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公开(公告)号：US5688666A
公开(公告)日：1997-11-18
申请号：US182530
申请日：1994-01-14
申请人： Steven H. Bass , Ronald Greene , Henry B. Lowman , James A. Wells
发明人： Steven H. Bass , Ronald Greene , Henry B. Lowman , James A. Wells
CPC分类号： C40B40/02 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , G01N2333/61
摘要： A method for selecting novel proteins such as growth hormone variants having altered binding properties for a growth hormone receptor molecule is provided. The method comprises fusing a gene encoding a hormone to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disposed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了一种用于选择具有改变的生长激素受体分子结合特性的生长激素变体的新蛋白质的方法。 该方法包括将编码激素的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 优选的噬菌粒表达载体和选择的人生长激素变体。
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2.发明申请公开(公告)号：US20120252681A1
公开(公告)日：2012-10-04
申请号：US13310332
申请日：2011-12-02
申请人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C40B10/00
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。
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公开(公告)号：US08029988B2
公开(公告)日：2011-10-04
申请号：US11987555
申请日：2007-11-30
申请人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
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公开(公告)号：US07629170B2
公开(公告)日：2009-12-08
申请号：US11506215
申请日：2006-08-17
申请人： Stephen delCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen delCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
CPC分类号： C12R1/465 , A01K2267/02 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/90 , C12P17/162 , C12Q1/6811 , C12Q1/6827
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
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公开(公告)号：US06287862B1
公开(公告)日：2001-09-11
申请号：US09626410
申请日：2000-07-26
申请人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda Castle , Claus M. Krebber , Steven H. Bass
发明人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda Castle , Claus M. Krebber , Steven H. Bass
IPC分类号： C12N1500
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
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公开(公告)号：US08377681B2
公开(公告)日：2013-02-19
申请号：US13310332
申请日：2011-12-02
申请人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
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公开(公告)号：US07794718B2
公开(公告)日：2010-09-14
申请号：US12251960
申请日：2008-10-15
申请人： Erik E. Karrer , Madan M. Paidhungat , Steven H. Bass , Margaret Neighbors , Juha Punnonen , Steven J. Chapin
发明人： Erik E. Karrer , Madan M. Paidhungat , Steven H. Bass , Margaret Neighbors , Juha Punnonen , Steven J. Chapin
IPC分类号： A61K39/00 , C07K14/44 , C07K14/705 , C12P21/08
CPC分类号： C07K14/70521 , C07K2319/30
摘要： The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided.
摘要翻译： 本发明提供免疫抑制多肽和编码这种多肽的核酸。 一方面，本发明提供突变CTLA-4多肽和编码突变CTLA-4多肽的核酸。 还提供了用于利用这些多肽和核酸的组合物和方法。
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公开(公告)号：US20100184627A1
公开(公告)日：2010-07-22
申请号：US12557829
申请日：2009-09-11
申请人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C40B50/00
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译： 提供重组核酸的方法，包括同源核酸。 还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。
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公开(公告)号：US07619067B2
公开(公告)日：2009-11-17
申请号：US11383798
申请日：2006-05-17
申请人： Madan M. Paidhungat , Ian J. Sas , Thomas Bouquin , David Lin , Teddy Chen , Douglas Guptill , Amy Brideau-Andersen , Steven H. Bass , Phillip A. Patten
发明人： Madan M. Paidhungat , Ian J. Sas , Thomas Bouquin , David Lin , Teddy Chen , Douglas Guptill , Amy Brideau-Andersen , Steven H. Bass , Phillip A. Patten
摘要： The present invention provides evolved interferon-alpha polypeptides, and conjugates thereof, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
摘要翻译： 本发明提供了进化型干扰素-α多肽及其缀合物，以及编码多肽的核酸。 本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。
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公开(公告)号：US06426224B1
公开(公告)日：2002-07-30
申请号：US09626528
申请日：2000-07-27
申请人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C12N1500
CPC分类号： C12N15/11 , A61K39/00 , B01J2219/00689 , C07K14/005 , C07K14/505 , C07K14/535 , C12N9/16 , C12N15/1031 , C12N15/1034 , C12N2740/16122
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译： 提供重组核酸的方法，包括同源核酸。 还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。
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