公开(公告)号：US20110059442A1

公开(公告)日：2011-03-10

申请号：US12660524

申请日：2010-02-26

申请人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

发明人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2565/543 ,  C12Q2525/313

摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.

公开(公告)号：US20090298709A1

公开(公告)日：2009-12-03

申请号：US12454746

申请日：2009-05-21

申请人： Yunqing Ma

发明人： Yunqing Ma

IPC分类号： C40B30/04 ,  C12Q1/68

CPC分类号： C12Q1/682 ,  C12Q2565/519 ,  C12Q2525/151 ,  C12Q2525/313

摘要： Methods of detecting copy number of a repeated sequence element, including methods of determining telomere length, are provided. The methods can be multiplexed for detection of repeated sequence element copy number on two or more nucleic acid targets simultaneously. Compositions, kits, and systems related to the methods are also described.

摘要翻译： 提供了检测重复序列元件的拷贝数的方法，包括确定端粒长度的方法。 所述方法可以多路复用以同时检测两个或多个核酸靶上的重复序列元件拷贝数。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US20070015188A1

公开(公告)日：2007-01-18

申请号：US11471025

申请日：2006-06-19

申请人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

发明人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2565/543 ,  C12Q2525/313

摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.

摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US20120003648A1

公开(公告)日：2012-01-05

申请号：US13175393

申请日：2011-07-01

申请人： Yunqing Ma ,  Gary K. McMaster ,  Aiguo Zhang ,  Quan N. Nguyen

发明人： Yunqing Ma ,  Gary K. McMaster ,  Aiguo Zhang ,  Quan N. Nguyen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2525/313 ,  C12Q2525/113

摘要： Disclosed are methods, compositions and kits for amplifying signals for detecting the presence, quantity and/or sequence of nucleic acids and proteins, as well as methods, compositions and kits for increasing the number of such targets simultaneously detectable in a sample. Detection may be, for instance, in vivo, in cellulo or in situ. Amplification of signal is achieved by way of hybridization of nucleic acid label probe systems and structures. Increase in target multiplex capacity is achieved by way of varying the type of labels utilized in the nucleic acid label probe system.

摘要翻译： 公开了用于扩增用于检测核酸和蛋白质的存在，数量和/或序列的信号的方法，组合物和试剂盒，以及用于增加样品中同时可检测的这些靶标数量的方法，组合物和试剂盒。 检测可以是例如体内，纤维素或原位。 通过核酸标记探针系统和结构的杂交实现信号的扩增。 通过改变在核酸标记探针系统中使用的标记类型来实现目标多重容量的增加。

公开(公告)号：US07951539B2

公开(公告)日：2011-05-31

申请号：US12587523

申请日：2009-10-07

申请人： Gary McMaster ,  Yunqing Ma

发明人： Gary McMaster ,  Yunqing Ma

IPC分类号： C12Q1/68

CPC分类号： C12Q1/682 ,  C12Q1/6837 ,  C12Q2563/103 ,  C12Q2537/162 ,  C12Q2531/113

摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.

摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。

公开(公告)号：US07709198B2

公开(公告)日：2010-05-04

申请号：US11471025

申请日：2006-06-19

申请人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

发明人： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2565/543 ,  C12Q2525/313

摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.

摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US20100099175A1

公开(公告)日：2010-04-22

申请号：US12587523

申请日：2009-10-07

申请人： Gary McMaster ,  Yunqing Ma

发明人： Gary McMaster ,  Yunqing Ma

IPC分类号： C12M1/34

CPC分类号： C12Q1/682 ,  C12Q1/6837 ,  C12Q2563/103 ,  C12Q2537/162 ,  C12Q2531/113

摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.

摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。

公开(公告)号：US07615351B2

公开(公告)日：2009-11-10

申请号：US11983216

申请日：2007-11-06

申请人： Gary McMaster ,  Yunqing Ma

发明人： Gary McMaster ,  Yunqing Ma

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/682 ,  C12Q1/6837 ,  C12Q2563/103 ,  C12Q2537/162 ,  C12Q2531/113

摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.

摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。

公开(公告)号：US20080050746A1

公开(公告)日：2008-02-28

申请号：US11893356

申请日：2007-08-14

申请人： Gary McMaster ,  Joan Davies ,  Yunqing Ma ,  Yuling Luo

发明人： Gary McMaster ,  Joan Davies ,  Yunqing Ma ,  Yuling Luo

IPC分类号： C12N5/02 ,  C12Q1/68

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6841 ,  Y10T436/143333 ,  C12Q2527/101 ,  C12Q2523/32 ,  C12Q2521/537 ,  C12Q2545/101

摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.

摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。