公开(公告)号：US07022320B1

公开(公告)日：2006-04-04

申请号：US09501328

申请日：2000-02-09

Applicant: Michael D. Macklin ,  Deborah L. Fuller

Inventor： Michael D. Macklin ,  Deborah L. Fuller

IPC: A61K48/00 ,  A61K39/04 ,  C07H21/02 ,  C07H21/04

CPC classification number: A61K39/04 ,  A61K2039/51 ,  Y10S435/863

Abstract: Recombinant nucleic acid molecules are described. The molecules have a sequence or sequences encoding at least two M. tuberculosis antigens. Vectors and compositions containing these molecules are also described. In addition, compositions containing a cocktail of recombinant nucleic acid molecules having a sequence or sequences encoding one or more M. tuberculosis antigens are described. Methods of eliciting an immune response using these molecules and compositions are also described.

Abstract translation: 描述重组核酸分子。 分子具有编码至少两种结核分枝杆菌抗原的序列或序列。 还描述了含有这些分子的载体和组合物。 此外，描述了含有具有编码一种或多种结核分枝杆菌抗原的序列或序列的重组核酸分子的混合物的组合物。 还描述了使用这些分子和组合物引起免疫应答的方法。

公开(公告)号：US20050216959A1

公开(公告)日：2005-09-29

申请号：US11086657

申请日：2005-03-23

Applicant: Winston Brill ,  Dennis McCabe ,  Ning-Sun Yang

Inventor： Winston Brill ,  Dennis McCabe ,  Ning-Sun Yang

IPC: C12N15/09 ,  A01K67/00 ,  A61K9/14 ,  A61K38/45 ,  A61K47/02 ,  A61K48/00 ,  C12M1/00 ,  C12M3/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/87 ,  C12N15/89

CPC classification number: C12N15/87 ,  C12M35/02 ,  C12N15/895

Abstract: A method is disclosed for the convenient transformation of the somatic cells of animals. Somatic cell transformation is useful for medical and veterinary care of genetic diseases, and other therapeutic or animal improvement purposes. The method makes use of an electric discharge particle acceleration apparatus which can inject very small particles of gold or other dense material carrying genetic constructs coated on them into the living cells of animals. The animals not only live, but there is no visible bruising or bleeding at the site of the treatment. The method is particularly adaptable since the force of the particle injection in such a spark discharge apparatus is adjustable by adjustments to the voltage of the spark discharge.

Abstract translation: 公开了用于动物体细胞的方便转化的方法。 体细胞转化可用于遗传疾病的医学和兽医保健以及其他治疗或动物改善目的。 该方法利用放电粒子加速装置，其能够将非常小的金颗粒或其他致密材料携带在其上的动物细胞中。 动物不仅活着，而且在治疗部位没有可见的瘀伤或出血。 该方法特别适用，因为这种火花放电装置中的颗粒注入力可通过调节火花放电的电压来调节。

公开(公告)号：US5780100A

公开(公告)日：1998-07-14

申请号：US444173

申请日：1995-05-18

Applicant: Dennis E. McCabe ,  Richard J. Heinzen

Inventor： Dennis E. McCabe ,  Richard J. Heinzen

IPC: A61K48/00 ,  B05D7/22 ,  B05D3/04 ,  B05D3/12 ,  C12N15/00

CPC classification number: A61K48/00

Abstract: A method for depositing particles coated with biological substances onto the concave inner surface of a length of tubing includes the steps of preparing a uniform suspension of coated particles, introducing the particles into the tubing, placing the tubing into a generally horizontal position, removing the evaporable liquid after the particles have settled, and drying the particles. When dry, the tubing is cut into sample cartridges of appropriate length for use in a particle acceleration instrument. An apparatus for performing the method is also disclosed.

Abstract translation: 将生物物质涂覆的颗粒沉积到一段管道的凹内表面上的方法包括以下步骤：制备涂覆颗粒的均匀悬浮液，将颗粒引入管道中，将管道放置在大致水平的位置，去除可蒸发的 颗粒沉淀后的液体，并干燥颗粒。 当干燥时，将管道切成适当长度的样品盒，用于颗粒加速仪。 还公开了一种用于执行该方法的装置。

公开(公告)号：US08663657B2

公开(公告)日：2014-03-04

申请号：US10575087

申请日：2004-10-11

Applicant: James Fuller

Inventor： James Fuller

IPC: A61K31/70 ,  C12N15/11 ,  C12N15/00 ,  A61K39/00 ,  A61P43/00 ,  C12N15/87 ,  A61K9/50

CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C07K14/4716 ,  C07K14/4741 ,  C07K14/62 ,  C12N15/85 ,  C12N2710/16622 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/42 ,  C12N2830/60

Abstract: A nucleic acid construct comprising a chimeric promoter sequence and a cloning site for insertion of a coding sequence in operable linkage with the chimeric promoter, wherein the chimeric promoter sequence comprises: (a) a hCMV immediate early promoter sequence; (b) exon 1 and at least a part of exon 2 of the hCMV major immediate early gene; and (c) a heterologous intron provided in place of the intron A region of the hCMV major immediate early gene.

Abstract translation: 一种核酸构建体，其包含嵌合启动子序列和用于插入与嵌合启动子可操作连接的编码序列的克隆位点，其中所述嵌合启动子序列包含：（a）hCMV立即早期启动子序列; （b）外显子1和hCMV主要即时早期基因的外显子2的至少一部分; 提供代替hCMV主要立即早期基因的内含子A区域的异源内含子。

公开(公告)号：US20090017527A1

公开(公告)日：2009-01-15

申请号：US11752173

申请日：2007-05-22

Applicant: Joel R. HAYNES ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

Inventor： Joel R. HAYNES ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

IPC: C12M1/00

CPC classification number: A61K33/24 ,  A61K39/0011 ,  A61K39/015 ,  A61K39/12 ,  A61K39/145 ,  A61K39/21 ,  A61K39/292 ,  A61K45/06 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55572 ,  A61K2039/55577 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/895 ,  C12N2730/10122 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/16122 ,  C12N2760/16134 ,  Y02A50/412

Abstract: Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Abstract translation: 描述了可用于核酸免疫技术的试剂。 更具体地，描述了佐剂化的遗传疫苗组合物，以及使用这些组合物诱导针对所选抗原的增强的免疫应答的方法。

公开(公告)号：US20070009487A1

公开(公告)日：2007-01-11

申请号：US11482152

申请日：2006-07-07

Applicant: James Fuller

Inventor： James Fuller

IPC: A61K48/00 ,  A61K9/14

CPC classification number: A61K48/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/53 ,  C12N2730/10134

Abstract: Minimal promoter sequences are described. Reagents including a nucleic acid molecule which contains these minimal promoter sequences are also described. Methods for constructing these reagents, and methods for using these reagents are also described.

Abstract translation: 描述了最小的启动子序列。 还描述了包含含有这些最小启动子序列的核酸分子的试剂。 还描述了构建这些试剂的方法以及使用这些试剂的方法。

公开(公告)号：US20060228375A1

公开(公告)日：2006-10-12

申请号：US11453977

申请日：2006-06-16

Applicant: Dexiang Chen ,  Sangeeta Bhargava ,  Deborah Fuller

Inventor： Dexiang Chen ,  Sangeeta Bhargava ,  Deborah Fuller

IPC: A61K39/12 ,  A61K39/02 ,  A61K39/106 ,  A61K48/00

CPC classification number: A61K39/05 ,  A61K39/12 ,  A61K39/145 ,  A61K39/39 ,  A61K2039/5252 ,  A61K2039/541 ,  A61K2039/543 ,  A61K2039/55544 ,  A61K2039/55561 ,  A61K2039/57 ,  A61K2039/70 ,  C12N2760/16134

Abstract: Methods for generating an immune response at a mucosal surface are described. Compositions suitable for use in the methods for generating an immune response at a mucosal surface are also described. In addition, methods for treating or preventing a disease caused by the entry of a pathogen into the body of a subject via a mucosal surface are provided.

Abstract translation: 描述了在粘膜表面产生免疫应答的方法。 还描述了适用于在粘膜表面产生免疫应答的方法中的组合物。 此外，提供了用于治疗或预防由病原体经由粘膜表面进入受试者体内引起的疾病的方法。

公开(公告)号：US20050266021A1

公开(公告)日：2005-12-01

申请号：US11041875

申请日：2005-01-25

Applicant: Yuh-Fun Maa ,  Lu Zhao ,  Steven Prestrelski

Inventor： Yuh-Fun Maa ,  Lu Zhao ,  Steven Prestrelski

IPC: A61K9/16 ,  A61K39/12 ,  A61K39/02

CPC classification number: A61K9/1694 ,  A61K9/0021

Abstract: A gel-forming free-flowing powder suitable for use as a vaccine is prepared by spray-drying or spray freeze-drying an aqueous suspension that contains an antigen adsorbed to an aluminum salt or calcium salt adjuvant, a saccharide, an amino acid or a salt thereof, and a colloidal substance. Powder for vaccine purposes are also prepared by spray freeze-drying an aqueous suspension of such an adjuvant having an antigen adsorbed therein. Processes for forming these powder compositions are also described, as well as methods of using the compositions in a vaccination procedure.

Abstract translation: 适合用作疫苗的凝胶形成自由流动粉末通过喷雾干燥或喷雾冷冻干燥含有吸附在铝盐或钙盐佐剂，糖类，氨基酸或 其盐和胶态物质。 用于疫苗目的的粉末也可通过喷雾冷冻干燥其中吸附有抗原的佐剂的水悬浮液来制备。 还描述了用于形成这些粉末组合物的方法，以及在疫苗接种程序中使用该组合物的方法。

公开(公告)号：US20050148529A1

公开(公告)日：2005-07-07

申请号：US10751103

申请日：2004-01-05

Applicant: Connie Schmaljohn ,  James Fuller

Inventor： Connie Schmaljohn ,  James Fuller

IPC: A61K39/07 ,  A61K48/00

CPC classification number: A61K39/07 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/60

Abstract: Recombinant nucleic acid molecules are described. The molecules have a sequence or sequences encoding an antigen from Bacillus anthracis. Vectors and compositions containing these molecules are also described. Methods for eliciting an immune response using these molecules and compositions are also described.

Abstract translation: 描述重组核酸分子。 分子具有编码来自炭疽芽孢杆菌的抗原的序列或序列。 还描述了含有这些分子的载体和组合物。 还描述了使用这些分子和组合物引起免疫应答的方法。

公开(公告)号：US20040213798A1

公开(公告)日：2004-10-28

申请号：US10852740

申请日：2004-05-25

Applicant: PowderJect Vaccines, Inc.

Inventor： Yuh-Fun Maa ,  Lu Zhao ,  Steven J. Prestrelski

IPC: A61K039/00

CPC classification number: A61K9/1694 ,  A61K9/0021

Abstract: A gel-forming free-flowing powder suitable for use as a vaccine is prepared by spray-drying an aqueous suspension that contains an antigen adsorbed to an aluminum salt adjuvant, a saccharide, an amino acid or a salt thereof, and a colloidal substance. Processes for forming these powder compositions are also described, as well as methods of using the compositions in a vaccination procedure.

Abstract translation: 通过喷雾干燥含有吸附在铝盐佐剂，糖类，氨基酸或其盐上的抗原和胶态物质的水性悬浮液来制备适合用作疫苗的凝胶形成自由流动粉末。 还描述了用于形成这些粉末组合物的方法，以及在疫苗接种程序中使用该组合物的方法。