公开(公告)号：US09115177B2

公开(公告)日：2015-08-25

申请号：US14116947

申请日：2012-05-11

申请人： Guangjun Jiao ,  Shidong Liu ,  Bingming He ,  Zhijun Tang ,  Xiaoming Ji

发明人： Guangjun Jiao ,  Shidong Liu ,  Bingming He ,  Zhijun Tang ,  Xiaoming Ji

IPC分类号： C07K1/113 ,  C07K7/56 ,  C07K1/14 ,  C07D413/12

CPC分类号： C07K1/14 ,  C07D413/12 ,  C07K1/113 ,  C07K7/56

摘要： The method of the preparation of micafungin sodium comprises the step of mixing the weak base and the aqueous solution containing micafungin acid (the structure is illustrated by formula I) or the mixed aqueous solution containing the compound of formula I and organic solvent in order to obtain the sodium salt of micafungin (the structure is illustrated by formula II).

摘要翻译： 咪达能素钠的制备方法包括将弱碱与含有米怕酸的水溶液（式I所示的结构）或含有式I化合物和有机溶剂的混合水溶液混合的步骤，以获得 米卡芬净的钠盐（结构由式II表示）。

公开(公告)号：US20130338060A1

公开(公告)日：2013-12-19

申请号：US13982874

申请日：2012-01-31

申请人： Yunhai Hong ,  Ying Xue ,  Xiaoming Ji

发明人： Yunhai Hong ,  Ying Xue ,  Xiaoming Ji

IPC分类号： A61K47/26

CPC分类号： A61K47/26 ,  A61K9/0019 ,  A61K31/42 ,  A61K38/12

摘要： The present invention provides a liquid medicinal composition containing micafungin or a pharmaceutically acceptable salt thereof and a stabilizing agent.

摘要翻译： 本发明提供了含有微风孔蛋白或其药学上可接受的盐和稳定剂的液体药物组合物。

公开(公告)号：US20130281665A1

公开(公告)日：2013-10-24

申请号：US13877427

申请日：2011-09-27

申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

IPC分类号： C07K1/22

CPC分类号： C07K1/22 ,  C07K7/56

摘要： A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.

摘要翻译： 一种用于纯化环状脂肽化合物或其盐的方法，包括以下步骤：（1）将式I的粗制化合物装入大孔吸附树脂中; （2）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗涤液洗涤大孔吸附树脂; 和（3）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗脱剂，从大孔吸附树脂中洗脱式I化合物。 净化方法的优点是使用少量的有机溶剂，不使用硅胶，对环境几乎没有损害; 与以前公开的方法相比，所收集的式I化合物的纯度也得到改善。

公开(公告)号：US07582464B2

公开(公告)日：2009-09-01

申请号：US11328494

申请日：2006-01-06

申请人： Mingquan Mei ,  Xiaoming Ji ,  Xiaoliang Gao ,  Yi Chen ,  Yan Li ,  Yong Yao ,  Zhonghao Zhuo ,  Jing Xu

发明人： Mingquan Mei ,  Xiaoming Ji ,  Xiaoliang Gao ,  Yi Chen ,  Yan Li ,  Yong Yao ,  Zhonghao Zhuo ,  Jing Xu

IPC分类号： C12N1/20

CPC分类号： C12P7/42 ,  C12P17/06 ,  C12R1/01 ,  C12R1/365

摘要： The invention provides a novel microorganism producing pravastatin sodium, as well as the method for producing pravastatin sodium by using this microorganism. Micropolyspora roseoalba CGMCC 0624 of the invention is highly tolerant to mevastatin sodium, and has a high transformation rate of mevastatin sodium, and can produce pravastatin sodium with a high efficiency and low cost.

摘要翻译： 本发明提供了一种生产普伐他汀钠的新型微生物，以及通过使用该微生物生产普伐他汀钠的方法。 本发明的微孢子虫多糖CGMCC 0624对美伐他汀钠具有高度耐受性，并且具有高的美伐他汀钠转化率，并且可以以高效率和低成本生产普伐他汀钠。

公开(公告)号：US09446091B2

公开(公告)日：2016-09-20

申请号：US14113296

申请日：2012-04-20

申请人： Bingming He ,  Ming Li ,  Zhijun Tang ,  Xiaoming Ji

发明人： Bingming He ,  Ming Li ,  Zhijun Tang ,  Xiaoming Ji

IPC分类号： C07K7/56 ,  A61K38/12 ,  A61K38/00 ,  C07K7/54 ,  A61K31/19

CPC分类号： A61K38/12 ,  A61K31/19 ,  A61K38/005 ,  C07K7/54 ,  C07K7/56

摘要： Disclosed are a high purity of caspofungin or salts thereof, and a preparation method thereof, and use thereof. Disclosed are a caspofungin or salts thereof with low solvent residue and hyposaline, and a preparation process comprising: dissolving a crude product of caspofungin or salts thereof into a system of water and acetic acid, then mixing with a first organic solvent ethyl alcohol, subsequently mixing with a second organic solvent ethyl acetate, then being subject to filtration and drying together with water, to obtain caspofungin or salts thereof with high stability, low solvent residue and hyposaline.

摘要翻译： 公开了高纯度的卡泊芬净或其盐，及其制备方法及其用途。 公开了具有低溶剂残留和次高效的卡泊芬净或其盐，以及制备方法，包括：将卡泊芬净或其盐的粗产物溶解在水和乙酸的体系中，然后与第一有机溶剂乙醇混合，随后混合 用第二有机溶剂乙酸乙酯，然后与水一起过滤和干燥，得到高稳定性，低溶剂残留和次高效的卡泊芬净或其盐。

公开(公告)号：US08927690B2

公开(公告)日：2015-01-06

申请号：US13877427

申请日：2011-09-27

申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

IPC分类号： A61K38/00 ,  C07K1/00 ,  C07K1/22 ,  C07K7/56

CPC分类号： C07K1/22 ,  C07K7/56

摘要： A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.

摘要翻译： 一种用于纯化环状脂肽化合物或其盐的方法，包括以下步骤：（1）将式I的粗制化合物装入大孔吸附树脂中; （2）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗涤液洗涤大孔吸附树脂; 和（3）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗脱剂，从大孔吸附树脂中洗脱式I化合物。 净化方法的优点是使用少量的有机溶剂，不使用硅胶，对环境几乎没有损害; 与以前公开的方法相比，所收集的式I化合物的纯度也得到改善。

公开(公告)号：US08911968B2

公开(公告)日：2014-12-16

申请号：US13994633

申请日：2011-12-15

申请人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

发明人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

IPC分类号： C12P21/04 ,  C07K7/54 ,  C12R1/645 ,  C12N15/01 ,  C12N1/14 ,  C07K7/56

CPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12N15/01 ,  C12R1/645

摘要： High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

摘要翻译： 本发明提供了高产抗生素生产菌株，其制备方法和用途。 高产菌株是来自科氏白菜的诱变菌株，保藏在CGMCC的CGMCC中，登录号为CGMCC 4129.该制备方法包括以下步骤：（a）将加入号为FERM BP的Colephoma empetri种子液 -2635与亚硝基胍混合得到混合物; （b）将所述混合物a与破壁酶混合以获得原生质体; （c）再生所述原生质体以获得单个菌落; 和（d）培养所述单个菌​​落以获得所述诱变菌株。 得到的菌株具有稳定的遗传和生产特性，发酵中杂质少，适用于工业化。

公开(公告)号：US20140162314A1

公开(公告)日：2014-06-12

申请号：US13994633

申请日：2011-12-15

申请人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

发明人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

IPC分类号： C07K7/54

CPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12N15/01 ,  C12R1/645

摘要： High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

摘要翻译： 本发明提供了高产抗生素生产菌株，其制备方法和用途。 高产菌株是来自科氏白菜的诱变菌株，保藏在CGMCC的CGMCC中，登录号为CGMCC 4129.该制备方法包括以下步骤：（a）将加入号为FERM BP的Colephoma empetri种子液 -2635与亚硝基胍混合得到混合物; （b）将所述混合物a与破壁酶混合以获得原生质体; （c）再生所述原生质体以获得单个菌落; 和（d）培养所述单个菌​​落以获得所述诱变菌株。 得到的菌株具有稳定的遗传和生产特性，发酵中杂质少，适用于工业化。

公开(公告)号：US08664275B2

公开(公告)日：2014-03-04

申请号：US13505360

申请日：2010-07-30

申请人： Bingming He ,  Zhijun Tang ,  Zhonghao Zhuo ,  Yunman Zheng ,  Xiaoliang Gao ,  Xiaoming Ji

发明人： Bingming He ,  Zhijun Tang ,  Zhonghao Zhuo ,  Yunman Zheng ,  Xiaoliang Gao ,  Xiaoming Ji

IPC分类号： A61K31/165 ,  C07C235/34

CPC分类号： C07C405/00 ,  C07B2200/13 ,  C07C2601/08

摘要： The crystalline form A of Bimatoprost of formula I, its preparation method and use are provided. There are characteristic peaks where diffraction angles 2θ are 3.2±0.2°, 5.5±0.2°, 11.4±0.2°, 16.7±0.2°, 17.6±0.2°, 19.9±0.2°, 20.8±02° and 22.8±0.2° in the X-ray powder diffraction pattern of the crystalline form A.

摘要翻译： 提供了式I的比马前列素的结晶形式A及其制备方法和用途。 有衍射角2θ为3.2±0.2°，5.5±0.2°，11.4±0.2°，16.7±0.2°，17.6±0.2°，19.9±0.2°，20.8±02°和22.8±0.2°的特征峰 晶型A的X射线粉末衍射图

公开(公告)号：US20130296229A1

公开(公告)日：2013-11-07

申请号：US13884750

申请日：2011-11-10

申请人： Bingming He ,  Ming Li ,  Zhijun Tang ,  Xiaoming Ji

发明人： Bingming He ,  Ming Li ,  Zhijun Tang ,  Xiaoming Ji

IPC分类号： C07K7/56

CPC分类号： C07K7/56 ,  A61K38/00 ,  A61K38/12

摘要： Disclosed are a caspofungin analog and applications thereof. Said caspofungin analog is a compound having a structure as indicated in Formula (4), or pharmaceutically acceptable slats thereof. R1 can be chosen from hydroxyl, benzyloxy, phenoxy, substituted phenoxy, or substituted benzyloxy. R2, R3, R4, R5 can be chosen from hydrogen, C1-C6 alkyl, C1-C6 alkoxy, hydroxyl, benzyloxyphenyl, substituted benzyloxyphenyl, nitro, fluorine, chlorine, bromine, or iodine. Also disclosed are a preparation method for and applications of said compound.

摘要翻译： 公开了卡泊芬净类似物及其应用。 所述卡泊芬净类似物是具有式（4）所示结构的化合物或其药学上可接受的盐条。 R 1可以选自羟基，苄氧基，苯氧基，取代的苯氧基或取代的苄氧基。 R2，R3，R4，R5可以选自氢，C1-C6烷基，C1-C6烷氧基，羟基，苄氧基苯基，取代的苄氧基苯基，硝基，氟，氯，溴或碘。 还公开了所述化合物的制备方法和应用。