中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

134 records (took 0.023 seconds)

    RNA SEQUENCING METHODS
    12.
    发明公开
    RNA SEQUENCING METHODS 审中-公开

    公开(公告)号：US20230313289A1

    公开(公告)日：2023-10-05

    申请号：US18076214

    申请日：2022-12-06

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

    IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12N15/10

    CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/178 ,  C12Y207/07049

    Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

    RNA sequencing methods
    14.
    发明授权
    RNA sequencing methods 有权

    公开(公告)号：US11578363B2

    公开(公告)日：2023-02-14

    申请号：US16925778

    申请日：2020-07-10

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian Oberstrass ,  Gilad Almogy

    IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/6874

    Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

    SEQUENCING USING NON-NATURAL NUCLEOTIDES
    15.
    发明申请
    SEQUENCING USING NON-NATURAL NUCLEOTIDES 有权

    公开(公告)号：US20230022124A1

    公开(公告)日：2023-01-26

    申请号：US17713934

    申请日：2022-04-05

    Applicant: Ultima Genomics, Inc.

    Inventor： Gilad ALMOGY ,  Linda LEE

    IPC: C12Q1/6869 ,  C12Q1/6806

    Abstract: The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

    Methods and systems for sequence calling
    18.
    发明授权
    Methods and systems for sequence calling 有权

    公开(公告)号：US11276480B2

    公开(公告)日：2022-03-15

    申请号：US17406464

    申请日：2021-08-19

    Applicant: ULTIMA GENOMICS, INC.

    Inventor： Mark Pratt ,  Gilad Almogy ,  Avishai Bartov

    IPC: G16B40/10 ,  G16B30/10 ,  G16B45/00 ,  C12Q1/6806 ,  C12Q1/6869

    Abstract: The present disclosure provides methods and systems for accurate and efficient context-aware base calling of sequences. In an aspect, disclosed herein is a method for sequencing a nucleic acid molecule, comprising: (a) sequencing the nucleic acid molecule to generate a plurality of sequence signals; and (b) determining base calls of the nucleic acid molecule based at least in part on (i) the plurality of sequence signals and (ii) quantified context dependency for at least a portion of the plurality of sequence signals.

    RNA SEQUENCING METHODS
    19.
    发明申请
    RNA SEQUENCING METHODS 有权

    公开(公告)号：US20210147931A1

    公开(公告)日：2021-05-20

    申请号：US17158960

    申请日：2021-01-26

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

    IPC: C12Q1/6874 ,  C12N15/10

    Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

Patent Agency Ranking