公开(公告)号：US11939613B2

公开(公告)日：2024-03-26

申请号：US17474708

申请日：2021-09-14

Applicant: BIOMEDICAN, INC.

Inventor： Maxim Mikheev ,  Difeng Gao

IPC: C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12P7/22 ,  C12P17/06

CPC classification number: C12P17/06 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01034 ,  C12Y121/03007 ,  C12Y121/03008 ,  C12Y203/0102 ,  C12Y203/01206 ,  C12Y404/01026 ,  C12Y604/01002

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

公开(公告)号：US20240093253A1

公开(公告)日：2024-03-21

申请号：US17767957

申请日：2020-10-12

Applicant: National University of Singapore

Inventor： Kevin Jie Han Lim ,  Maybelle Darlene Kho Go ,  Wen Shan Yew

IPC: C12P17/06 ,  C12N9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52 ,  C12N15/81

CPC classification number: C12P17/06 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/81 ,  C12Y203/01086 ,  C12Y203/01206 ,  C12Y205/01039 ,  C12Y404/01026 ,  C12Y602/01012 ,  C12Y602/01025 ,  C12Y602/0103 ,  C12N2800/102

Abstract: A recombinant cell of Saccharomyces cerevisiae that includes in its genome nucleic acids encoding cannabinoid biosynthetic pathway genes. A cannabinoid is produced by the recombinant cell in the presence of a cannabinoid precursor substrate and at least one of the cannabinoid biosynthetic pathway genes is from an organism other than Cannabis sativa, wherein the at least one of the cannabinoid biosynthetic pathway genes encodes a prenyltransferase. In an embodiment, the prenyltransferase is NphB from Streptomyces sp. having the amino acid sequence of any one of SEQ ID NOs: 8-11. Also disclosed is a method for producing a cannabinoid with the recombinant cell and the cannabinoid precursor substrate.

公开(公告)号：US11818994B2

公开(公告)日：2023-11-21

申请号：US17518882

申请日：2021-11-04

Applicant: Agriculture Victoria Services PTY LTD

Inventor： German Carlos Spangenberg ,  Kathryn Michaela Guthridge ,  Ross Mann ,  Timothy Ivor Sawbridge ,  Inoka Kumari Hettiarachchige ,  Piyumi Niroshini Ekanayake ,  Natasha Denise Brohier ,  Simone Jane Rochfort ,  Jacqueline Edwards

IPC: C12N15/82 ,  A01H17/00 ,  C12Q1/689 ,  C12Q1/6895 ,  C12P5/00 ,  C12N1/14 ,  C12N1/20 ,  C07D493/00 ,  C07D493/08 ,  A01N63/30 ,  C12N15/10 ,  C12P17/06 ,  C12R1/01 ,  C12R1/645

CPC classification number: A01H17/00 ,  A01N63/30 ,  C07D493/00 ,  C07D493/08 ,  C12N1/14 ,  C12N1/145 ,  C12N1/20 ,  C12N1/205 ,  C12N15/1003 ,  C12P5/007 ,  C12P17/06 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/156 ,  C12R2001/01 ,  C12R2001/645 ,  A01N63/30 ,  A01N63/30

Abstract: The present invention relates to endophytes, in particular endophytes associated with plants of the Brachiaria-Urochloa species complex, plants infected with the endophytes, products produced by the endophytes, and related methods.

公开(公告)号：US11746364B2

公开(公告)日：2023-09-05

申请号：US17191051

申请日：2021-03-03

Applicant: Whitehead Institute for Biomedical Research

Inventor： Tomás Pluskal ,  Jing-Ke Weng

IPC: C12P17/06 ,  C12P17/00 ,  C12P7/26

CPC classification number: C12P17/06 ,  C12P7/26 ,  C12Y301/27005

Abstract: Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.

公开(公告)号：US20230270148A1

公开(公告)日：2023-08-31

申请号：US18046698

申请日：2022-10-14

Applicant: Conagen Inc. ,  Sweegen, Inc.

Inventor： Shari Mahon ,  Rui Zhou ,  Steven Chen

IPC: A23L27/00 ,  A23L2/56 ,  A23L33/105 ,  A23F3/40 ,  A61K9/00 ,  A61K47/26 ,  C12P17/06

CPC classification number: A23L27/86 ,  A23L2/56 ,  A23L33/105 ,  A23F3/405 ,  A61K9/0095 ,  A61K47/26 ,  C12P17/06

Abstract: The present invention relates, at least in part, to compounds and compositions that can be used to mask, block, or reduce the bitter taste present in various orally consumable products. The present invention also relates to methods of using bitterness blocking compounds and compositions to mask the bitterness of various orally consumable products, hence making such orally consumable products more palatable. The present invention further relates to an orally consumable product with reduced bitterness.

公开(公告)号：US20230193221A1

公开(公告)日：2023-06-22

申请号：US18049221

申请日：2022-10-24

Applicant: The Regents of the University of California

Inventor： James U. Bowie ,  Meaghan Valliere ,  Tyler P. Korman ,  Nicholas Woodall

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/42 ,  C12P9/00 ,  C12P17/06

CPC classification number: C12N9/1085 ,  C12P7/22 ,  C12P7/42 ,  C12P9/00 ,  C12P17/06 ,  C12Y205/01039

Abstract: Provided is an enzyme useful for prenylation and recombinant pathways for the production of cannabinoids, cannabinoid precursors and other prenylated chemicals in a cell free system as well and recombinant microorganisms that catalyze the reactions.

公开(公告)号：US20230167473A1

公开(公告)日：2023-06-01

申请号：US17780423

申请日：2021-11-17

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Chuang XUE ,  Mingming QI ,  Wenqiang ZHANG

IPC: C12P17/06 ,  C12N15/52 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P17/06 ,  C12N15/52 ,  C12N9/0004 ,  C12Y121/99 ,  C12N9/0006 ,  C12Y101/01157 ,  C12N9/001 ,  C12Y103/01044 ,  C12N9/1029 ,  C12Y203/01009 ,  C12N9/88 ,  C12Y402/01017 ,  C12Y404/01026 ,  C12N9/93 ,  C12Y604/01002

Abstract: A recombinant host cell capable of biosynthesizing cannabichromenic acid and a construction method thereof, and a method for biosynthesizing cannabichromenic acid through the recombinant host cell. Saccharomyces cerevisiae is taken as a host. First, cannabigerolic acid synthase and cannabichromenic acid synthase are over-expressed in the host; then, a metabolic pathway of a precursor compound, olivetolic acid, synthesizing cannabichromenic acid from saccharides is constructed in the host, a metabolic pathway for hexanoic acid to olivetolic acid is further constructed in the host, an endogenous mevalonate pathway of the host and a metabolic pathway of acetyl-CoA are optimized, cannabichromenic acid synthase is rationally designed, highly active cannabichromenic acid synthase is screened out, and finally, a cannabichromene pathway is located to peroxisomes and lipid droplets by using the cell compartmentalization principle to obtain recombinant Saccharomyces cerevisiae capable of biosynthesizing cannabichromenic acid.

公开(公告)号：US20230102194A1

公开(公告)日：2023-03-30

申请号：US17801990

申请日：2021-02-26

Applicant: CAS Center for Excellence in Molecular Plant Sciences

Inventor： Yong Wang ,  Yuwei Sun ,  Zhuo Chen

IPC: C12N9/02 ,  C12P7/26 ,  C12P17/06 ,  C12N15/70 ,  C12P19/18

Abstract: Provided are novel flavone hydroxylases, a microorganism for synthesizing flavone C-glycoside compounds, and use thereof. The present inventor obtains novel flavones hydroxylates PhF2H and PhF3′H by cloning, which belong to cytochrome P450 hydroxylates and have the function of hydroxylating specific positions of compounds. Further, the present inventor, by modifying the enzymes and combining a C-glycoside glycosyltransferase and assembly of a synthesis pathway of a flavone precursor, efficiently synthesizes flavone C-glycoside compounds such as oriention, isooriention, vitexin and isovitexin, and related intermediates such as eriodictyol and 2-hydroxynaringenin in the synthesis pathway thereof in an artificial recombinant expression system.

公开(公告)号：US20230029027A1

公开(公告)日：2023-01-26

申请号：US17602676

申请日：2020-04-13

Applicant: RENEW BIOPHARMA, INC.

Inventor： Michael MENDEZ ,  Joseph NOEL ,  Michael BURKART ,  Jeremy LANOISELEE ,  Kyle BOTSCH ,  Matthew SAUNDERS

IPC: C12N9/10 ,  C12P17/06 ,  C12P7/42 ,  C12P7/22

Abstract: The disclosure relates to the biosynthesis of cannabinoids and related prenylated phenolic compounds using recombinant enzymes. In particular, the disclosure provides recombinant prenyltransferase enzymes engineered to produce a greater amount of a desired product, or to have a greater ability to catalyze a reaction using a desired substrate, as compared to the wild type prenyltransferase. The disclosure also provides methods of preparing such recombinant enzymes; as well as methods of use thereof in improving the biosynthesis of cannabinoids and related prenylated phenolic compounds.

公开(公告)号：US20220403429A1

公开(公告)日：2022-12-22

申请号：US17844835

申请日：2022-06-21

Applicant: The Trustees of Princeton University

Inventor： Jose L. Avalos ,  Makoto A. Lalwani ,  Mark P. Brynildsen

IPC: C12P17/06 ,  C12N1/20 ,  C12N1/16 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74

Abstract: Microbial consortia exert great influence over the physiology of humans, animals, plants, and ecosystems. However, difficulty in controlling their composition and population dynamics have limited their application in medicine, agriculture, biotechnology, and the environment. The approach disclosed herein provides an effective method to dynamically control population compositions in microbial consortia, which we demonstrate in the context of co-culture fermentations for chemical production. Co-culture fermentations can improve chemical production from complex biosynthetic pathways over monocultures by distributing enzymes across multiple strains, thereby reducing metabolic burden, overcoming endogenous regulatory mechanisms, or exploiting natural traits of different microbial species. However, stabilizing and optimizing microbial sub-populations for maximal chemical production remains a major obstacle in the field. An optogenetic circuit, called OptoTA, is disclosed for regulating a toxin-antitoxin system, which enables tunability of, e.g., Escherichia coli growth using only blue light. With the disclosed system, one can control population ratios of co-cultures of, e.g., E. coli and Saccharomyces cerevisiae containing different metabolic modules of biosynthetic pathways. Results reveal that intermediate light duty cycles improve chemical production by establishing optimal co-culture populations.