公开(公告)号：US20080207459A1

公开(公告)日：2008-08-28

申请号：US12026412

申请日：2008-02-05

申请人： Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Phillip A. Patten

发明人： Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Phillip A. Patten

IPC分类号： C40B10/00 ,  C40B50/00 ,  C40B30/00 ,  C40B30/02 ,  C40B30/04 ,  C07K1/00 ,  C40B40/08 ,  C12N5/06 ,  C12N15/00

CPC分类号： C07K16/1271 ,  C07K16/00 ,  C07K16/1275 ,  C07K2317/24

摘要： Methods for improving antibodies by a variety of DNA diversification and selection procedures are provided. Improvements include increases in affinity, alterations in specificity and effector function, as well as reduced antigenicity, e.g. humanization. Libraries of recombinant antibody sequences are provided, as are cells expressing members of such libraries. Novel phage display vectors are provided. Methods for the coevolution of an antibody and its cognate antigen are provided. Coevolution is used to evolve HIV envelope proteins with increased antigenicity and broadly neutralizing antibodies that interact therewith. Methods of improving antibodies for use in the detection of biological warfare agents are provided.

摘要翻译： 提供了通过多种DNA多样化和选择程序改进抗体的方法。 改善包括亲和力的增加，特异性和效应子功能的改变，以及降低的抗原性，例如。 人性化。 提供重组抗体序列的文库，以及表达这些文库成员的细胞。 提供了新的噬菌体展示载体。 提供了抗体及其同源抗原共转化方法。 共转化用于发展具有增加的抗原性的HIV包膜蛋白和与之相互作用的广泛中和抗体。 提供了改进用于检测生物战剂的抗体的方法。

公开(公告)号：US06287862B1

公开(公告)日：2001-09-11

申请号：US09626410

申请日：2000-07-26

申请人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steven H. Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steven H. Bass

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US07794718B2

公开(公告)日：2010-09-14

申请号：US12251960

申请日：2008-10-15

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

IPC分类号： A61K39/00 ,  C07K14/44 ,  C07K14/705 ,  C12P21/08

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.

摘要翻译： 本发明提供免疫抑制多肽和编码这种多肽的核酸。 一方面，本发明提供突变CTLA-4多肽和编码突变CTLA-4多肽的核酸。 还提供了用于利用这些多肽和核酸的组合物和方法。

公开(公告)号：US5688666A

公开(公告)日：1997-11-18

申请号：US182530

申请日：1994-01-14

申请人： Steven H. Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells

发明人： Steven H. Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells

IPC分类号： C07K14/575 ,  C07K14/61 ,  C12N15/10 ,  C12N15/62 ,  C40B40/02 ,  C12P21/06

CPC分类号： C40B40/02 ,  C07K14/575 ,  C07K14/61 ,  C12N15/1037 ,  C12N15/62 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  G01N2333/61

摘要： A method for selecting novel proteins such as growth hormone variants having altered binding properties for a growth hormone receptor molecule is provided. The method comprises fusing a gene encoding a hormone to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disposed are preferred phagemid expression vectors and selected human growth hormone variants.

摘要翻译： 提供了一种用于选择具有改变的生长激素受体分子结合特性的生长激素变体的新蛋白质的方法。 该方法包括将编码激素的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 优选的噬菌粒表达载体和选择的人生长激素变体。

公开(公告)号：US20110166324A1

公开(公告)日：2011-07-07

申请号：US13011497

申请日：2011-01-21

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

IPC分类号： C07K14/00

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.

公开(公告)号：US20110129875A1

公开(公告)日：2011-06-02

申请号：US13011042

申请日：2011-01-21

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.

摘要翻译： 本发明提供免疫抑制多肽和编码这种多肽的核酸。 一方面，本发明提供突变CTLA-4多肽和编码突变CTLA-4多肽的核酸。 还提供了用于利用这些多肽和核酸的组合物和方法。

公开(公告)号：US20090258031A1

公开(公告)日：2009-10-15

申请号：US12251960

申请日：2008-10-15

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin ,  Sridhar Viswanathan ,  Brent R. Larsen

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin ,  Sridhar Viswanathan ,  Brent R. Larsen

IPC分类号： A61K39/00 ,  C07K14/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/00 ,  A61K38/16 ,  C12N5/02 ,  C12P21/06

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.

摘要翻译： 本发明提供免疫抑制多肽和编码这种多肽的核酸。 一方面，本发明提供突变CTLA-4多肽和编码突变CTLA-4多肽的核酸。 还提供了用于利用这些多肽和核酸的组合物和方法。

公开(公告)号：US08283447B2

公开(公告)日：2012-10-09

申请号：US13011463

申请日：2011-01-21

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

IPC分类号： C07K14/705

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.

公开(公告)号：US08252727B2

公开(公告)日：2012-08-28

申请号：US12026412

申请日：2008-02-05

申请人： Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Philip A. Patten

发明人： Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Philip A. Patten

IPC分类号： C07K1/00 ,  C12N15/00 ,  C40B30/00 ,  C40B30/02 ,  C40B30/04 ,  C40B40/08 ,  C40B50/00

CPC分类号： C07K16/1271 ,  C07K16/00 ,  C07K16/1275 ,  C07K2317/24

摘要： Methods for improving antibodies by a variety of DNA diversification and selection procedures are provided. Improvements include increases in affinity, alterations in specificity and effector function, as well as reduced antigenicity, e.g. humanization. Libraries of recombinant antibody sequences are provided, as are cells expressing members of such libraries. Novel phage display vectors are provided. Methods for the coevolution of an antibody and its cognate antigen are provided. Coevolution is used to evolve HIV envelope proteins with increased antigenicity and broadly neutralizing antibodies that interact therewith. Methods of improving antibodies for use in the detection of biological warfare agents are provided.

摘要翻译： 提供了通过多种DNA多样化和选择程序改进抗体的方法。 改善包括亲和力的增加，特异性和效应子功能的改变，以及降低的抗原性，例如。 人性化。 提供重组抗体序列的文库，以及表达这些文库成员的细胞。 提供了新的噬菌体展示载体。 提供了抗体及其同源抗原共转化方法。 共转化用于发展具有增加的抗原性的HIV包膜蛋白和与之相互作用的广泛中和抗体。 提供了改进用于检测生物战剂的抗体的方法。

公开(公告)号：US20110130546A1

公开(公告)日：2011-06-02

申请号：US13011463

申请日：2011-01-21

申请人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

发明人： Erik E. Karrer ,  Madan M. Paidhungat ,  Steven H. Bass ,  Margaret Neighbors ,  Juha Punnonen ,  Steven J. Chapin

IPC分类号： C07K16/18

CPC分类号： C07K14/70521 ,  C07K2319/30

摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.