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41 条记录 (耗时 0.026 秒)

    (NUCLEIC ACID)-POLYSACCHARIDE COMPLEX
    14.
    发明申请
    (NUCLEIC ACID)-POLYSACCHARIDE COMPLEX 审中-公开
    （核酸） - 多糖复合物

    公开(公告)号：US20130267576A1

    公开(公告)日：2013-10-10

    申请号：US13810533

    申请日：2011-07-12

    申请人： Kazuo Sakurai

    发明人： Kazuo Sakurai

    IPC分类号： C12N15/113

    CPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K31/715 ,  A61K47/61 ,  C07H21/04 ,  C08B37/00 ,  C08B37/0024 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3517 ,  C12N2320/51 ,  C12N2320/53

    摘要： The present invention provides a nucleic acid-polysaccharide complex that is highly stable and has reduced cytotoxicity in a high yield. That is, the present invention provides a nucleic acid-polysaccharide complex of an antisense DNA having polydeoxyadenine (dA) as a polysaccharide binding site and a polysaccharide having a β-1,3-glucan skeleton wherein at least part of phosphodiester links of the polydeoxyadenine is phosphorothioated (provided that the phosphodiester links of the antisense DNA and the polydeoxyadenine are not entirely phosphorothioated).

    摘要翻译： 本发明提供高度稳定且以高产率降低的细胞毒性的核酸 - 多糖复合物。 也就是说，本发明提供了具有多脱氧腺嘌呤（dA）作为多糖结合位点的反义DNA和具有β-1,3-葡聚糖骨架的多糖的核酸 - 多糖复合物，其中多脱氧腺嘌呤的至少部分磷酸二酯连接 是硫代磷酸化的（条件是反义DNA和多脱氧腺嘌呤的磷酸二酯链不完全硫代磷酸化）。

    Gene carriers with the use of polysaccharide and process for producing the same
    16.
    发明授权
    Gene carriers with the use of polysaccharide and process for producing the same 有权
    使用多糖的基因载体及其生产方法

    公开(公告)号：US07521433B2

    公开(公告)日：2009-04-21

    申请号：US10471366

    申请日：2002-03-11

    申请人： Taro Kimura ,  Masami Mizu ,  Kazuo Sakurai ,  Seiji Shinkai ,  Kazuya Koumoto

    发明人： Taro Kimura ,  Masami Mizu ,  Kazuo Sakurai ,  Seiji Shinkai ,  Kazuya Koumoto

    IPC分类号： A61K31/716 ,  C07H15/04

    CPC分类号： A61K9/0019 ,  A61K31/711 ,  A61K31/716 ,  A61K47/36 ,  A61K48/0041 ,  C12N15/87

    摘要： Disclosed are gene carriers utilizing β-1,3-glucan and methods of preparing the same. The β-1,3-glucan has at least one 1,6-glucopyranoside branch and is chemically modified through periodate oxidation and reductive amination so as to impart nucleic acid-binding functional groups (for example, cationic functional groups) to at least some of the 1,6-glucopyranoside branches thereof. Triple helix β-1,3-glucan is dissolved in a polar organic solvent to form single-stranded β-1,3-glucan. By replacing, in the presence of a nucleic acid, the polar organic solvent for the solution containing the chemically modified single-stranded β-1,3-glucan by water, a complex (gene carrier) is formed in which the nucleic acid is bound to double-stranded β-1,3-glucan.

    摘要翻译： 公开了利用β-1,3-葡聚糖的基因载体及其制备方法。 β-1,3-葡聚糖具有至少一个1,6-吡喃葡萄糖苷分支，并通过高碘酸氧化和还原胺化进行化学修饰，从而将核酸结合官能团（例如阳离子官能团）赋予至少一些 的1,6-吡喃葡萄糖苷分枝。 将三螺旋β-1,3-葡聚糖溶于极性有机溶剂中以形成单链β-1,3-葡聚糖。 通过在核酸存在下用水代替含有化学修饰的单链β-1,3-葡聚糖的溶液的极性有机溶剂，形成核酸结合的复合物（基因载体） 到双链β-1,3-葡聚糖。

    Apparatus and method for wax tree coating and product manufacturing method using the apparatus and method
    17.
    发明授权
    Apparatus and method for wax tree coating and product manufacturing method using the apparatus and method 失效
    蜡树涂料的设备和方法以及使用该设备和方法的产品制造方法

    公开(公告)号：US5460217A

    公开(公告)日：1995-10-24

    申请号：US154440

    申请日：1993-11-19

    申请人： Kazuo Sakurai ,  Tosiaki Watanabe

    发明人： Kazuo Sakurai ,  Tosiaki Watanabe

    IPC分类号： B22C9/04 ,  B22C23/02 ,  B22C9/02 ,  B22C13/08

    CPC分类号： B22C9/04

    摘要： An apparatus and a method for manufacturing cast parts by means of a lost wax process which is fully automated and unmanned. The wax tree coating processes, ranging from after a wax tree manufacturing step to immediately before a dewaxing step comprises a handling device for handling wax trees, a pair of slurry fluid dipping baths disposed with the handling device in between, a pair of sanding fluidized bed tanks disposed with the handling device in between, and a pair of conveyors for suspending and transferring the wax trees for not less than given drying time, wherein the handling device chucks the wax tree suspended and transferred by the conveyor at a given transfer position by means of a clamp unit of the handling device, moves the wax tree to the slurry fluid dipping bath and then to the sanding fluidized bed tank to coat the wax tree with a slurry fluid and sand, and returns the coated wax tree to the hanger conveyor again, with the sequence of operations automatically controlled by a controller.

    摘要翻译： 一种通过完全自动化和无人化的失蜡工艺制造铸件的设备和方法。 从蜡蜡制造步骤之后到脱蜡步骤之前的蜡树涂覆方法包括用于处理蜡树的处理装置，在处理装置之间设置的一对浆液流体浸渍浴，一对砂磨流化床 设置有处理装置的箱体和一对用于在不低于给定干燥时间的情况下悬挂和转移蜡树的输送机，其中处理装置将通过输送机悬挂和传送的蜡树在一个给定的转移位置 将处理装置的夹持单元移动到浆液流体浸渍浴中，然后到达砂浆流化床槽以用浆液和砂涂覆蜡树，并将涂覆的蜡树再次返回到衣架输送机 ，其操作顺序由控制器自动控制。

    Gene carrier
    19.
    发明授权
    Gene carrier 有权
    基因载体

    公开(公告)号：US08017742B2

    公开(公告)日：2011-09-13

    申请号：US10465402

    申请日：2003-06-19

    申请人： Kazuo Sakurai ,  Seiji Shinkai ,  Taro Kimura ,  Kengo Tabata ,  Kazuya Koumoto ,  Oliver Gronwald

    发明人： Kazuo Sakurai ,  Seiji Shinkai ,  Taro Kimura ,  Kengo Tabata ,  Kazuya Koumoto ,  Oliver Gronwald

    IPC分类号： C07H1/00 ,  C07H21/44

    CPC分类号： C07H15/04 ,  A61K47/61 ,  A61K47/645 ,  A61K48/00 ,  C07B2200/11 ,  C07H21/00 ,  C08B37/0024 ,  C12N15/87

    摘要： Disclosed is a gene carrier composed of a hydrogen-bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain, in which the polymer chain has a conformation similar to the conformation of a nucleic acid, and particularly, the polymer chain has helix parameters similar to the helix parameters of a nucleic acid of a helix structure. In a preferred embodiment, the hydrogen-bonding polymer is composed of β-1,3-glucan such as schizophyllan. The carrier can be expected to be applied as a vector and a separator for nucleic acids because it is capable of interacting with a nucleic acid to form a complex with the nucleic acid and thus carrying the nucleic acid.

    摘要翻译： 公开了一种基因载体，其由具有在聚合物链上形成的氢键位置的聚合物链的氢键聚合物组成，其中聚合物链具有与核酸构象相似的构象，特别是聚合物链 具有类似于螺旋结构的核酸的螺旋参数的螺旋参数。 在一个优选的实施方案中，氢键聚合物由诸如裂枝菌碱等的β-葡聚糖组成。 可以期望载体作为核酸的载体和分离子应用，因为它能够与核酸相互作用以与核酸形成复合物，从而携带核酸。

    Microphone Apparatus
    20.
    发明申请
    Microphone Apparatus 有权
    麦克风设备

    公开(公告)号：US20090046882A1

    公开(公告)日：2009-02-19

    申请号：US12083909

    申请日：2007-07-04

    申请人： Kazuo Sakurai ,  Masayuki Shimada

    发明人： Kazuo Sakurai ,  Masayuki Shimada

    IPC分类号： H04R19/04

    CPC分类号： H04R1/083 ,  H04R2410/07

    摘要： A microphone unit (1) has a first vibration plate. A support member (6) supports the microphone unit (1). A second vibration plate (5) is fixed to the support member (6) at a predetermined distance from the first vibration plate. An armoring body (2) covers the microphone unit (1), the support member (6) and the second vibration plate (5). A space surrounded by the support member (6), the first vibration plate and the second vibration plate (5) is a closed space (S1) with air kept therein.

    摘要翻译： 麦克风单元（1）具有第一振动板。 支撑构件（6）支撑麦克风单元（1）。 第二振动板（5）以与第一振动板预定的距离固定到支撑构件（6）。 铠装体（2）覆盖麦克风单元（1），支撑构件（6）和第二振动板（5）。 由支撑构件（6），第一振动板和第二振动板（5）包围的空间是其中保持有空气的封闭空间（S1）。