at least one valve. The non-modulator switching system is continuously operated to: (a) selectively direct a portion of the primary stream to one of a plurality of thermal injectors and accumulating the portion of the primary stream for a predetermined amount of time; (b) inject the portion of the primary stream into one of a plurality of secondary chromatographic columns; (c) detect one or more analytes in a secondary stream exiting the secondary chromatographic column; and repeat (a)-(c) to selectively direct other portions of the primary stream to other thermal injectors and secondary chromatographic columns until all of the analytes in the sample are detected.

公开(公告)号：US20180221876A1

公开(公告)日：2018-08-09

申请号：US15944962

申请日：2018-04-04

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Maung Kyaw Khaing Oo ,  Xudong Fan

IPC: B01L3/00

Abstract: An assay plate assembly comprising a plurality of microfluidic modules arranged in a rectilinear matrix of rows and columns microfluidic channels. Each microfluidic module has an inlet well leading to a serpentine microfluidic channel that is set at a cant angle. The well is laterally offset from the detection area to avoid optical interference. The geometric center of each detection area is positioned according to ANSI/SLAS standards for well-centers. A drain from each microfluidic channel is located so that it does not interfere with any detection areas. An array of optically-transmissive micro-posts are disposed within each microfluidic channel. The micro-posts extend perpendicularly from the top surface of the top plate toward the underside and are equally distributed throughout the entire detection area. The plate assembly provides reduced assay time and sample volume, and increased sensitivity and specificity in biological and chemical assays.

公开(公告)号：US09103727B2

公开(公告)日：2015-08-11

申请号：US13729983

申请日：2012-12-28

Applicant: The Regents of the University of Michigan

Inventor： Xudong Fan ,  Karthik Reddy ,  Yunbo Guo

IPC: G01B9/02 ,  G01J3/45 ,  G01N21/27 ,  G01N21/39 ,  G01N30/74

CPC classification number: G01J3/45 ,  G01N21/274 ,  G01N21/39 ,  G01N30/74

Abstract: A self-referencing composite Fabry-Pérot cavity sensor, including methods of use and manufacture. The cavity sensor comprises a substrate defining a first cavity portion juxtaposed to a second cavity portion. The first and second cavity portions are provided having a predetermined depth offset. A polymer or other dielectric material is disposed within the first and second cavity portions. An interference spectrum resulting from a light source of a known wavelength is reflected through the sensor and produces a first refractive index from the first cavity portion offset by a second refractive index from the second cavity portion. The difference in refractive indices can be used to determine various physical parameters. An optical sensor according to the present technology may be used with vapor sensing, pressure sensing, protein detection, photo-acoustic imaging, and the like.

Abstract translation: 自参考复合法布里 - 珀罗腔传感器，包括使用和制造方法。 空腔传感器包括限定与第二空腔部分并置的第一空腔部分的基板。 第一和第二空腔部分具有预定的深度偏移。 聚合物或其它介电材料设置在第一和第二空腔部分内。 由已知波长的光源产生的干涉光谱通过传感器反射，并从第一空腔部分产生偏离第二空腔部分的第二折射率的第一折射率。 折射率的差异可用于确定各种物理参数。 根据本技术的光学传感器可以与蒸气感测，压力感测，蛋白质检测，光声成像等一起使用。

公开(公告)号：US20140298990A1

公开(公告)日：2014-10-09

申请号：US14357157

申请日：2012-11-08

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Yogesh B. Gianchandani ,  Jing Liu

IPC: G01N30/46

CPC classification number: G01N30/461 ,  G01N30/46 ,  G01N30/463 ,  G01N30/465 ,  G01N30/466 ,  G01N30/468 ,  G01N30/6095 ,  G01N30/72

Abstract: The present disclosure provides adaptive methods for gas chromatography analysis of a gas sample comprising one or more target analytes (such as a micro-gas chromatography) and adaptive gas chromatography devices for carrying out such analytical methods. Broadly, the system can regulate flow into a downstream chromatographic column by detecting one or more upstream conditions. For example, one adaptive chromatography device comprises a first column, a modulator component, and a second column. A first detector or sensor detects the presence of target analytes upstream from the second column, while a second detector detects the presence of target analytes eluted from the second column. The modulator component assembly is responsive to an output generated by the first detector and adaptively regulates fluid flow into the second column. Such adaptive chromatography (micro-GC) systems have higher separation speed, better analyte identification capability, and far greater energy savings.

Abstract translation: 本公开提供了用于气体样品的气相色谱分析的自适应方法，所述气体样品包含一种或多种靶分析物（例如微气相色谱）和用于进行这种分析方法的适应性气相色谱装置。 通常，系统可以通过检测一个或多个上游条件来调节流入下游色谱柱的流量。 例如，一个自适应色谱装置包括第一列，调制器组件和第二列。 第一检测器或传感器检测来自第二列上游的目标分析物的存在，而第二检测器检测从第二列洗脱的目标分析物的存在。 调制器部件组件响应于由第一检测器产生的输出并且自适应地调节流入第二列的流体流。 这种自适应色谱（micro-GC）系统具有更高的分离速度，更好的分析物识别能力和更大的能量节省。