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    Modified yeasts and uses thereof, in particular for producing steroid derivatives
    3.
    发明授权
    Modified yeasts and uses thereof, in particular for producing steroid derivatives 有权
    改良的酵母及其用途，特别是用于生产类固醇衍生物

    公开(公告)号：US08173402B2

    公开(公告)日：2012-05-08

    申请号：US13006607

    申请日：2011-01-14

    申请人： Bruno Dumas ,  Gilles Cauet ,  Eric Degryse ,  Tilman Achstetter

    发明人： Bruno Dumas ,  Gilles Cauet ,  Eric Degryse ,  Tilman Achstetter

    IPC分类号： C12P7/00 ,  C12N15/00 ,  C12N1/20 ,  C12N1/14 ,  C12N9/00 ,  C12N9/04 ,  C12Q1/34 ,  C12Q1/26 ,  C07H21/04 ,  C07H21/02

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或提高方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾类衍生物的产品。

    Modified yeasts and uses thereof, in particular for producing steroid derivatives
    4.
    发明授权
    Modified yeasts and uses thereof, in particular for producing steroid derivatives 有权
    改良的酵母及其用途，特别是用于生产类固醇衍生物

    公开(公告)号：US07879592B2

    公开(公告)日：2011-02-01

    申请号：US10343993

    申请日：2001-07-24

    申请人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    发明人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    IPC分类号： C12N1/14 ,  C12N1/20 ,  C12N9/04 ,  C12N15/00 ,  C12Q1/68 ,  C07H21/04 ,  C07H21/02 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/26 ,  C12N9/00

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或增加方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾族衍生物的产品。

    MODIFIED YEASTS AND USES THEREOF, IN PARTICULAR FOR PRODUCING STEROID DERIVATIVES
    5.
    发明申请
    MODIFIED YEASTS AND USES THEREOF, IN PARTICULAR FOR PRODUCING STEROID DERIVATIVES 有权
    改良的YEASTS及其用途，特别是生产甾类衍生物

    公开(公告)号：US20110104749A1

    公开(公告)日：2011-05-05

    申请号：US13006607

    申请日：2011-01-14

    申请人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    发明人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    IPC分类号： C12P33/00

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或提高方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾类衍生物的产品。

    Method for preparing steroids modified by yeast fermentation
    8.
    发明授权
    Method for preparing steroids modified by yeast fermentation 失效
    通过酵母发酵制备类固醇的方法

    公开(公告)号：US07033779B1

    公开(公告)日：2006-04-25

    申请号：US10089803

    申请日：2000-10-04

    申请人： Gilles Cauet ,  Eric Degryse ,  Pedro Vico ,  Richard Lathe

    发明人： Gilles Cauet ,  Eric Degryse ,  Pedro Vico ,  Richard Lathe

    IPC分类号： C12N1/18 ,  C12N9/10 ,  C12N15/00 ,  C12P7/02 ,  C07H21/04

    CPC分类号： C12N9/0077 ,  C12P33/06

    摘要： The invention concerns a novel method for producing hydroxylated and/or acetylated steroid comprising steps which consist in: culturing yeasts on a medium comprising at least a precursor of such hydroxylated and/or acetylated steroids; then isolating the hydroxylated and/or acetylated steroids from the medium after bioconversion. Said method is characterized in that said yeasts are yeasts transformed so as to express the product of Cyp7b gene. The invention also concerns the modified yeast strains.

    摘要翻译： 本发明涉及用于生产羟基化和/或乙酰化类固醇的新方法，其包括以下步骤：在包含至少一种羟基化和/或乙酰化类固醇前体的培养基上培养酵母; 然后在生物转化后从培养基中分离羟基化和/或乙酰化类固醇。 所述方法的特征在于所述酵母是转化酵母以表达Cyp7b基因的产物。 本发明还涉及修饰的酵母菌株。

    Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
    9.
    发明授权
    Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene 失效
    突变的5-烯醇丙酮酸莽草酸-3-磷酸合酶，编码所述蛋白质的基因和含有所述基因的转化植物

    公开(公告)号：US06566587B1

    公开(公告)日：2003-05-20

    申请号：US08945144

    申请日：1998-01-20

    申请人： Michel Lebrun ,  Alain Sailland ,  Georges Freyssinet ,  Eric Degryse

    发明人： Michel Lebrun ,  Alain Sailland ,  Georges Freyssinet ,  Eric Degryse

    IPC分类号： C12N1582

    CPC分类号： C12N9/1092 ,  C12N15/8275

    摘要： The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.

    摘要翻译： 本发明涉及编码经修饰的5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）酶的植物来源的DNA分子，其中通常将编码成熟EPSPS序列的苏氨酸残基的第一EPSPS编码序列修饰为编码 修饰成熟的EPSPS序列，并且通常编码成熟EPSPS序列的脯氨酸残基的第二EPSPS编码序列被修饰以编码成熟EPSPS序列的丝氨酸，其中第一和第二残基分别位于成熟的EPSPS序列的相对位置102和106 由所述DNA分子编码的EPSPS序列; 以及产生对膦酰基甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的转基因植物。 基本上保持野生型酶的催化活性的突变酶允许植物对膦酰基甲基甘氨酸家族的除草剂的耐受性或耐受性增加，并允许植物，其器官的基本正常生长或发育， 组织或细胞。