公开(公告)号：US20240200021A1

公开(公告)日：2024-06-20

申请号：US18555663

申请日：2022-04-15

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene

IPC分类号： C12N1/20 ,  C12N1/18 ,  C12N9/10 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12N1/205 ,  C12N1/185 ,  C12N9/1081 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12N2500/34 ,  C12R2001/19

摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells of microorganisms and the use of said cells in a fermentation. The present invention describes a metabolically engineered cell of a microorganism and a method by fermentation with said cell for production of a sialylated di- and/or oligosaccharide. The metabolically engineered cell comprises a pathway for production of said sialylated di- and/or oligosaccharide, synthesizes sialic acid, expresses at least one sialyltransferase, preferably is modified in the expression or activity of at least one sialyltransferase, is modified to have a fully or partially knocked out or rendered less functional sialic acid catabolic pathway and is modified for overexpression of an endogenous sialic acid transporter and/or expression, preferably overexpression, of an exogenous, homologous and/or heterologous sialic acid transporter. Furthermore, the present invention provides for purification of said sialylated di- and/or oligosaccharide from the cultivation, preferably fermentation.

公开(公告)号：US20240084347A1

公开(公告)日：2024-03-14

申请号：US18261793

申请日：2022-01-17

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Sofie De Maeseneire ,  Yatti De Nijs ,  Tom Delmulle

IPC分类号： C12P19/12 ,  C12N9/10 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12N9/1096 ,  C12P19/18 ,  C12Y204/01222 ,  C12Y204/99003 ,  C12Y204/99004 ,  C12Y206/01016

摘要： This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of cultivation of metabolically engineered cells. This disclosure describes a method for the production of a glycosylated product derived from UDP-GlcNAc and comprising a di- or oligosaccharide that is composed of at least two different monosaccharide subunits by a cell as well as the separation of the glycosylated product from the cultivation. Furthermore, this disclosure provides a metabolically engineered cell for production of a glycosylated product derived from UDP-GlcNAc and comprising a di- or oligosaccharide that is composed of at least two different monosaccharide subunits. This disclosure also provides a cell excreting a di- or oligosaccharide out of the cell.

公开(公告)号：US20240076704A1

公开(公告)日：2024-03-07

申请号：US17767400

申请日：2020-10-14

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Nausicaä Lannoo ,  Gert Peters ,  Kristof Vandewalle ,  Annelies Vercauteren ,  Sofie Aesaert ,  Thomas Decoene

IPC分类号： C12P19/02 ,  C07K14/245 ,  C12N1/20 ,  C12N15/70 ,  C12P19/04 ,  C12P19/12 ,  C12P19/44

CPC分类号： C12P19/02 ,  C07K14/245 ,  C12N1/20 ,  C12N15/70 ,  C12P19/04 ,  C12P19/12 ,  C12P19/44 ,  C12R2001/19

摘要： Described is a method of producing bioproducts by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The cell is genetically modified to produce a bioproduct and is further genetically modified by reducing the expression of at least one endogenous membrane protein encoding gene and/or mutating the expression of the endogenous membrane protein.

公开(公告)号：US20240035055A1

公开(公告)日：2024-02-01

申请号：US18041054

申请日：2021-08-10

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Maarten Delforche ,  Gaspard Lequeux ,  Wim Soetaert ,  Thomas Verbrugghen

IPC分类号： C12P19/04 ,  B01D15/36 ,  C07H1/08 ,  B01D61/42 ,  B01D61/14 ,  B01D61/02

CPC分类号： C12P19/04 ,  B01D15/362 ,  C07H1/08 ,  B01D61/422 ,  B01D61/146 ,  B01D61/027

摘要： This disclosure is in the technical field of cell cultivation or fermentation for the production of oligosaccharides. The present application discloses a process for purification of an oligosaccharide solution produced by microbial fermentation.

公开(公告)号：US20230348944A1

公开(公告)日：2023-11-02

申请号：US17785843

申请日：2020-12-16

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Nausicaä Lannoo ,  Kristof Vandewalle ,  Annelies Vercauteren

IPC分类号： C12P19/18 ,  C12N9/10 ,  C12N1/20 ,  C12N15/70

CPC分类号： C12P19/18 ,  C12Y204/01069 ,  C12N9/1051 ,  C12N1/205 ,  C12N15/70 ,  C12R2001/19

摘要： The present invention relates to methods for producing 2′ fucosyllactose (2′-FL), as well as newly identified fucosyltransferases, more specifically newly identified lactose binding α-1,2-fucosyltransferase polypeptides, and their applications. Furthermore, the present invention provides methods for producing 2-fucosyllactose (2′FL) using the newly identified α-1,2-fucosyltransferases.

公开(公告)号：US20230313252A1

公开(公告)日：2023-10-05

申请号：US18041137

申请日：2021-08-10

申请人： INBIOSE N.V.

发明人： Sofie Aesaert ,  Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene ,  Nausicaä Lannoo ,  Gert Peters ,  Kristof Vandewalle

IPC分类号： C12P19/18

CPC分类号： C12P19/18

摘要： The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of metabolically engineered cells and use of the cells in a cultivation or fermentation. The disclosure describes a metabolically engineered cell and a method by cultivation or fermentation with the cell for production of a sialylated di- and/or oligosaccharide. The metabolically engineered cell comprises a pathway for production of the sialylated di- and/or oligosaccharide and is modified for expression and/or overexpression of multiple coding DNA sequences encoding one or more isoproteins that catalyze the same chemical reaction. Furthermore, the disclosure provides for purification of the sialylated di- and/or oligosaccharide from the cultivation.

公开(公告)号：US20220403431A1

公开(公告)日：2022-12-22

申请号：US17904213

申请日：2021-02-12

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Sofia De Maeseneire ,  Nico Snoeck

IPC分类号： C12P19/04 ,  C12P19/02 ,  C12N1/20 ,  C12N15/90

摘要： This disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria having a reduced or abolished synthesis of poly-N-acetyl-glucosamine (PNAG), Enterobacterial Common Antigen (ECA), cellulose, colanic acid, core oligosaccharides, Osmoregulated Periplasmic Glucans and Glucosylglycerol (O), glycan, and trebalose. The disclosure further provides methods for the production of bioproduct by the viable bacteria and uses thereof. Furthermore, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms producing bioproduct.

公开(公告)号：US10858684B2

公开(公告)日：2020-12-08

申请号：US15505809

申请日：2015-11-12

申请人： Inbiose N.V.

发明人： Joeri Beauprez ,  Sofie De Maeseneire ,  Eric Timmermans

IPC分类号： C12P19/12 ,  C07K14/245 ,  C12N15/52 ,  C12N15/10 ,  C12N15/81

摘要： The present invention relates to a method to produce mutated microorganisms which resist the phenomenon of lactose killing and to the microorganisms obtainable via said method. Such engineered microorganisms can be applied for the production of specialty products, such as but not limited to specialty carbohydrates, glycolipids and galactosylated compounds.

公开(公告)号：US20240294959A1

公开(公告)日：2024-09-05

申请号：US18555649

申请日：2022-04-15

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene ,  Annelies Vercauteren

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12N9/24 ,  C12P17/06

CPC分类号： C12P19/00 ,  C12N9/1081 ,  C12N9/1085 ,  C12N9/2402 ,  C12P17/06 ,  C12Y205/01056 ,  C12Y302/01183

摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells and use of said cells in a cultivation, preferably fermentation. The present invention describes a metabolically engineered cell and a method by cultivation, preferably fermentation, with said cell for production of a bioproduct. More specifically, the present invention describes a metabolically engineered cell and a method by cultivation, preferably fermentation, with said cell for production of an N-acetylneuraminic acid (Neu(n)Ac)-containing compound, wherein (n) is 4, 5, 7, 8 or 9 or a combination thereof. The metabolically engineered cell comprises a pathway for production of said Neu(n)Ac-containing compound and is modified in the expression or activity of at least one NeuNAc synthase according to the present invention. Furthermore, the present invention provides for purification of said Neu(n)Ac-containing compound from the cultivation.

公开(公告)号：US12077788B2

公开(公告)日：2024-09-03

申请号：US18040629

申请日：2021-08-10

申请人： INBIOSE N.V.

发明人： Sofie Aesaert ,  Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene ,  Nausicaä Lannoo ,  Gert Peters ,  Kristof Vandewalle ,  Annelies Vercauteren

IPC分类号： C12N9/10 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00 ,  C12N15/52 ,  C12N15/70 ,  C12P7/58 ,  C12P19/12 ,  C12P19/18 ,  C12P19/26

CPC分类号： C12N9/1048 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00 ,  C12N9/1029 ,  C12N9/1051 ,  C12N9/1096 ,  C12N15/52 ,  C12N15/70 ,  C12P7/58 ,  C12P19/12 ,  C12P19/18 ,  C12P19/26 ,  C12Y203/01004 ,  C12Y204/00 ,  C12Y206/01016 ,  C12N2500/34

摘要： The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of a mixture of at least four different neutral non-fucosylated oligosaccharides. Furthermore, the disclosure provides a method for the production of a mixture of at least four different neutral non-fucosylated oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation.