利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

92 条记录 (耗时 0.039 秒)

    Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same
    11.
    发明授权
    Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same 有权
    具有烃生产能力的微生物变体及使用其生产烃的方法

    公开(公告)号：US08895276B2

    公开(公告)日：2014-11-25

    申请号：US13702920

    申请日：2011-06-10

    申请人： Sang Yup Lee ,  Yong Jun Choi

    发明人： Sang Yup Lee ,  Yong Jun Choi

    IPC分类号： C12P5/00 ,  C12N9/02 ,  C12N9/18 ,  C12N9/88 ,  C12N9/00 ,  C12P5/02 ,  C12N15/52 ,  C12N15/70 ,  C07K14/245

    CPC分类号： C12P5/00 ,  C07K14/245 ,  C12N9/001 ,  C12N9/18 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12P5/005 ,  C12P5/02 ,  C12P5/026

    摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.

    摘要翻译： 本发明涉及具有生产碳氢化合物（包括烷烃）的能力的微生物变异体，以及使用该方法生产烃类的方法，包括使用烷烃的烷烃，更具体地说，涉及通过引入编码转化脂肪酶的基因而获得的微生物变体 对游离脂肪酸的酰基-Acp，编码将游离脂肪酸转化为脂肪酰辅酶A的酶的基因，编码将脂肪酰基-CoA转化为脂肪醛的酶的基因和编码将脂肪醛转化为烷烃的酶的基因转化为微生物 改进以适于生产包括烷烃在内的烃类，以及使用其制备包括烷烃在内的烃类的方法。 本发明的微生物变体具有很高的潜力用于通过附加的代谢通量工程来改善菌株，因此可用于包括烷烃在内的烃的工业生产。

    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME
    13.
    发明申请
    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
    具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法

    公开(公告)号：US20130017588A1

    公开(公告)日：2013-01-17

    申请号：US13497743

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    IPC分类号： C12N1/21 ,  C12P7/16 ,  C12N15/01

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

    摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

    摘要翻译： 本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。 更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。 在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。 通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

    RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME
    14.
    发明申请
    RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME 有权
    具有增加生产酒精的能力的重组突变体微生物和使用该醇的生产醇的方法

    公开(公告)号：US20120301936A1

    公开(公告)日：2012-11-29

    申请号：US13497736

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Joung Min Lee ,  Jung Ae Im ,  Hyohak Song

    IPC分类号： C12P7/16 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C12N9/13 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/74 ,  C12P3/00 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  Y02E50/10 ,  Y02E50/17

    摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.

    摘要翻译： 本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以 作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。 生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物 。 由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。 因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

    Method for fabricating a biochip using the high density carbon nanotube film or pattern
    15.
    发明授权
    Method for fabricating a biochip using the high density carbon nanotube film or pattern 有权
    使用高密度碳纳米管膜或图案制造生物芯片的方法

    公开(公告)号：US08067341B2

    公开(公告)日：2011-11-29

    申请号：US10805044

    申请日：2004-03-19

    申请人： Hee Tae Jung ,  Sang Yup Lee ,  Dae Hwan Jung ,  Byung Hun Kim ,  Young Koan Ko

    发明人： Hee Tae Jung ,  Sang Yup Lee ,  Dae Hwan Jung ,  Byung Hun Kim ,  Young Koan Ko

    IPC分类号： C40B60/00

    CPC分类号： G01N33/54353 ,  B82Y30/00 ,  G01N33/551 ,  Y10S977/742

    摘要： Carbon nanotube (CNT) films, patterns and biochips and methods of making the same are provided. Such a biochip comprises a bio-receptor attached by means of an exposed chemical functional group on a surface of a high density CNT film or pattern produced by repeated lamination of CNTs on a substrate with exposed amine groups. Various types of CNT-biochips may be fabricated by bonding of bio-receptors to a CNT pattern (or film) containing exposed carboxyl groups or modified by various chemical functional groups. Further, the CNT-biochip may be used to measure an electrical or electrochemical signal using both conductor and semiconductor properties of the CNT, thereby not needing labeling. Upon fluorescent measurement of DNA hybridization using such a CNT-DNA chip it is possible to show more distinct signals useful for genotyping, mutation detection, pathogen identification and the like.

    摘要翻译： 提供了碳纳米管（CNT）膜，图案和生物芯片及其制造方法。 这样的生物芯片包括通过暴露的化学官能团在高密度CNT膜的表面上附着的生物受体或通过在具有暴露的胺基的基底上重复层压CNT而产生的图案。 可以通过将生物受体结合到包含暴露的羧基或被各种化学官能团修饰的CNT图案（或膜）来制造各种类型的CNT-生物芯片。 此外，CNT-生物芯片可以用于使用CNT的导体和半导体性质来测量电或电化学信号，从而不需要标记。 在使用这种CNT-DNA芯片进行DNA杂交的荧光测量时，可能显示更有用于基因分型，突变检测，病原体鉴定等的不同信号。

    Method for whole surrounding surface display of target proteins using bacterial exosporium
    16.
    发明授权
    Method for whole surrounding surface display of target proteins using bacterial exosporium 失效
    使用细菌外植体的靶蛋白的整个周围表面显示的方法

    公开(公告)号：US08030064B2

    公开(公告)日：2011-10-04

    申请号：US10572224

    申请日：2003-12-30

    申请人： Sang Yup Lee ,  Tae Jung Park ,  Jong Pil Park ,  Seok Jae Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    发明人： Sang Yup Lee ,  Tae Jung Park ,  Jong Pil Park ,  Seok Jae Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    IPC分类号： C12N15/00 ,  C12N1/21

    CPC分类号： C40B40/02 ,  C12N15/1037

    摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

    摘要翻译： 本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。 更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离 特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。 根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平 现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

    MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME
    17.
    发明申请
    MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME 有权
    具有生产聚氨酯的高生产能力的突变微生物及使用其生产聚氨酯的方法

    公开(公告)号：US20100203599A1

    公开(公告)日：2010-08-12

    申请号：US12579052

    申请日：2009-10-14

    申请人： Sang Yup Lee ,  Zhi Gang Qian ,  Xiaoxia Xia ,  Yong Jae Jeon

    发明人： Sang Yup Lee ,  Zhi Gang Qian ,  Xiaoxia Xia ,  Yong Jae Jeon

    IPC分类号： C12P13/00 ,  C12N15/74 ,  C12N1/00 ,  C12N1/21

    CPC分类号： C12P13/001

    摘要： Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.

    摘要翻译： 提供具有产生高浓度腐胺的能力的突变微生物，其中涉及腐胺降解或利用途径的基因被灭活或缺失，其制备方法。 还提供了通过培养突变微生物以高产率产生腐胺的方法。 突变体微生物可用于生产高浓度腐胺，可广泛用于各种工业应用。

    Method For Extracellular Production of Target Proteins By Co-Expression of Ompf and Target Proteins
    20.
    发明申请
    Method For Extracellular Production of Target Proteins By Co-Expression of Ompf and Target Proteins 有权
    通过Ompf和靶蛋白的共表达的细胞外生产目标蛋白的方法

    公开(公告)号：US20080206814A1

    公开(公告)日：2008-08-28

    申请号：US11995689

    申请日：2005-07-15

    申请人： Sang Yup Lee ,  Jong Hyun Choi

    发明人： Sang Yup Lee ,  Jong Hyun Choi

    IPC分类号： C12P21/04 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K14/245 ,  C12N15/70

    摘要： The present invention relates to a method for secreting and producing a target protein into cell culture broth. More particularly, the invention relates to a microorganism co-transformed with a recombinant expression vector containing E. coli outer membrane protein F (OmpF) and a recombinant expression vector containing a target protein to be secreted into cell culture broth, as well as a method of secreting and producing the target protein into cell culture broth by culturing the microorganism. According to the invention, the target protein can be secreted into cell culture broth in a pure form without fusion with other proteins so that the efficient isolation and purification of the target protein is possible.

    摘要翻译： 本发明涉及一种在细胞培养液中分泌和产生靶蛋白的方法。 更具体地，本发明涉及与含有大肠杆菌外膜蛋白F（OmpF）的重组表达载体共同转化的微生物和含有要分泌到细胞培养液中的靶蛋白的重组表达载体，以及方法 通过培养微生物将靶蛋白分泌并产生到细胞培养液中。 根据本发明，靶蛋白可以以纯形式分泌到细胞培养肉汤中，而不与其它蛋白质融合，因此靶蛋白的有效分离和纯化是可能的。