公开(公告)号：US20140200162A1

公开(公告)日：2014-07-17

申请号：US14233256

申请日：2012-05-16

申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q2525/207 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/143 ,  C12Q2563/149

摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

公开(公告)号：US20050271828A1

公开(公告)日：2005-12-08

申请号：US11185897

申请日：2005-07-21

申请人： Toshiro Saito ,  Haruo Akahoshi ,  Takeyuki Itabashi

发明人： Toshiro Saito ,  Haruo Akahoshi ,  Takeyuki Itabashi

IPC分类号： H05K3/18 ,  H01L21/48 ,  H05H1/00 ,  H05K1/03 ,  H05K3/38 ,  H05K3/46

CPC分类号： H01L21/4846 ,  H01L2224/16225 ,  H01L2924/00014 ,  H05K3/381 ,  H05K3/389 ,  H05K3/4623 ,  H05K3/4661 ,  H05K2201/0344 ,  H05K2203/0315 ,  H05K2203/0793 ,  H05K2203/095 ,  Y10T29/49126 ,  H01L2224/0401

摘要： Provided is a wiring board and production method thereof, wherein production of wiring by a full additive method is achieved. This is extremely useful in forming fine copper wiring featuring a high adhesion on an insulating resin substrate. A resin having an excellent alkali resistance is used as the insulating resin substrate, and the copper wiring is formed on the insulating resin substrate through a degenerated layer containing amide group and a metallic oxide layer of a metal having a reduction potential more base than that of copper. The degenerated layer can be provided by, e.g., introduction of amide group into the surface of the insulating resin substrate. The copper can be formed by processes including electroless plating. The insulating resin substrate has superb heat resistance and dimensional stability, and the formed structure can provide a highly packed wiring board.

摘要翻译： 本发明提供一种布线基板及其制造方法，其特征在于，通过全加法制造布线。 这对于在绝缘树脂基板上形成具有高粘附性的细铜布线非常有用。 使用耐碱性优异的树脂作为绝缘树脂基板，并且通过包含酰胺基的退化层和金属氧化物层，在绝缘树脂基板上形成铜布线，该金属氧化物层的还原电位比 铜。 退化层可以通过例如将酰胺基引入绝缘树脂基材的表面来提供。 铜可以通过包括无电镀的工艺形成。 绝缘树脂基板具有极好的耐热性和尺寸稳定性，并且所形成的结构可以提供高度封装的布线板。

公开(公告)号：US20050208561A1

公开(公告)日：2005-09-22

申请号：US11113195

申请日：2005-04-25

申请人： Kazuhito Rokutan ,  Hiroyuki Tomita ,  Toshiro Saito

发明人： Kazuhito Rokutan ,  Hiroyuki Tomita ,  Toshiro Saito

IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N37/00 ,  C12M1/34

CPC分类号： G06F19/20 ,  C12Q1/6883 ,  C12Q2565/513 ,  C12Q2600/158 ,  C12Q2600/166

摘要： An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.

摘要翻译： 一种寡核苷酸阵列，其包含具有不同碱基序列的多个寡核苷酸阵列，其固定在载体底物上的已知和分开的位置上，其中所述寡核苷酸是与所述基因相关的生物应激相关基因或互补序列链，并且所述寡核苷酸根据它们 基因功能，其中支撑衬底上的固定区域被划分为所述分类。

公开(公告)号：US20050164253A1

公开(公告)日：2005-07-28

申请号：US11000924

申请日：2004-12-02

申请人： Takashi Yamamura ,  Junichi Satoh ,  Toshiro Saito

发明人： Takashi Yamamura ,  Junichi Satoh ,  Toshiro Saito

IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： This invention provides a method and a means for assisting in the diagnosis of multiple sclerosis. More particularly, this invention provides gene markers (shown in Tables 1 and 2) for evaluating whether or not multiple sclerosis has been developed, a method for evaluating multiple sclerosis using such gene markers, a chip, and the like.

摘要翻译： 本发明提供了一种辅助诊断多发性硬化症的方法和手段。 更具体地，本发明提供用于评估是否已经开发多发性硬化症的基因标记（示于表1和表2），使用这种基因标记评估多发性硬化的方法，芯片等。

公开(公告)号：US10294519B2

公开(公告)日：2019-05-21

申请号：US14233256

申请日：2012-05-16

申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC分类号： C12Q1/6834 ,  C12N15/10

摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

公开(公告)号：US08865403B2

公开(公告)日：2014-10-21

申请号：US12997469

申请日：2009-05-13

申请人： Masatoshi Narahara ,  Toshiro Saito ,  Naoshi Itabashi ,  Jiro Yamamoto ,  Hiroyuki Uchiyama

发明人： Masatoshi Narahara ,  Toshiro Saito ,  Naoshi Itabashi ,  Jiro Yamamoto ,  Hiroyuki Uchiyama

IPC分类号： C12Q1/68 ,  C12M1/36 ,  G01N21/00 ,  G01N21/64

CPC分类号： G01N21/648

摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.

摘要翻译： 本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。 本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为 生成。 根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的 附着有荧光团的核苷酸。

公开(公告)号：US20120316087A1

公开(公告)日：2012-12-13

申请号：US13575564

申请日：2010-12-01

申请人： Yoshiaki Sugimura ,  Masatoshi Narahara ,  Kazumichi Imai ,  Toshiro Saito ,  Ryoji Inaba ,  Takuya Matsui

发明人： Yoshiaki Sugimura ,  Masatoshi Narahara ,  Kazumichi Imai ,  Toshiro Saito ,  Ryoji Inaba ,  Takuya Matsui

IPC分类号： C40B60/10

CPC分类号： G01N33/54313 ,  B01L3/502715 ,  B01L2200/0668 ,  B01L2300/0609 ,  B01L2300/0877 ,  B01L2300/0883 ,  G01N21/6452 ,  G01N21/648 ,  G01N33/5308 ,  G01N33/54373 ,  G01N33/582 ,  G01N35/00 ,  G01N2035/00564

摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).

摘要翻译： 提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。 通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。 携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

公开(公告)号：US20090079988A1

公开(公告)日：2009-03-26

申请号：US12233184

申请日：2008-09-18

申请人： Masatoshi Narahara ,  Satoshi Takahashi ,  Toshiro Saito

发明人： Masatoshi Narahara ,  Satoshi Takahashi ,  Toshiro Saito

IPC分类号： G01N21/55 ,  G01J1/58

CPC分类号： G01N21/648 ,  G01N21/6428

摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.

摘要翻译： 本发明的目的是检测具有高对比度的目标物质。 本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率 指数比底物。 从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。 检测由目标物质通过ev逝光的等离子体共振产生的光。 根据本发明，可以减少对目标物质以外的其他武器的ev逝光的照射，从而可以减少来自与目标物质以外的武装物（例如，悬浮在目标物质周围的分子）的发光。

公开(公告)号：US06495769B1

公开(公告)日：2002-12-17

申请号：US09536645

申请日：2000-03-28

申请人： Toshiro Saito ,  Haruo Akahoshi ,  Takeyuki Itabashi

发明人： Toshiro Saito ,  Haruo Akahoshi ,  Takeyuki Itabashi

IPC分类号： H05K103

CPC分类号： H01L21/4846 ,  H01L2224/16225 ,  H01L2924/00014 ,  H05K3/381 ,  H05K3/389 ,  H05K3/4623 ,  H05K3/4661 ,  H05K2201/0344 ,  H05K2203/0315 ,  H05K2203/0793 ,  H05K2203/095 ,  Y10T29/49126 ,  H01L2224/0401

摘要： Provided is a wiring board and production method thereof, wherein production of wiring by a full additive method is achieved. This is extremely useful in forming fine copper wiring featuring a high adhesion on an insulating resin substrate. A resin having an excellent alkali resistance is used as the insulating resin substrate, and the copper wiring is formed on the insulating resin substrate through a degenerated layer containing amide group and a metallic oxide layer of a metal having a reduction potential more base than that of copper. The degenerated layer can be provided by, e.g., introduction of amide group into the surface of the insulating resin substrate. The copper can be formed by processes including electroless plating. The insulating resin substrate has superb heat resistance and dimensional stability, and the formed structure can provide a highly packed wiring board.

摘要翻译： 本发明提供一种布线基板及其制造方法，其特征在于，通过全加法制造布线。 这对于在绝缘树脂基板上形成具有高粘附性的细铜布线非常有用。 使用耐碱性优异的树脂作为绝缘树脂基板，并且通过包含酰胺基的退化层和金属氧化物层，在绝缘树脂基板上形成铜布线，该金属氧化物层的还原电位比 铜。 退化层可以通过例如将酰胺基引入绝缘树脂基材的表面来提供。 铜可以通过包括无电镀的工艺形成。 绝缘树脂基板具有极好的耐热性和尺寸稳定性，并且所形成的结构可以提供高度封装的布线板。

公开(公告)号：US09040251B2

公开(公告)日：2015-05-26

申请号：US13388671

申请日：2010-07-29

申请人： Yasuhiko Tada ,  Hiroshi Yoshida ,  Toshiro Saito ,  Masatoshi Narahara ,  Hiroaki Nakagawa

发明人： Yasuhiko Tada ,  Hiroshi Yoshida ,  Toshiro Saito ,  Masatoshi Narahara ,  Hiroaki Nakagawa

IPC分类号： G01N33/53 ,  C07K1/04 ,  G01N33/551 ,  G01N33/543 ,  A61K39/00

CPC分类号： G01N33/54353 ,  A61K2039/625 ,  G01N33/543 ,  G01N33/54393

摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.

摘要翻译： 本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。 生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。