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公开(公告)号：US20230125093A1
公开(公告)日：2023-04-27
申请号：US17970874
申请日：2022-10-21
Applicant: Waters Technologies Corporation
Inventor： Matthew A. Lauber , Kevin Wyndham , Szabolcs Fekete , Hua Yang , Stephan Koza
IPC: C12N15/10
Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.
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公开(公告)号：US20220091076A1
公开(公告)日：2022-03-24
申请号：US17484157
申请日：2021-09-24
Applicant: Waters Technologies Corporation
Inventor： Mathew DeLano , Matthew A. Lauber , Kevin Wyndham , Jennifer M. Nguyen
Abstract: The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The coating is a vapor deposited inorganic-organic hybrid coating, such as a vapor deposited C2 coating.
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13.发明申请公开(公告)号：US20210223217A1
公开(公告)日：2021-07-22
申请号：US17204021
申请日：2021-03-17
Applicant: Waters Technologies Corporation
Inventor： Stephen J. Shiner , Cheryl Boissel , Matthew A. Lauber , Kevin Wyndham , Michael O. Fogwill
Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of a vapor deposition coated frit together with an uncoated frit in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having a single coated frit within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.
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公开(公告)号：US20250130232A1
公开(公告)日：2025-04-24
申请号：US19005819
申请日：2024-12-30
Applicant: Waters Technologies Corporation
Inventor： Kevin Wyndham , Beatrice Muriithi , Martin Gilar , Yeliz Tunc Sarisozen
IPC: G01N33/569 , G01N33/53 , G01N33/543 , G01N33/74
Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).
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15.发明授权公开(公告)号：US12259368B2
公开(公告)日：2025-03-25
申请号：US17471393
申请日：2021-09-10
Applicant: Waters Technologies Corporation
Inventor： Amit Patel , Moon Chul Jung , Matthew A. Lauber , Kevin Wyndham , Mathew H. DeLano , Jennifer Simeone
Abstract: The present disclosure is directed to methods of characterizing a system containing a chromatographic column. The methods can include introducing a sample comprising a positive control and a negative control to the system containing a chromatographic column, wherein the positive control is a sensitive probe that interacts with the system and the negative control is substantially non-interacting with the system; after passing the sample through the chromatographic column, detecting the positive control and the negative control; and determining system suitability by comparing the amount of detected positive control to negative control. In some embodiments, determining system suitability (e.g., inertness of sample to the system) is accomplished by determining a ratio of detected positive control to negative control.
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Patent Agency Ranking
16.发明授权公开(公告)号：US12181452B2
公开(公告)日：2024-12-31
申请号：US17204021
申请日：2021-03-17
Applicant: Waters Technologies Corporation
Inventor： Stephen J. Shiner , Cheryl Boissel , Matthew A. Lauber , Kevin Wyndham , Michael O. Fogwill
Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of a vapor deposition coated frit together with an uncoated frit in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having a single coated frit within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.
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公开(公告)号：US20230073279A1
公开(公告)日：2023-03-09
申请号：US17896324
申请日：2022-08-26
Applicant: Waters Technologies Corporation
Inventor： MingCheng Xu , Darryl W. Brousmiche , Kevin Wyndham , Daniel P. Walsh , Nicole L. Lawrence
IPC: B01J20/26 , B01J20/28 , B01J20/285
Abstract: In various embodiments, the present disclosure pertains to organic polymer core-shell particles that comprise a non-porous organic polymer core (i.e., having a pore volume of less than 0.1 cc/g) and a porous organic polymer shell (i.e., having a pore volume of greater than 0.1 cc/g), in which the porous organic polymer shell has a pore size ranging from 100 Å to 3000 Å. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such organic polymer core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such organic polymer core-shell particles and (b) flowing a mobile phase through the column.
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公开(公告)号：US20220203329A1
公开(公告)日：2022-06-30
申请号：US17688367
申请日：2022-03-07
Applicant: Waters Technologies Corporation
Inventor： Kevin Wyndham
IPC: B01J20/286
Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.
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19.发明申请公开(公告)号：US20210220814A1
公开(公告)日：2021-07-22
申请号：US17221471
申请日：2021-04-02
Applicant: Waters Technologies Corporation
Inventor： Mingcheng Xu , Darryl W. Brousmiche , Kevin Wyndham
IPC: B01J43/00 , B01J20/28 , B01J20/285 , B01J39/20 , B01J41/14 , B01J20/26 , B01D15/08 , C08F226/06
Abstract: The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the porous materials comprise a copolymer of a least one hydrophobic monomer and at least one hydrophilic monomer, wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 Å, wherein said material has a median pore diameter of about 100 Å to about 1000 Å, or both. In some embodiments, the at least one hydrophilic monomer has a log P value of less than 0.5. In some embodiments, the at least one hydrophilic monomer is selected from 4-acryloymorphine, N-(3-methoxypropyl)acrylamide, N,N′-methylenebis(acrylamide), acrylonitrile, ethylene glycol dimethacrylate, methyl acrylate, 4-acetoxystyrene, 4-vinyl pyridine, or a boronic-acid-containing monomer, among others.
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公开(公告)号：US20250052727A1
公开(公告)日：2025-02-13
申请号：US18933343
申请日：2024-10-31
Applicant: Waters Technologies Corporation
Inventor： Edouard S. P. Bouvier , Kevin Wyndham , Thomas H. Walter , Uwe D. Neue
IPC: G01N30/02 , B01D15/34 , B01J20/28 , B01J20/282 , B01J20/286 , G01N21/53
Abstract: The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles.
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