-
公开(公告)号：US5521065A
公开(公告)日：1996-05-28
申请号：US257219
申请日：1994-06-08
CPC分类号： C12Q1/6862 , C12Q1/6813 , C12Q1/6827 , Y10S435/803 , Y10S435/81
摘要： A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments is disclosed. The method involves reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions. Following hybridization, any of the first and second probes hybridized to contiguous first and second target regions are ligated, and the sample is tested for the presence of expected probe ligation product. The presence of ligated product indicates that the target sequence is present in the sample.
摘要翻译： 公开了在单链核酸片段的混合物中测试靶序列的存在或不存在的方法。 该方法包括使单链核酸片段的混合物与与靶序列的第一区域互补的第一探针和与靶序列的第二区域互补的第二探针反应，其中第一和 在杂交条件下，第二目标区域彼此连续，其中两个探针稳定地与其相关联的靶区域杂交。 杂交后，将与连续的第一和第二靶区域杂交的任何第一和第二探针连接，并测试样品中是否存在预期的探针连接产物。 连接产物的存在表明靶序列存在于样品中。
-
公开(公告)号：US5962223A
公开(公告)日：1999-10-05
申请号：US574826
申请日：1995-12-19
CPC分类号： C12Q1/6862 , C12Q1/6813 , C12Q1/6827 , Y10S435/803 , Y10S435/81
摘要： The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook. In both instances, the presence of both the diagnostic probe and the contiguous probe is required for the label to appear in the assay. The above method is then applied to the detection of genetic diseases.
摘要翻译： 本发明提供了一种用于诊断遗传异常或其他遗传病症的方法，其可以容易地自动化。 该方法用于确定变性核酸样品中靶序列的存在或不存在，并且需要将样品与与靶序列（诊断探针）的诊断部分互补的探针杂交，并且与探针互补 在诊断探针保持基本上仅与包含靶序列的样品核酸结合的条件下，与诊断部分（连续探针）连续的核苷酸序列。 然后将诊断探针和连续探针共价连接以产生与靶序列互补的靶探针，并且除去未连接的探针。 在优选模式中，其中一个探针被标记，使得靶序列的存在或不存在可以通过熔化样品核酸 - 靶标探针双链体，洗脱解离的靶标探针和测试标签来测试。 在另一个实施方案中，通过将钩连接到未标记的探针上，而无需首先除去未共价连接的探针，从而可以通过捕捉钩来回收标记的靶探针而进行测试。 在这两种情况下，诊断探针和连续探针的存在是标签出现在测定中所必需的。 然后将上述方法应用于遗传疾病的检测。
-
公开(公告)号：US5242794A
公开(公告)日：1993-09-07
申请号：US361407
申请日：1989-06-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6862 , C12Q1/6813 , C12Q1/6827 , Y10S435/803 , Y10S435/81
摘要： The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook. In both instances, the presence of both the diagnostic probe and the contiguous probe is required for the label to appear in the assay. The above method is then applied to the detection of genetic diseases.
摘要翻译： 本发明提供了一种用于诊断遗传异常或其他遗传病症的方法，其可以容易地自动化。 该方法用于确定变性核酸样品中靶序列的存在或不存在，并且需要将样品与与靶序列（诊断探针）的诊断部分互补的探针杂交，并且与探针互补 在诊断探针保持基本上仅与包含靶序列的样品核酸结合的条件下，与诊断部分（连续探针）连续的核苷酸序列。 然后将诊断探针和连续探针共价连接以产生与靶序列互补的靶探针，并且除去未连接的探针。 在优选模式中，其中一个探针被标记，使得靶序列的存在或不存在可以通过熔化样品核酸 - 靶标探针双链体，洗脱解离的靶标探针和测试标签来测试。 在另一个实施方案中，通过将钩连接到未标记的探针上，而无需首先除去未共价连接的探针，从而可以通过捕捉钩来回收标记的靶探针而进行测试。 在这两种情况下，诊断探针和连续探针的存在是标记出现在测定中所必需的。 然后将上述方法应用于遗传疾病的检测。
-
公开(公告)号：US4883750A
公开(公告)日：1989-11-28
申请号：US681055
申请日：1984-12-13
CPC分类号： C12Q1/6862 , C12Q1/6816 , C12Q1/6827 , C12Q2600/156 , Y10S435/803 , Y10S436/811
摘要： The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook. In both instances, the presence of both the diagnostic probe and the contiguous probe is required for the label to appear in the assay. The above method is then applied to the detection of genetic diseases.
摘要翻译： 本发明提供了一种用于诊断遗传异常或其他遗传病症的方法，其可以容易地自动化。 该方法用于确定变性核酸样品中靶序列的存在或不存在，并且需要将样品与与靶序列（诊断探针）的诊断部分互补的探针杂交，并且与探针互补 在诊断探针保持基本上仅与包含靶序列的样品核酸结合的条件下，与诊断部分（连续探针）连续的核苷酸序列。 然后将诊断探针和连续探针共价连接以产生与靶序列互补的靶探针，并且除去未连接的探针。 在优选模式中，其中一个探针被标记，使得靶序列的存在或不存在可以通过熔化样品核酸 - 靶标探针双链体，洗脱解离的靶标探针和测试标签来测试。 在另一个实施方案中，通过将钩连接到未标记的探针上，而无需首先除去未共价连接的探针，从而可以通过捕捉钩来回收标记的靶探针而进行测试。 在这两种情况下，诊断探针和连续探针的存在是标签出现在测定中所必需的。 然后将上述方法应用于遗传疾病的检测。
-
5.发明授权Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 有权多功能重组藻胆蛋白基荧光结构和phycobilisome显示公开(公告)号：US07176000B2
公开(公告)日：2007-02-13
申请号：US10617012
申请日：2003-07-10
申请人： Alexander N. Glazer , Yuping Cai
发明人： Alexander N. Glazer , Yuping Cai
CPC分类号： C07K14/415 , C07K2319/00 , Y10S435/822
摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein. including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物。 包括包含功能性显示结构域的融合蛋白和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。
-
6.发明授权Engineering of living cells for the expression of holo-phycobiliprotein-based constructs 有权用于表达全脂蛋白脂蛋白的构建体的活细胞工程公开(公告)号：US06740507B2
公开(公告)日：2004-05-25
申请号：US09919486
申请日：2001-07-31
申请人： Alexander N. Glazer , Aaron J. Tooley , Yuping Cai
发明人： Alexander N. Glazer , Aaron J. Tooley , Yuping Cai
IPC分类号： C12P2104
CPC分类号： C12N9/0095 , C07K14/43595 , C07K2319/21 , C07K2319/60 , C12N9/88 , C12N15/52 , C12N15/62
摘要： Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
摘要翻译： 描述表达荧光全血蓝蛋白融合蛋白的重组细胞及其使用方法。 所述细胞包含bilin，重组bilin还原酶，包含相应的载脂蛋白结构域的融合蛋白的载脂蛋白 - 磷脂蛋白融合蛋白前体，以及组分反应形成全血蓝蛋白融合蛋白的重组体藻胆蛋白结构域 - 胆固醇裂解酶 。 还描述了基于全藻蛋白脂蛋白的转录报道细胞和测定法，其中细胞条件地表达异源细胞，荧光，第一全息型蛋白脂蛋白结构域。
-
7.发明授权Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores 失效使用带有花青染料作为供体发色团的能量转移标记进行测序和检测的方法
公开(公告)号：US6150107A
公开(公告)日：2000-11-21
申请号：US164800
申请日：1998-10-01
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译： 能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。 花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。
-
8.发明授权DNA complexes with dyes designed for energy transfer as fluorescent markers 失效DNA复合物与设计用于能量转移的染料作为荧光标记物
公开(公告)号：US5401847A
公开(公告)日：1995-03-28
申请号：US9704
申请日：1993-01-27
IPC分类号： G01N33/58 , C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C07H21/04 , C09B23/04 , C12N15/09 , C12Q1/68 , G01N27/447 , C07H19/00 , G01N27/26
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , C12Q1/6818 , G01N27/44726 , G01N27/44747
摘要： Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.
摘要翻译： 提供了异源多聚体荧光团用于结合DNA，其允许在电分离中检测DNA并制备具有高荧光效率和大的斯托克斯位移的探针。 此外，通过适当选择荧光分子，可以使用单个窄波长带激发光源，同时获得具有足够分离的荧光发射以易于鉴别。
-
9.发明授权公开(公告)号：US06428667B1
公开(公告)日：2002-08-06
申请号：US09686147
申请日：2000-10-10
IPC分类号： B01D5702
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
-
10.发明授权Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白公开(公告)号：US06391590B1
公开(公告)日：2002-05-21
申请号：US07780717
申请日：1991-10-21
IPC分类号： C12N1562
CPC分类号： C07H21/04 , C07K14/36 , C07K14/825 , C07K2319/00 , C07K2319/22
摘要： Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
摘要翻译： 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。
-
-
-
-
-
-
-
-
-
搜索结果
34 条记录 (耗时 0.022 秒)
