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    Polygenic trait determinants: maize dwarf mosaic virus
    1.
    依法登记的发明
    Polygenic trait determinants: maize dwarf mosaic virus 失效
    多基因性状决定因素：玉米矮花叶病毒

    公开(公告)号：USH1498H

    公开(公告)日：1995-11-07

    申请号：US50965

    申请日：1993-04-21

    申请人： Michael G. Murray ,  Jane H. Cramer ,  Jeanne Romero-Severson ,  David West ,  Yu Ma

    发明人： Michael G. Murray ,  Jane H. Cramer ,  Jeanne Romero-Severson ,  David West ,  Yu Ma

    IPC分类号： A01H5/10 ,  A01H1/00

    CPC分类号： A01H5/10

    摘要： A set of nucleic acid probes useful for tracking Maize Dwarf Mosaic Virus Resistance (MDMV), a polygenic trait, is provided. Chromosome segments are identified enabling isolation of the genes governing this trait.A general method for identifying probes useful for tracking and introgressing polygenic traits into elite genomes and identifying chromosome segments governing the traits is also provided. This method involves the analysis of RFLP polymorphisms between parent donor and recipient genotypes and observed phenotypic data using multiple regression by leaps and bounds ("leaps"), followed by standard multiple regression applied to the "leaps" data. The "leaps" data may be used to identify flanking markers, and epistasis may be determined by analysis of the multiple regression data.Kits comprising a subset of the most useful probes (those most closely linked to the trait of interest) are provided. The kits may also comprise flanking probes. Flanking probes used in combination with the most closely linked probes are useful in identifying situations in which donor DNA tends to move in clumps and recovering rare individuals in which traits of interest have separated from surrounding donor DNA, so that elite recipient DNA may be maximized.

    摘要翻译： 提供了一套用于跟踪玉米矮花叶病毒抗性（MDMV）的多核基因特征的核酸探针。 鉴定染色体片段能够分离控制该性状的基因。 还提供了用于鉴定用于跟踪和将多基因性状渗入精英基因组并鉴定控制性状的染色体片段的探针的一般方法。 该方法涉及使用多跳回归（“跨越”）分析父母供体和受体基因型之间的RFLP多态性，并使用多重回归（“跨越”）观察表型数据，然后应用于“跨越”数据的标准多元回归。 “跳跃”数据可以用于鉴定侧翼标记，并且可以通过分析多个回归数据来确定上位稳定性。 提供了包含最有用的探针子集（最感兴趣的特征）的试剂盒。 试剂盒还可以包含侧翼探针。 与最紧密连接的探针结合使用的侧翼探针可用于鉴定供体DNA倾向于移动并且回收其中感兴趣的性状与周围供体DNA分离的稀有个体的情况，从而可以使精英受体DNA最大化。

    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    2.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 有权
    用于植物基因工程的土地垫

    公开(公告)号：US20110191899A1

    公开(公告)日：2011-08-04

    申请号：US13011735

    申请日：2011-01-21

    申请人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebbeca R. Miles ,  Steven R. Webb

    发明人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebbeca R. Miles ,  Steven R. Webb

    IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

    摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    摘要翻译： 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    Engineered landing pads for gene targeting in plants
    5.
    发明授权
    Engineered landing pads for gene targeting in plants 有权
    用于植物中基因靶向的设计着陆垫

    公开(公告)号：US08802921B2

    公开(公告)日：2014-08-12

    申请号：US13011735

    申请日：2011-01-21

    申请人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebecca R. Miles ,  Steven R. Webb

    发明人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebecca R. Miles ,  Steven R. Webb

    IPC分类号： C12N15/87 ,  C12N15/82 ,  C12N15/05 ,  C12N5/04 ,  C12N5/10 ,  C07H21/04 ,  A01H5/10 ,  A01H5/00

    CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

    摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    摘要翻译： 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    Tailgate track mat
    8.
    发明授权
    Tailgate track mat 失效
    尾门轨道垫

    公开(公告)号：US4763945A

    公开(公告)日：1988-08-16

    申请号：US45471

    申请日：1987-05-04

    申请人： Michael G. Murray

    发明人： Michael G. Murray

    IPC分类号： B60R13/01 ,  B62D33/02

    CPC分类号： B60R13/01

    摘要： A tailgate track mat for preventing sand, dirt, gravel and other granular material from accumulating in the gap or track between the end of a truck bed and its tailgate, wherein the mat includes a resilient flexible strip of material extending over the track and transversely of the truck bed and tailgate, and having one edge fastened to the tailgate and the opposite edge engaging the bed of the truck.

    摘要翻译： 一种用于防止沙子，污垢，砾石和其它颗粒材料积聚在卡车床和其后挡板的端部之间的间隙或轨道中的后挡板轨道垫，其中所述垫包括弹性柔性带材，其延伸越过轨道并横向于 卡车底盘和后挡板，并且一个边缘固定在后挡板上，相对的边缘与卡车的底部接合。