公开(公告)号：US20160264612A1

公开(公告)日：2016-09-15

申请号：US15167917

申请日：2016-05-27

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/68

CPC分类号： C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20140206553A1

公开(公告)日：2014-07-24

申请号：US13959660

申请日：2013-08-05

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68

CPC分类号： C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

摘要翻译： 本发明提供了通过在聚合酶反应中将核苷酸类似物掺入DNA的生长链中之后​​检测核苷酸类似物的同一性来提供将核酸连接到固体表面和测序核酸的方法。 本发明还提供核苷酸类似物，其包含通过可切割连接子连接至核苷酸类似物的独特标记，以及可脱氧化学基团以封端脱氧核糖3'-位上的-OH基团。

公开(公告)号：US10662472B2

公开(公告)日：2020-05-26

申请号：US16200557

申请日：2018-11-26

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68 ,  C07H19/10 ,  C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6872 ,  C12Q1/6876 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US10648028B2

公开(公告)日：2020-05-12

申请号：US16200549

申请日：2018-11-26

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68 ,  C07H19/10 ,  C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6872 ,  C12Q1/6876 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US10428380B2

公开(公告)日：2019-10-01

申请号：US16150191

申请日：2018-10-02

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68 ,  C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6872 ,  C07H19/10 ,  C12Q1/6876 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20180201642A1

公开(公告)日：2018-07-19

申请号：US15915983

申请日：2018-03-08

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6872 ,  C12Q1/68 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6874

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US09718852B2

公开(公告)日：2017-08-01

申请号：US15380270

申请日：2016-12-15

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/68

CPC分类号： C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20170088574A1

公开(公告)日：2017-03-30

申请号：US15380284

申请日：2016-12-15

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/10 ,  C12Q1/68

CPC分类号： C07H19/10 ,  C07B2200/11 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2563/107 ,  C12Q2535/101 ,  C12Q2525/186 ,  C12Q2535/122

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20150197800A1

公开(公告)日：2015-07-16

申请号：US14670748

申请日：2015-03-27

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

摘要翻译： 本发明提供了通过在聚合酶反应中将核苷酸类似物掺入DNA的生长链中之后​​检测核苷酸类似物的同一性来提供将核酸连接到固体表面和测序核酸的方法。 本发明还提供核苷酸类似物，其包含通过可切割连接子连接至核苷酸类似物的独特标记，以及可脱氧化学基团以封端脱氧核糖3'-位上的-OH基团。

公开(公告)号：US10669577B2

公开(公告)日：2020-06-02

申请号：US14670748

申请日：2015-03-27

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C12Q1/68 ,  C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6872 ,  C07H19/10 ,  C12Q1/6876 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.