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公开(公告)号：US5386025A
公开(公告)日：1995-01-31
申请号：US482384
申请日：1990-02-20
IPC分类号： A61K38/00 , A61P43/00 , C07H21/02 , C07H21/04 , C07K14/00 , C07K14/705 , C07K19/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12R1/91
CPC分类号： C07K14/705 , C12Q1/6876
摘要： Calcium channel .gamma.-subunit-encoding cDNAs, and related compositions and methods, are provided.
摘要翻译： 提供钙通道γ-亚基编码cDNA，以及相关组合物和方法。
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公开(公告)号：US4808537A
公开(公告)日：1989-02-28
申请号：US666391
申请日：1984-10-30
申请人： David W. Stroman , Paul F. Brust , Steven B. Ellis , Thomas R. Gingeras , Michael M. Harpold , Juerg F. Tschopp
发明人： David W. Stroman , Paul F. Brust , Steven B. Ellis , Thomas R. Gingeras , Michael M. Harpold , Juerg F. Tschopp
CPC分类号： C12N15/815 , C12N15/52 , C12N15/68 , C12N15/70 , Y10S930/31
摘要： A method for isolating and cloning methanol inducible genes from Pichia is taught. The regulatory regions are useful for the methanol regulated expression of heterologous genes.
摘要翻译： 教导了从毕赤酵母中分离和克隆甲醇诱导型基因的方法。 调节区域对于异源基因的甲醇调节表达是有用的。
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23.发明授权Human neuronal nicotinic acetylcholine receptor compositions and methods employing same 失效人神经元烟碱乙酰胆碱受体组合物及其应用方法
公开(公告)号：US5910582A
公开(公告)日：1999-06-08
申请号：US700636
申请日：1996-07-16
IPC分类号： G01N33/50 , C07K14/705 , C07K14/715 , C07K16/28 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12P21/08 , C12Q1/00 , C12Q1/02 , C12Q1/68 , C12R1/91 , G01N33/15 , C07H21/02 , C07H21/04 , C12P21/00
CPC分类号： C07K14/70571
摘要： Nucleic acid encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically .alpha..sub.4 and .alpha..sub.7) and beta subunits (specifically .beta..sub.4) are provided. In addition, combinations of subunits (i.e., .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .alpha..sub.4, and/or .alpha..sub.7 subunits in combination with .beta..sub.4 subunits; or .beta..sub.2, .beta..sub.3 and/or .beta..sub.4 subunits in combination with .alpha..sub.4 and/or .alpha..sub.7 subunits) are provided.
摘要翻译： 编码人神经元烟碱乙酰胆碱受体α和β亚基的核酸，含有所述DNA的哺乳动物和两栖动物细胞，用于产生α和β亚基和重组（即，分离或基本上纯的）α亚基（特别是α4和α7）和β 亚单位（特别是beta 4）。 此外，亚单位（即α1，α2，α3，α4和/或α7亚基与β4亚基组合;或β2，β3和/或β4亚基与α组合） 4和/或α7亚基）。
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24.发明授权Human neuronal nicotinic acetylcholine receptor and cells transformed with same DNA and mRNA encoding an--subunit of 失效DNA和MRNA编码人类神经元烟碱乙酰胆碱受体的α7亚基和转化的细胞
公开(公告)号：US5837489A
公开(公告)日：1998-11-17
申请号：US466589
申请日：1995-06-05
IPC分类号： G01N33/50 , C07K14/705 , C07K14/715 , C07K16/28 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12P21/08 , C12Q1/00 , C12Q1/02 , C12Q1/68 , C12R1/91 , G01N33/15 , C12N15/00
CPC分类号： C07K14/70571
摘要： DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically .alpha..sub.4 and .alpha..sub.7) and beta subunits (specifically .beta..sub.4) are provided. In addition, combinations of subunits (i.e., .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .alpha..sub.4, and/or .alpha..sub.7 subunits in combination with .beta..sub.4 subunits; or .beta..sub.2, .beta..sub.3 and/or .beta..sub.4 subunits in combination with .alpha..sub.4 and/or .beta..sub.7 subunits) are provided.
摘要翻译： 编码人神经元烟碱乙酰胆碱受体α和β亚基的DNA，含有所述DNA的哺乳动物和两栖动物细胞，用于产生α和β亚基和重组（即，分离或基本上纯的）α亚基（特别是α4和α7）和β亚基的方法 （特别是beta 4）。 此外，亚单位（即α1，α2，α3，α4和/或α7亚基与β4亚基组合;或β2，β3和/或β4亚基与α组合） 4和/或β7亚基）。
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公开(公告)号：US5792846A
公开(公告)日：1998-08-11
申请号：US455543
申请日：1995-05-31
申请人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Daniel H. Feldman , Ann F. McCue , Robert Brenner
发明人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Daniel H. Feldman , Ann F. McCue , Robert Brenner
IPC分类号： A61K38/00 , C07K14/705 , C12N15/12 , C12Q1/68 , G01N33/564 , G01N33/68 , C07H21/04
CPC分类号： G01N33/6872 , C07K14/705 , C12Q1/6876 , C12Q1/6897 , G01N33/564 , G01N33/68 , G01N33/6893 , A61K38/00 , C12Q2600/158 , G01N2500/10
摘要： Isolated DNA encoding each of human calcium channel .alpha..sub.1 -, .alpha..sub.2 -, .beta.- and .gamma.-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.
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公开(公告)号：US5665600A
公开(公告)日：1997-09-09
申请号：US761528
申请日：1991-09-18
申请人： Mary Jane Hagenson , Kathryn A. Barr , David W. Stroman , Frank H. Gaertner , Michael M. Harpold , Ronald D. Klein
发明人： Mary Jane Hagenson , Kathryn A. Barr , David W. Stroman , Frank H. Gaertner , Michael M. Harpold , Ronald D. Klein
CPC分类号： C12N15/815 , Y10S435/938
摘要： Two novel linear DNA plasmids are described. Also, novel fragments of the plasmids containing the autonomous replication sequence (ARS), and thus capable of self-maintenance as extra chromosomal elements are provided. These novel DNA sequences of the present invention are useful in a vector for self-maintenance in Pichia pastoris and are also useful as plasmid vectors which can be shuttled between yeast and bacteria.
摘要翻译： 描述了两种新的线性DNA质粒。 此外，提供了含有自主复制序列（ARS）的质粒的新片段，因此能够作为额外的染色体元件进行自我维护。 本发明的这些新型DNA序列可用于在巴斯德毕赤酵母中进行自我维持的载体，并且也可用作能够在酵母和细菌之间穿梭的质粒载体。
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公开(公告)号：US4960690A
公开(公告)日：1990-10-02
申请号：US871111
申请日：1986-06-05
CPC分类号： C12Q1/6879 , C12Q1/6813 , Y10S436/814
摘要： Nucleic acid hybridizaiton probes are provided which have sequences complementary to sequences of segments in bovine male-specific DNA and are suitable for sexing bovine embryos at the time of embryo transfer with nearly 100% accuracy.
摘要翻译： 提供核酸杂交探针，其具有与牛雄性特异性DNA中的片段序列互补的序列，并且适于在胚胎移植时以近100％的准确性对牛胚胎进行性别化。
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公开(公告)号：US4895800A
公开(公告)日：1990-01-23
申请号：US801713
申请日：1985-11-26
IPC分类号： C12N15/09 , A61K39/29 , C07H21/04 , C07K14/02 , C12N1/16 , C12N1/19 , C12N15/00 , C12N15/51 , C12N15/81 , C12P21/00 , C12R1/84
CPC分类号： C07K14/005 , C12N15/815 , C12N2730/10122
摘要： Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon source starvation. The novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used to transform suitable hosts and ultimately used for the production and isolation of hepatitis B surface antigen in high yields.
摘要翻译： 公开了包含调节区加上乙型肝炎表面抗原结构编码区（HBsAg）的新型DNA构建体。 所采用的调节区域对甲醇，非分解代谢物抑制碳源和分解代谢物抑制碳源，随后碳源不足有反应。 将这些新型构建体并入多种线性和环状质粒。 这种质粒用于转化合适的宿主，最终以高产率用于生产和分离乙型肝炎表面抗原。
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公开(公告)号：US07414110B2
公开(公告)日：2008-08-19
申请号：US11702388
申请日：2007-02-05
申请人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Ann F. McCue , Alison Gillespie , Daniel H. Feldman , Robert Brenner
发明人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Ann F. McCue , Alison Gillespie , Daniel H. Feldman , Robert Brenner
IPC分类号： C07K14/435 , C07K14/705 , C12P21/02 , A61K38/17
CPC分类号： C07K14/705 , C12Q1/6876 , C12Q2600/158 , G01N33/564 , G01N33/68
摘要： Isolated DNA encoding each of human calcium channel α1-, α2-, β- and γ-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.
摘要翻译： 提供了编码人钙通道α1 - ，α2 - ，β-和γ-亚单位的分离的DNA，包括作为初级转录物的剪接变体产生的亚基 。 还提供了含有DNA的细胞和载体以及用于鉴定调节人钙通道活性的化合物的方法。
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公开(公告)号：US06653097B1
公开(公告)日：2003-11-25
申请号：US08450273
申请日：1995-05-25
申请人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Ann F. McCue , Alison Gillespie , Daniel H. Feldman , Robert Brenner
发明人： Michael M. Harpold , Steven B. Ellis , Mark E. Williams , Ann F. McCue , Alison Gillespie , Daniel H. Feldman , Robert Brenner
IPC分类号： C12N1500
CPC分类号： C07K14/705 , A61K38/00 , C12Q1/6876 , G01N33/564 , G01N33/68 , G01N33/6872 , G01N2500/10
摘要： Isolated DNA encoding each of human calcium channel &agr;1-, &agr;2-, &bgr;- and &ggr;-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.
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