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34 条记录 (耗时 0.026 秒)

    Phycobiliprotein fluorescent conjugates
    15.
    发明授权
    Phycobiliprotein fluorescent conjugates 失效
    藻胆蛋白荧光共轭物

    公开(公告)号：US4542104A

    公开(公告)日：1985-09-17

    申请号：US483006

    申请日：1983-04-06

    申请人： Lubert Stryer ,  Alexander N. Glazer

    发明人： Lubert Stryer ,  Alexander N. Glazer

    IPC分类号： G01N33/533 ,  G01N33/542 ,  G01N33/52

    CPC分类号： G01N33/542 ,  G01N33/533 ,  Y10S436/80

    摘要： Fluorescent conjugates are employed providing combinations of a fluorescent sensitizer and a fluorescent phycobiliprotein. The conjugates find use in applications where large Stokes shifts, high absorption coefficients and high fluorescence quantum yields are desired. Particularly, combinations of phycobiliproteins are employed where the wavelength of excitation may be 50 nm or more different from the emission wavelength.

    摘要翻译： 使用荧光共轭物提供荧光增感剂和荧光藻胆蛋白的组合。 共轭物可用于需要大斯托克斯位移，高吸收系数和高荧光量子产率的应用中。 特别地，使用藻红蛋白的组合，其中激发波长可以与发射波长不同50nm或更大。

    Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display
    16.
    发明授权
    Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 失效
    多功能重组藻胆蛋白基荧光结构和phycobilisome显示

    公开(公告)号：US06649376B1

    公开(公告)日：2003-11-18

    申请号：US09469194

    申请日：1999-12-21

    申请人： Alexander N. Glazer ,  Yuping Cai

    发明人： Alexander N. Glazer ,  Yuping Cai

    IPC分类号： C12N506

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  Y10S435/822

    摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.

    摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了用于在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物，包括融合蛋白，其包含功能性显示结构域和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。

    Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
    17.
    发明授权
    Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation 失效
    通过荧光共振能量转移标记产生检测核苷酸序列变异

    公开(公告)号：US06573047B1

    公开(公告)日：2003-06-03

    申请号：US09547292

    申请日：2000-04-11

    申请人： Su-Chun Hung ,  Alexander N. Glazer ,  Richard A. Mathies

    发明人： Su-Chun Hung ,  Alexander N. Glazer ,  Richard A. Mathies

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6858 ,  C12Q2565/101 ,  C12Q2535/125

    摘要： The present invention provides methods and kits for identifying nucleotides at a variant site in a target molecule by forming fluorescence resonance energy transfer labeled product. The location and type of fluorescent labels in the labeled product provide strong signals and relatively high spectral purity that facilitate detection. Utilizing various secondary labels and different combinations of acceptor and donor labels, certain methods permit multiple analyses to be conducted simultaneously and at high throughput. The methods can be used in a variety of applications such as analyzing point mutations and single nucleotide polymorphisms (SNPs). In addition, the methods have utility in other applications in which specific sequence information is of value, including detection of pathogens, paternity disputes, prenatal testing and forensic analysis.

    摘要翻译： 本发明提供用于通过形成荧光共振能量转移标记产物来鉴定靶分子中变体位点处的核苷酸的方法和试剂盒。 标记产品中荧光标记的位置和类型提供强信号和较高的光谱纯度，便于检测。 利用各种二级标签和受体和供体标签的不同组合，某些方法允许同时进行多次分析并以高产量进行。 该方法可用于各种应用，如分析点突变和单核苷酸多态性（SNPs）。 此外，这些方法在其他应用中具有实用价值，其中具体的序列信息是有价值的，包括病原体的检测，亲子鉴定，产前检测和法医分析。

    Detection of specific sequences in nucleic acids
    19.
    发明授权
    Detection of specific sequences in nucleic acids 失效
    检测核酸中的特定序列

    公开(公告)号：US5962223A

    公开(公告)日：1999-10-05

    申请号：US574826

    申请日：1995-12-19

    申请人： Norman M. Whiteley ,  Michael W. Hunkapiller ,  Alexander N. Glazer

    发明人： Norman M. Whiteley ,  Michael W. Hunkapiller ,  Alexander N. Glazer

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6862 ,  C12Q1/6813 ,  C12Q1/6827 ,  Y10S435/803 ,  Y10S435/81

    摘要： The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook. In both instances, the presence of both the diagnostic probe and the contiguous probe is required for the label to appear in the assay. The above method is then applied to the detection of genetic diseases.

    摘要翻译： 本发明提供了一种用于诊断遗传异常或其他遗传病症的方法，其可以容易地自动化。 该方法用于确定变性核酸样品中靶序列的存在或不存在，并且需要将样品与与靶序列（诊断探针）的诊断部分互补的探针杂交，并且与探针互补 在诊断探针保持基本上仅与包含靶序列的样品核酸结合的条件下，与诊断部分（连续探针）连续的核苷酸序列。 然后将诊断探针和连续探针共价连接以产生与靶序列互补的靶探针，并且除去未连接的探针。 在优选模式中，其中一个探针被标记，使得靶序列的存在或不存在可以通过熔化样品核酸 - 靶标探针双链体，洗脱解离的靶标探针和测试标签来测试。 在另一个实施方案中，通过将钩连接到未标记的探针上，而无需首先除去未共价连接的探针，从而可以通过捕捉钩来回收标记的靶探针而进行测试。 在这两种情况下，诊断探针和连续探针的存在是标签出现在测定中所必需的。 然后将上述方法应用于遗传疾病的检测。

    Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
    20.
    发明授权
    Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores 失效
    二聚荧光能量转移染料包括不对称花青唑 - 吲哚宁发色团

    公开(公告)号：US5929227A

    公开(公告)日：1999-07-27

    申请号：US965913

    申请日：1997-11-07

    申请人： Alexander N. Glazer ,  Scott C. Benson

    发明人： Alexander N. Glazer ,  Scott C. Benson

    IPC分类号： G01N27/447 ,  C07H21/02 ,  C07H21/04

    CPC分类号： G01N27/44721

    摘要： Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.

    摘要翻译： 提供新型荧光异二聚体DNA染色能量转移染料，其结合不对称花青唑 - 吲哚宁染料，其提供强的DNA亲和力，大的斯托克斯位移和在红色区域中的发射。 这些染料特别适用于凝胶电泳和可能在多种情况下与各种组合物结合的标记物。 提供了试剂盒和各种化合物，其中试剂盒可用于同时检测各种部分，特别是使用单个窄波长照射源。 单个化合物的特征在于由于优化分离两个发色团的连接基团的长度而导致高的供体猝灭和对dsDNA的高亲和力。