公开(公告)号：US20040137556A1

公开(公告)日：2004-07-15

申请号：US10470673

申请日：2004-02-05

发明人： Roberto Spagnoli ,  Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse ,  Bruno Dumas ,  Denis Pompon ,  Jacques Winter

IPC分类号： C12P033/10 ,  C12N001/18 ,  C12N015/74

CPC分类号： C12Y114/15004 ,  C12P33/005 ,  C12P33/08 ,  C12P33/18 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y114/9901 ,  C12Y118/01002

摘要： The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.

摘要翻译： 本发明涉及从简单的碳源自主生产类固醇的转基因酵母菌株。 本发明还涉及从这样的酵母菌株生产类固醇的方法。

公开(公告)号：US20040067579A1

公开(公告)日：2004-04-08

申请号：US10462128

申请日：2003-06-16

申请人： Aventis Pharma S.A.

发明人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

IPC分类号： C12N015/00

CPC分类号： C12Y106/02004 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0095 ,  C12P33/00 ,  C12Y101/01053 ,  C12Y114/15004 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y118/01002

摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17null-hydroxy-progesterone; the conversion of 17null-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.

摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 该步骤选自：胆固醇转化为孕烯醇酮; 孕烯醇酮转化为孕酮; 孕酮转化为17α-羟基孕酮; 将17α-羟孕酮转化为皮质醇; 将皮质醇转化为氢化可的松，以及相应的控制顺序在所述宿主中有效。

公开(公告)号：US06171836B2

公开(公告)日：2001-01-09

申请号：US09099011

申请日：1998-06-17

申请人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

发明人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

IPC分类号： C12N902

CPC分类号： C12Y106/02004 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0095 ,  C12P33/00 ,  C12Y101/01053 ,  C12Y114/15004 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y118/01002

摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.

摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 该步骤选自：将胆固醇转化为孕烯醇酮;将孕烯醇酮转化为孕酮;将孕酮转化为17α-羟孕酮;将17α-羟孕酮转化为皮质醇;将皮质醇转化为氢化可的松， 并且相应的控制序列在所述主机中有效。

公开(公告)号：US5869283A

公开(公告)日：1999-02-09

申请号：US418085

申请日：1995-04-06

申请人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

发明人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N15/12 ,  C12P33/00 ,  C12N15/55 ,  C12N15/61

CPC分类号： C12Y106/02004 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0095 ,  C12P33/00 ,  C12Y101/01053 ,  C12Y114/15004 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y118/01002

摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:the conversion of cholesterol to pregnenolone;the conversion of pregnenolone to progesterone;the conversion of progesterone to 17.alpha.-hydroxy-progesterone;the conversion of 17.alpha.-hydroxyprogesterone to cortexolone;the conversion of cortexolone to hydrocortisone, and thecorresponding control sequences effective in said host.

摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 该步骤选自：胆固醇转化为孕烯醇酮; 孕烯醇酮转化为孕酮; 孕酮转化为17α-羟基孕酮; 将17α-羟基孕酮转化为皮质醇; 将皮质醇转化为氢化可的松，以及相应的控制顺序在所述宿主中有效。

公开(公告)号：US20230263829A1

公开(公告)日：2023-08-24

申请号：US18115846

申请日：2023-03-01

申请人： University of South Alabama Foundation for Research and Commercialization

发明人： Richard Eric Honkanen ,  Brandon Marshall D'Arcy ,  Mark Raymond Swingle

IPC分类号： A61K35/17 ,  C12N15/52 ,  C12N15/62 ,  C12N5/0783

CPC分类号： A61K35/17 ,  C12N15/52 ,  C12N15/62 ,  C12N5/0636 ,  C12Y103/01021 ,  C12N2820/60 ,  C12Y101/01051 ,  C12Y106/02004 ,  C12Y118/01002 ,  C12N2710/16141 ,  C12N2820/005 ,  C12Y114/15 ,  C07K2319/43 ,  C12Y103/99004 ,  C12Y101/01145 ,  C12Y114/15006 ,  C12N9/001

摘要： Compositions, methods, and systems for modifying sterol metabolism in a subject is disclosed. In some embodiments, the subjects may be administered one or more mammalian cells modified to express at least one sterol degrading enzyme derived from a bacterium. In many embodiments, the cell is a macrophage or monocyte stably expressing three or more enzymes that aid in opening the β ring of cholesterol. The disclosed compositions and methods may be useful in lowering cholesterol levels in a subject in need thereof. In some embodiments, the subject may have a genetic predisposition to atherosclerosis.

公开(公告)号：US20230151393A1

公开(公告)日：2023-05-18

申请号：US16766225

申请日：2019-01-16

申请人： FIRMENICH SA

发明人： Michel Schalk ,  Letizia Rocci ,  Fabienne Deguerry

IPC分类号： C12P7/02 ,  C12N5/10 ,  C12N15/63 ,  C12N15/52 ,  C12N9/02 ,  C12P5/00

CPC分类号： C12P7/02 ,  C12N5/10 ,  C12N15/63 ,  C12N15/52 ,  C12N9/0077 ,  C12Y114/15006 ,  C12P5/007

摘要： Described herein are the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. Also described herein are methods of oxidizing terpene molecules including contacting the cytochrome P450 with the terpene molecule intended to be oxidized. In particular, the method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. Also described herein is an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the disclosure.

公开(公告)号：US09534221B2

公开(公告)日：2017-01-03

申请号：US14160747

申请日：2014-01-22

申请人： National Jewish Health

发明人： Erwin W. Gelfand ,  Meiqin Wang ,  Yi Jia

IPC分类号： A61K31/451 ,  C12N15/113 ,  A61K31/444 ,  A61K31/58 ,  A61K31/7105

CPC分类号： C12N15/1137 ,  A61K31/444 ,  A61K31/451 ,  A61K31/58 ,  A61K31/7105 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12Y114/15006 ,  Y10S514/826

摘要： The present invention relates to methods and compositions for treating and/or preventing allergic diseases or conditions by inhibiting one or more components of the steroidogenic pathway.

摘要翻译： 本发明涉及通过抑制类固醇生成途径的一种或多种成分来治疗和/或预防过敏性疾病或病症的方法和组合物。

公开(公告)号：US07977065B2

公开(公告)日：2011-07-12

申请号：US12683642

申请日：2010-01-07

申请人： Roberto Spagnoli ,  Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse ,  Bruno Dumas ,  Denis Pompon ,  Jacques Winter

发明人： Roberto Spagnoli ,  Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse ,  Bruno Dumas ,  Denis Pompon ,  Jacques Winter

IPC分类号： C12P33/00 ,  C12P33/18 ,  C12P33/06 ,  C12N15/00 ,  C12N1/15 ,  C12P21/00

CPC分类号： C12Y114/15004 ,  C12P33/005 ,  C12P33/08 ,  C12P33/18 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y114/9901 ,  C12Y118/01002

摘要： The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.

摘要翻译： 本发明涉及从简单的碳源自主生产类固醇的转基因酵母菌株。 本发明还涉及从这样的酵母菌株生产类固醇的方法。

公开(公告)号：US07670829B2

公开(公告)日：2010-03-02

申请号：US10470673

申请日：2002-01-29

申请人： Roberto Spagnoli ,  Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse ,  Bruno Dumas ,  Denis Pompon ,  Jacques Winter

发明人： Roberto Spagnoli ,  Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse ,  Bruno Dumas ,  Denis Pompon ,  Jacques Winter

IPC分类号： C12N1/19 ,  C12N1/18 ,  C12P21/00 ,  C12P33/00 ,  C12P33/12 ,  C12P33/18 ,  C12P33/08 ,  C12N15/00 ,  C07H21/00

CPC分类号： C12Y114/15004 ,  C12P33/005 ,  C12P33/08 ,  C12P33/18 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y114/9901 ,  C12Y118/01002

摘要： The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.

摘要翻译： 本发明涉及从简单的碳源自主生产类固醇的转基因酵母菌株。 本发明还涉及从这样的酵母菌株生产类固醇的方法。

公开(公告)号：US5547868A

公开(公告)日：1996-08-20

申请号：US75193

申请日：1993-06-09

申请人： Walter L. Miller ,  Jennifer A. Harikrishna ,  Stephen M. Black

发明人： Walter L. Miller ,  Jennifer A. Harikrishna ,  Stephen M. Black

IPC分类号： C12N1/15 ,  C12N1/21 ,  C12N9/02 ,  C12P33/00 ,  C07K14/80

CPC分类号： C12N9/0081 ,  C12N9/0095 ,  C12P33/005 ,  C12Y114/15006 ,  C12Y118/01002 ,  C07K2319/00

摘要： Fusion enzymes having multiple segments of different biological activity including one segment having P450scc activity and at least one segment having electron-transfer activity for transferring electrons to P450scc are described along with genetic constructs for production of such enzymes and methods for their use. Methods of their use include cholesterol degradation in vitro or in vivo as well as conversion of cholesterol to other useful steroidal products including pregnenolone.

摘要翻译： 与具有P450scc活性的一个区段和具有电子转移活性的至少一个区段具有不同生物学活性的多个区段的融合酶与用于产生这些酶的遗传构建体及其使用方法一起被描述。 其使用方法包括体外或体内的胆固醇降解以及胆固醇转化为其他有用的甾体产物，包括孕烯醇酮。