摘要:
A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.
摘要:
The invention provides nucleotide sequences from Coryneform bacteria coding for the ChrS protein and a process for the fermentative preparation of amino acids using bacteria in which the ChrS protein is attenuated.
摘要:
The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
摘要:
A method for the fermentative preparation of D-pantothenic acid and/or its salts or feedstuff additives containing these. Said method comprising, fermentation of Escherichia coli microorganisms wherein the nucleotide sequence(s) coding the glyA gene is overexpressed.
摘要:
The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.
摘要翻译:本发明涉及含有多核苷酸序列的多核苷酸序列,该多核苷酸序列选自a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸,b)编码 对于含有与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列的多肽,c)与a)或b)的多核苷酸互补的多核苷酸,以及d)至少含有 a),b)或c)的多核苷酸序列的15个连续核苷酸,以及通过发酵使用其中至少有citE基因以减毒形式存在的棒状细菌产生L-氨基酸的方法, 使用含有本发明序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
摘要翻译:本发明涉及一种分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID NO:2的氨基酸序列的多肽的至少70%的多核苷酸程度相同 b)编码多肽的多核苷酸,其包含与SEQ ID NO:2的氨基酸序列至少70%的程度相同的氨基酸序列)与a)和b的多核苷酸互补的多核苷酸 )和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。
摘要:
The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the carbon starvation protein A (cstA) gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in enhanced form and a vector which carries at least the cstA gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码碳饥饿蛋白A(cstA)基因的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸,以及具有其中cstA基因以增强形式存在的棒状细胞宿主细菌的宿主 - 载体系统和 携带至少根据SEQ ID NO:1的cstA基因的载体,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the cstA gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in attenuated form and a vector which carries at least the cstA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码cstA基因的多核苷酸序列的分离的多核苷酸,以及具有cstA基因以减毒形式存在的棒状细胞宿主细菌的宿主载体系和至少携带cstA基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to polynucleotides corresponding to the oxyR gene from Corynebacterium glutamicum and which encode a OxyR transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having OxyR transcriptional regulator activity.