公开(公告)号：US20040248303A1

公开(公告)日：2004-12-09

申请号：US10760752

申请日：2004-01-20

申请人： Syngenta Participations AG

发明人： Rebecca M. Cade ,  Robert A. Dietrich

IPC分类号： C12N015/82 ,  C07H021/04 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10

CPC分类号： C12N15/8281 ,  C07K14/415 ,  C12N15/8282

摘要： The Arabidopsis NI16 gene was isolated in a yeast 2-hybrid screen via its interaction with the NIM1 protein and encodes a protein involved in the regulation of SAR gene expression in plants. NI16 is strongly induced in NIM1-overexpressing plants treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH). The nucleic acid sequence of the Arabidopsis NI16 promoter is disclosed herein.

摘要翻译： 通过与NIM1蛋白的相互作用，在酵母2-杂交筛选中分离拟南芥NI16基因，并编码参与植物中SAR基因表达调控的蛋白质。 NI16在用苯并（1,2,3）噻二唑-7-硫代硫酸S-甲酯（BTH）处理的NIM1过表达植物中强烈诱导。 本文公开了拟南芥NI16启动子的核酸序列。

公开(公告)号：US20040224413A1

公开(公告)日：2004-11-11

申请号：US10855854

申请日：2004-05-27

发明人： Edgar B. Cahoon ,  William D. Hitz ,  Anthony J. Kinney ,  Steven J. Vollmer

IPC分类号： C12N015/82 ,  C12Q001/68 ,  C07H021/04 ,  A01H001/00 ,  C12N015/87 ,  A01H005/00 ,  C07K014/00 ,  C12N005/04 ,  C12N005/10 ,  A01H005/10

CPC分类号： C07H21/04 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/8247

摘要： This invention relates to an isolated nucleic acid fragment encoding an enzyme involved in lipid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme involved in lipid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme involved in lipid biosynthesis in a transformed host cell.

摘要翻译： 本发明涉及编码参与脂质生物合成的酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码参与脂质生物合成的全部或部分酶的嵌合基因的构建，其中嵌合基因的表达导致生产参与脂质生物合成的酶水平的变化 转化的宿主细胞。

公开(公告)号：US20040088758A1

公开(公告)日：2004-05-06

申请号：US10607803

申请日：2003-06-26

申请人： Consejo Superior de Investigaciones Cientificas (CSIC)

发明人： Enrique Martinez Force ,  Juan Munoz-Ruz ,  Jose M. Fernandez Martinez ,  Rafael Garces

IPC分类号： C12N015/82 ,  C12N015/87 ,  A01H005/00 ,  C12N005/10 ,  C12N005/02

CPC分类号： A23D7/003 ,  A01H5/10 ,  A21D2/165 ,  A23D7/001 ,  A23D9/00 ,  A23G3/346 ,  A23G9/327 ,  A23G9/52 ,  A23G2200/08 ,  A23K10/30 ,  A23L23/00 ,  A61K8/922 ,  A61K8/97 ,  A61Q1/06 ,  A61Q5/00 ,  A61Q19/00

摘要： Meal from sunflower plants that produce seeds with an extractable oil having an oleic acid content of more than 40% by weight and a stearic acid content of more than 12% by weight is disclosed.

摘要翻译： 公开了具有油酸含量大于40重量％且硬脂酸含量大于12重量％的可萃取油产生种子的向日葵植物的膳食。

公开(公告)号：US20040055032A1

公开(公告)日：2004-03-18

申请号：US10169045

申请日：2002-11-18

发明人： Stefan Henkes ,  Ryouing Chen ,  Nocha van Thielen ,  Oswaldo da Costa e Silva

IPC分类号： C12N015/82 ,  G01N033/53 ,  C12N015/87 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C07K014/00 ,  C12N005/10

CPC分类号： C07K14/415 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/14 ,  C12N15/8273 ,  C12N15/8282

摘要： A transgenic plant transformed by a pyrophosphatase stress-related protein (PPSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PPSRP, and isolated nucleic acid coding PPSRP, and vectors and host cells containing the latter. Further provided are methods of producing transgenic plants expressing PPSRP, methods of identifying novel PPSRP, and methods of modifying the expression of PPSRP in plants.

摘要翻译： 通过焦磷酸酶应激相关蛋白（PPSRP）编码核酸转化的转基因植物，其中核酸序列在植物中的表达与植物的野生型品种相比，导致对环境胁迫的耐受性增加。 还提供了由转基因植物生产的农产品，包括种子。 还提供了分离的PPSRP和分离的编码PPSRP的核酸，以及含有后者的载体和宿主细胞。 还提供了生产表达PPSRP的转基因植物的方法，鉴定新型PPSRP的方法以及修饰植物中PPSRP表达的方法。

公开(公告)号：US20030188346A1

公开(公告)日：2003-10-02

申请号：US09800130

申请日：2001-03-06

发明人： Scott R. Baerson ,  Damian J. Rodriguez ,  Gregory R. Heck

IPC分类号： C12N015/82 ,  C12N005/04 ,  C12N005/10 ,  C12N015/29 ,  A01H005/00

CPC分类号： C12N9/1092 ,  C12N15/8275

摘要： The methods and materials disclosed herein are directed to glyphosate herbicide tolerance in plants. In particular, the isolation of a glyphosate resistant EPSP synthase coding sequence and its regulatory elements from Eleusine indica. The coding sequence and regulatory sequences are useful to genetically engineer plants for tolerance to glyphosate herbicide.

公开(公告)号：US20030073829A1

公开(公告)日：2003-04-17

申请号：US09116676

申请日：1998-07-16

发明人： NIKA ADHAM ,  BETH BOROWSKY ,  NIGEL LEVENS ,  RADEK C. SKODA

IPC分类号： A01N037/18 ,  A61K038/00 ,  C07H021/04 ,  A61K031/70 ,  A01N043/04 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  C07K005/00 ,  C07K007/00 ,  C07K016/00 ,  C07K017/00 ,  C12N005/00 ,  C12N005/02 ,  C12N005/06 ,  C12N005/10 ,  C12N015/85 ,  C12N015/87

CPC分类号： A61K31/70 ,  A61K38/00 ,  C07K14/705 ,  C12N2799/026

摘要： This invention provides an isolated nucleic acid encoding a polypeptide, a purified polypeptide, vectors comprising isolated nucleic acid encoding a polypeptide, cells comprising such vectors, antibodies directed to a polypeptide, nucleic acid probes useful for detecting nucleic acid encoding a polypeptide, antisense oligonucleotides complementary to unique sequences of nucleic acid encoding a polypeptide, nonhuman transgenic animals which express DNA encoding a normal or a mutant polypeptide, methods of isolating a polypeptide, methods of treatment eating disorders as well as methods of determining binding of compounds to polypeptides.

摘要翻译： 本发明提供了编码多肽的分离的核酸，纯化的多肽，包含分离的编码多肽的核酸，包含此类载体的细胞，针对多肽的抗体，可用于检测编码多肽的核酸的核酸探针，反义寡核苷酸互补 编码多肽的独特的核酸序列，表达编码正常或突变多肽的DNA的非人类转基因动物，分离多肽的方法，进食障碍的治疗方法以及确定化合物与多肽结合的方法。

公开(公告)号：US20030050463A1

公开(公告)日：2003-03-13

申请号：US10134493

申请日：2002-04-30

发明人： Gregory P. Pogue ,  John A. Lindbo ,  Michael J. McCulloch ,  Jonathan E. Lawrence ,  Cynthia S. Gross ,  Stephen J. Garger

IPC分类号： A01H005/00 ,  C07H021/04 ,  C12N005/04 ,  C12P021/02 ,  C07K005/00 ,  A61K038/04 ,  C12N015/82 ,  C12P021/06 ,  C12N015/87 ,  C12N015/09 ,  C12N015/70 ,  C07K007/00 ,  C07K017/00 ,  C12N005/10

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K14/445 ,  C07K2319/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2770/00022

摘要： The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

公开(公告)号：US20020142456A1

公开(公告)日：2002-10-03

申请号：US09789306

申请日：2001-02-20

申请人： Amgen Inc.

发明人： Natasha Hernday

IPC分类号： C12P021/08 ,  C12N005/10 ,  C12N005/06 ,  C12N005/02 ,  C12N005/00 ,  A61K038/00 ,  C07K017/00 ,  C07K016/00 ,  C07K007/00 ,  C07K005/00 ,  C12N015/74 ,  C12N015/70 ,  C12N015/63 ,  C12N015/09 ,  C12N015/00 ,  C12N001/00 ,  C12P021/06 ,  C07H021/04

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K38/00

摘要： This invention provides for canine OB protein and associated nucleic acids, vectors, host cells, processes for production, related compositions, and methods for making and using thereof.

摘要翻译： 本发明提供了犬OB蛋白和相关核酸，载体，宿主细胞，生产方法，相关组合物及其制备和使用方法。

公开(公告)号：US20020132306A1

公开(公告)日：2002-09-19

申请号：US09819098

申请日：2001-04-11

发明人： Randal J. Kaufman ,  Steven W. Pipe ,  Kagehiro Amano

IPC分类号： A01N037/18 ,  A61K038/00 ,  C07H021/04 ,  C12P021/04 ,  A61K035/14 ,  C07K017/00 ,  C07K016/00 ,  C07K014/00 ,  C07K001/00 ,  C12N005/10 ,  C12N005/06 ,  C12N005/02 ,  C12N005/00 ,  C12N015/74 ,  C12N015/70 ,  C12N015/63 ,  C12N015/09 ,  C12N015/00

CPC分类号： C07K14/755 ,  A61K38/00

摘要： The present invention provides novel purified and isolated nucleic acid sequences encoding procoagulant-active FVIII proteins. The nucleic acid sequences of the present invention encode amino acid sequences corresponding to known human FVIII sequences, wherein residue Phe3O9 is mutated. The nucleic acid sequences of the present invention also encode amino acid sequences corresponding to known human FVIII sequences, wherein the APC cleavage sites, Arg336 and Ile562, are mutated. The nucleic acid sequences of the present invention further encode amino acid sequences corresponding to known human FVIII sequences, wherein the B-domain is deleted, the von Willebrand factor binding site is deleted, a thrombin cleavage site is mutated and an amino acid sequence spacer is inserted between the A2- and A3-domains. Methods of producing the FVIII proteins of the invention, nucleotide sequences encoding such proteins, pharmaceutical compositions containing the nucleotide sequences or proteins, as well as methods of treating patients suffering from hemophilia, are also provided.

摘要翻译： 本发明提供了编码促凝血活性FVIII蛋白的新型纯化和分离的核酸序列。 本发明的核酸序列编码对应于已知人FVIII序列的氨基酸序列，其中残基Phe3O9被突变。 本发明的核酸序列还编码对应于已知人FVIII序列的氨基酸序列，其中APC切割位点Arg336和Ile562突变。 本发明的核酸序列进一步编码对应于已知人FVIII序列的氨基酸序列，其中缺失B结构域，缺失血管性血友病因子结合位点，突变凝血酶切割位点，氨基酸序列间隔物为 插入在A2-和A3-域之间。 还提供了产生本发明的FVIII蛋白的方法，编码这种蛋白质的核苷酸序列，含有核苷酸序列或蛋白质的药物组合物，以及治疗患有血友病的患者的方法。

公开(公告)号：US20020078475A1

公开(公告)日：2002-06-20

申请号：US09899645

申请日：2001-07-05

申请人： Pioneer Hi-Bred International, Inc.

发明人： Chun Ping Li ,  Peizhong Zheng ,  Scott E. Nichols

IPC分类号： C12N015/82 ,  C12N005/04 ,  C12N015/29 ,  C12N015/87 ,  A01H005/00 ,  A01H005/10 ,  C12N015/63 ,  C12N005/10 ,  C07K014/00 ,  C07K017/00 ,  C12N015/09 ,  C12P007/64

CPC分类号： C12N9/16

摘要： The invention relates to the genetic manipulation of plants to increase oil accumulation in plant tissues, particularly seeds. Methods for decreasing null-oxidation in plants and optimizing oil accumulation in a seeds are provided. The methods find use in increasing the accumulation of oil or particular oil constituents in plant seeds. Isolated nucleotide molecules, isolated proteins, expression cassettes and transformed plants, plant tissues and plant cells are additionally provided.

摘要翻译： 本发明涉及植物的遗传操作以增加植物组织中尤其是种子中的积聚。 提供了减少植物中β-氧化和优化种子中油积聚的方法。 这些方法用于增加植物种子中油或特定油成分的积累。 另外提供分离的核苷酸分子，分离的蛋白质，表达盒和转化植物，植物组织和植物细胞。