ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    22.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 有权
    用于植物基因工程的土地垫

    公开(公告)号:US20110191899A1

    公开(公告)日:2011-08-04

    申请号:US13011735

    申请日:2011-01-21

    CPC classification number: C12N15/8201 C12N15/8213 C12N15/8241

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    Abstract translation: 一种用于生产转基因植物的方法包括提供核酸分子,其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点,其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    Method and apparatus for flexible fluid delivery for cooling desired hot spots in a heat producing device
    24.
    发明授权
    Method and apparatus for flexible fluid delivery for cooling desired hot spots in a heat producing device 有权
    用于冷却发热装置中所需热点的柔性流体输送的方法和装置

    公开(公告)号:US06988534B2

    公开(公告)日:2006-01-24

    申请号:US10439635

    申请日:2003-05-16

    Abstract: A heat exchanger apparatus and method of manufacturing comprising: an interface layer for cooling a heat source and configured to pass fluid therethrough, the interface layer having an appropriate thermal conductivity and a manifold layer for providing fluid to the interface layer, wherein the manifold layer is configured to achieve temperature uniformity in the heat source preferably by cooling interface hot spot regions. A plurality of fluid ports are configured to the heat exchanger such as an inlet port and outlet port, whereby the fluid ports are configured vertically and horizontally. The manifold layer circulates fluid to a predetermined interface hot spot region in the interface layer, wherein the interface hot spot region is associated with the hot spot. The heat exchanger preferably includes an intermediate layer positioned between the interface and manifold layers and optimally channels fluid to the interface hot spot region.

    Abstract translation: 一种热交换器装置和制造方法,包括:用于冷却热源并被配置为使流体通过其中的界面层,所述界面层具有适当的导热性和用于向界面层提供流体的歧管层,其中所述歧管层为 被配置为优选地通过冷却界面热点区域来实现热源中的温度均匀性。 多个流体端口被配置到诸如入口和出口的热交换器,由此流体端口被垂直和水平地配置。 歧管层将流体循环到界面层中的预定界面热点区域,其中界面热点区域与热点相关联。 热交换器优选地包括位于界面和歧管层之间的中间层,并且将流体最佳地通向界面热点区域。

    Recovery of isoflavones from aqueous mixtures using zeolites or molecular sieves
    25.
    发明申请
    Recovery of isoflavones from aqueous mixtures using zeolites or molecular sieves 审中-公开
    使用沸石或分子筛从含水混合物中回收异黄酮

    公开(公告)号:US20050202139A1

    公开(公告)日:2005-09-15

    申请号:US10983123

    申请日:2004-11-05

    CPC classification number: A23L3/3508 A23L3/3571 A23L3/358 C07D311/36

    Abstract: The present invention provides a process using a zeolite or molecular sieve for recovering isoflavones from aqueous mixtures, such as soy whey and other biological waste products. A zeolite, such as a large pore, hydrophobic zeolite, has a significantly higher affinity for isoflavones than conventional polymeric adsorbents, and has essentially no affinity for the undesired oligosaccharides raffinose and stachyose.

    Abstract translation: 本发明提供使用沸石或分子筛从水性混合物如大豆乳清和其它生物废物中回收异黄酮的方法。 诸如大孔疏水沸石之类的沸石对异黄酮具有比常规聚合物吸附剂显着更高的亲和性,并且对不想要的寡糖棉子糖和水苏糖基本上没有亲和力。

    Engineered landing pads for gene targeting in plants
    29.
    发明授权
    Engineered landing pads for gene targeting in plants 有权
    用于植物中基因靶向的设计着陆垫

    公开(公告)号:US08802921B2

    公开(公告)日:2014-08-12

    申请号:US13011735

    申请日:2011-01-21

    CPC classification number: C12N15/8201 C12N15/8213 C12N15/8241

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    Abstract translation: 一种用于生产转基因植物的方法包括提供核酸分子,其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点,其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

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