公开(公告)号：US12264316B2

公开(公告)日：2025-04-01

申请号：US16419428

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20250027149A1

公开(公告)日：2025-01-23

申请号：US18760903

申请日：2024-07-01

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US12117378B2

公开(公告)日：2024-10-15

申请号：US18205397

申请日：2023-06-02

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Andrew D. Price ,  Michael Schnall-Levin

IPC: G01N1/28 ,  C12N5/00 ,  G01N1/40

CPC classification number: G01N1/28 ,  C12N5/0006 ,  G01N1/4077 ,  C12N2537/10

Abstract: Methods and systems for cell and bead processing or analysis are provided. A cell or bead can be subjected to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). The cell or bead can further be subjected to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks are formed within the cell or bead.

公开(公告)号：US20240326052A1

公开(公告)日：2024-10-03

申请号：US18738471

申请日：2024-06-10

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. MASQUELIER ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US12084716B2

公开(公告)日：2024-09-10

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US11713457B2

公开(公告)日：2023-08-01

申请号：US17986764

申请日：2022-11-14

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

CPC classification number: C12N15/1065 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B20/04 ,  C40B50/16 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11629344B2

公开(公告)日：2023-04-18

申请号：US17860880

申请日：2022-07-08

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11193122B2

公开(公告)日：2021-12-07

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210222158A1

公开(公告)日：2021-07-22

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210040535A1

公开(公告)日：2021-02-11

申请号：US16915493

申请日：2020-06-29

Applicant: 10X GENOMICS, INC.

Inventor： Adam Lowe ,  Christopher Hindson ,  Andrew D. Price

IPC: C12Q1/6806 ,  C12N15/10 ,  B01J19/00 ,  B01J13/04

Abstract: The present disclosure provides particles (e.g., beads) and methods, kits, and systems involving the same for sample processing or analysis. Such particles may include one or more analytes, one or more reagents, and two or more gel components and/or walled components. The particles described herein may be formed, for example, by polymerization of a polymerizable material in proximity to a gel or walled component.