公开(公告)号：US20150284727A1

公开(公告)日：2015-10-08

申请号：US14438098

申请日：2013-10-23

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim ,  Jong Min Kim ,  Seokjoong Kim

IPC: C12N15/52 ,  C12N9/16

CPC classification number: C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531 ,  C12Y301/21

Abstract: The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

Abstract translation: 本发明涉及真核细胞或生物体中靶向的基因组编辑。 更具体地说，本发明涉及用于在真核细胞或生物体中切割靶DNA的组合物，其包含对靶DNA和Cas蛋白质编码核酸或Cas蛋白质特异性的引导RNA及其用途。

公开(公告)号：US12286635B2

公开(公告)日：2025-04-29

申请号：US17706921

申请日：2022-03-29

Applicant: TOOLGEN INCORPORATED

Inventor： Seok Joong Kim ,  Ok Jae Koo ,  Min Hee Jung ,  Ye Seul Kim

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11

Abstract: The present invention relates to an artificially manipulated unsaturated fatty acid biosynthesis-associated factor and use thereof to increase the content of a specific unsaturated fatty acid of a plant body. More particularly, the present invention relates to a system capable of artificially controlling unsaturated fatty acid biosynthesis and a plant body produced thereby, which include an artificially manipulated unsaturated fatty acid biosynthesis-associated factor to control unsaturated fatty acid biosynthesis and a composition capable of artificially manipulating the factor. In a specific aspect, the present invention relates to artificially manipulated unsaturated fatty acid biosynthesis-associated factors such as FAD2, FAD3, FADE, FAD7 and FAD8 and/or an unsaturated fatty acid biosynthesis controlling system by an expression product thereof.

公开(公告)号：US20250051749A1

公开(公告)日：2025-02-13

申请号：US18723085

申请日：2022-12-23

Applicant: TOOLGEN INCORPORATED ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

Inventor： Su Cheong YEOM ,  Dong Woo SONG ,  Seokjoong KIM ,  Hye Kyung OH

IPC: C12N9/64 ,  C12N15/85

Abstract: The present application relates to a novel promoter and a use thereof. In one embodiment of the present application, provided is a nucleic acid construct comprising a novel promoter and a transgene of the present application. At this time, on the nucleic acid construct, the novel promoter of the present application can be operably linked to the transgene.

公开(公告)号：US20240026367A1

公开(公告)日：2024-01-25

申请号：US18467967

申请日：2023-09-15

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/52 ,  C12N15/82 ,  C12N15/63 ,  C12N9/16 ,  C12N15/11 ,  C12N15/10 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22

CPC classification number: C12N15/52 ,  C12N15/8216 ,  C12N15/63 ,  C12N9/16 ,  C12N15/111 ,  C12N15/102 ,  C12N15/907 ,  C12N15/85 ,  C12N9/22 ,  C12Y301/21 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides compositions for inducing targeted disruption of endogenous genes in eukaryotic cells. The composition may comprise a Cas9/guide RNA complex with a Cas9 protein to which a NLS is linked, and a guide RNA having a crRNA and a tracrRNA. The crRNA may comprise i) a portion to be hybridized with a portion of the tracrRNA, and ii) a portion complementary to a target DNA of the endogenous genes. In some embodiments, the Cas/guide RNA complex may be formed in vitro before being introduced into the eukaryotic cell.

公开(公告)号：US20230293645A1

公开(公告)日：2023-09-21

申请号：US17604498

申请日：2020-04-20

Applicant: TOOLGEN INCORPORATED ,  HANDONG GLOBAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION ,  KONKUK UNIVERSITY GLOCAL INDUSTRY-ACADEMIC COLLABORATION FOUNDATION

Inventor： Jae Young LEE ,  Kyu Jun LEE ,  Jung Min LEE ,  Kyun Hwan KIM ,  Ju Hee WON

IPC: A61K38/46 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85 ,  C12N15/88 ,  C12N5/071 ,  A61K31/7105 ,  A61P31/20

CPC classification number: A61K38/465 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85 ,  C12N15/88 ,  C12N5/067 ,  A61K31/7105 ,  A61P31/20 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2800/107

Abstract: The present application relates to a composition and method for inhibiting Hepatitis B virus (HBV) proliferation.

公开(公告)号：US20230287461A1

公开(公告)日：2023-09-14

申请号：US18139481

申请日：2023-04-26

Applicant: TOOLGEN INCORPORATED

Inventor： Seokjoong KIM ,  Dong Woo SONG ,  Kyu Jun LEE ,  Jung-Min LEE ,  Un-Gi KIM

IPC: C12N15/90 ,  C12N9/22 ,  C12N9/64 ,  C12N15/86 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N9/644 ,  C12N15/86 ,  C12N15/11 ,  C12N2800/80 ,  C12N2310/20 ,  C12N2750/14143

Abstract: Provided is a platform for expressing a protein of interest by artificially manipulating the liver, and more particularly, to a platform for alleviating or treating a genetic disorder or improving a body function by inducing expression by inserting a transgene (e.g., a therapeutic gene) which can function or be expressed normally, into a high-expression secretory gene, instead of a disease gene which functions or is expressed abnormally. The high-expression secretory gene includes the HP or APOC3 gene. The transgene includes one that is highly expressed using a promoter in a hepatocyte genome and is secretory out of the cell.

公开(公告)号：US11667934B2

公开(公告)日：2023-06-06

申请号：US16623017

申请日：2018-06-15

Applicant: TOOLGEN INCORPORATED

Inventor： Seokjoong Kim ,  Dong Woo Song ,  Kyu Jun Lee ,  Jung-Min Lee ,  Un-Gi Kim

IPC: C12N15/90 ,  C12N9/22 ,  C12N9/64 ,  C12N15/11 ,  C12N15/86 ,  A61K38/17 ,  A61P7/04 ,  A61K48/00 ,  C07K14/775 ,  C12N15/113

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N9/644 ,  C12N15/11 ,  C12N15/86 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2800/80

Abstract: Provided is a platform for expressing a protein of interest by artificially manipulating the liver, and more particularly, to a platform for alleviating or treating a genetic disorder or improving a body function by inducing expression by inserting a transgene (e.g., a therapeutic gene) which can function or be expressed normally, into a high-expression secretory gene, instead of a disease gene which functions or is expressed abnormally. The high-expression secretory gene includes the HP or APOC3 gene. The transgene includes one that is highly expressed using a promoter in a hepatocyte genome and is secretory out of the cell.

公开(公告)号：US11572574B2

公开(公告)日：2023-02-07

申请号：US16776707

申请日：2020-01-30

Applicant: TOOLGEN INCORPORATED

Inventor： Seokjoong Kim ,  Dong Woo Song ,  Jae Young Lee ,  Jung Min Lee ,  Gyu-bon Cho ,  Hee Sook Bae

IPC: C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N15/113 ,  C12N9/22

Abstract: The present invention relates to an expression control composition for controlling the expression of a duplicate gene or a method using the same. In addition, the present invention relates to a method of treating or improving a disease caused by gene duplication using the expression control composition for controlling the expression of a duplicate gene.

公开(公告)号：US20210095316A1

公开(公告)日：2021-04-01

申请号：US16623017

申请日：2018-06-15

Applicant: TOOLGEN INCORPORATED

Inventor： Seokjoong KIM ,  Dong Woo SONG ,  Kyu Jun LEE ,  Jung-Min LEE ,  Un-Gi KIM

IPC: C12N15/90 ,  C12N9/64 ,  C12N15/11 ,  C12N9/22 ,  C12N15/86

Abstract: Provided is a platform for expressing a protein of interest by artificially manipulating the liver, and more particularly, to a platform for alleviating or treating a genetic disorder or improving a body function by inducing expression by inserting a transgene (e.g., a therapeutic gene) which can function or be expressed normally, into a high-expression secretory gene, instead of a disease gene which functions or is expressed abnormally. The high-expression secretory gene includes the HP or APOC3 gene. The transgene includes one that is highly expressed using a promoter in a hepatocyte genome and is secretory out of the cell.

公开(公告)号：US20200208166A1

公开(公告)日：2020-07-02

申请号：US16471443

申请日：2017-09-26

Applicant: TOOLGEN INCORPORATED

Inventor： Seok Joong KIM ,  Ok Jae KOO ,  Min Hee JUNG ,  Ye Seul KIM

IPC: C12N15/82 ,  C12N15/11 ,  C12N9/22

Abstract: The present invention relates to an artificially manipulated unsaturated fatty acid biosynthesis-associated factor and use thereof to increase the content of a specific unsaturated fatty acid of a plant body. More particularly, the present invention relates to a system capable of artificially controlling unsaturated fatty acid biosynthesis and a plant body produced thereby, which include an artificially manipulated unsaturated fatty acid biosynthesis-associated factor to control unsaturated fatty acid biosynthesis and a composition capable of artificially manipulating the factor. In a specific aspect, the present invention relates to artificially manipulated unsaturated fatty acid biosynthesis-associated factors such as FAD2, FAD3, FADE, FAD7 and FAD8 and/or an unsaturated fatty acid biosynthesis controlling system by an expression product thereof.