公开(公告)号：US07232893B2

公开(公告)日：2007-06-19

申请号：US10888962

申请日：2004-07-09

Applicant: Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

Inventor： Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

IPC: C07K1/18 ,  C12N5/00

CPC classification number: C07K14/4747

Abstract: The present invention provides compositions and methods for selectively inhibiting the proliferation of stellate cells, which are important for the development of liver fibrosis upon liver injury. The invention describes conditioned media from immortalized hepatocytes as containing a death factor that induces apoptosis of activated liver stellate cells. This pro-apoptotic activity is shown to be associated with an 80 kDa protein, which is associated with a fetuin peptide sequence and an albumin peptide sequence.

Abstract translation: 本发明提供了选择性抑制星状细胞增殖的组合物和方法，其对于肝损伤时肝纤维化的发展是重要的。 本发明描述了来自永生化肝细胞的条件培养基，其含有诱导活化的肝星状细胞凋亡的死亡因子。 该促凋亡活性显示与80kDa蛋白质相关，其与胎球蛋白肽序列和白蛋白肽序列相关。

公开(公告)号：US20130331456A1

公开(公告)日：2013-12-12

申请号：US13821682

申请日：2011-09-13

Applicant: Arnab Basu ,  Debra M. Mills ,  John D. Williams ,  Bing Li ,  Norton P. Peet ,  Terry L. Bowlin

Inventor： Arnab Basu ,  Debra M. Mills ,  John D. Williams ,  Bing Li ,  Norton P. Peet ,  Terry L. Bowlin

IPC: C07C311/44

CPC classification number: C07C311/44 ,  A61K31/18

Abstract: The present invention is related to the development of compounds and methods for inhibiting viral infection in a mammal. A pseudotype virus was developed for use in a high throughput assay for identifying nonpeptidic small molecule inhibitors that prevent viral entry into a host cell.

Abstract translation: 本发明涉及抑制哺乳动物中病毒感染的化合物和方法的开发。 开发了一种假型病毒用于高通量测定，用于鉴定预防病毒进入宿主细胞的非肽小分子抑制剂。

公开(公告)号：US20080081044A1

公开(公告)日：2008-04-03

申请号：US11506161

申请日：2006-08-17

Applicant: Ratna Ray ,  Ranjit Ray ,  Arnab Basu ,  Tatsuo Kanda

Inventor： Ratna Ray ,  Ranjit Ray ,  Arnab Basu ,  Tatsuo Kanda

IPC: A61K39/29 ,  C12N5/08 ,  C12N15/86

CPC classification number: C12N7/00 ,  A61K2039/525 ,  C12N2770/24251 ,  C12Q1/18 ,  G01N33/5067 ,  G01N2333/18

Abstract: The present invention provides a cell line capable producing infectious hepatitis C virus 1a (HCV 1a) particles in culture. Disclosed are compositions and methods for an HCV 1a (clone H77) transfected immortal human hepatocyte (IHH) capable of generating infectious HCV 1a virus particles in culture. Also disclosed are methods of using the cell line, or HCV 1a virus particles derived from said cell line, to screen for potential therapeutic agents which interfere with HCV 1a virus propagation to treat hepatic disease.

Abstract translation: 本发明提供了一种在培养中能够产生感染性丙型肝炎病毒1a（HCV1a）颗粒的细胞系。 公开了能够在培养中产生感染性HCV 1a病毒颗粒的HCV 1a（克隆H77）转染的永生人肝细胞（IHH）的组合物和方法。 还公开了使用细胞系或源自所述细胞系的HCV 1a病毒颗粒筛选干扰HCV 1a病毒繁殖以治疗肝脏疾病的潜在治疗剂的方法。

公开(公告)号：US20060275838A1

公开(公告)日：2006-12-07

申请号：US11436384

申请日：2006-05-18

Applicant: Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

Inventor： Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

IPC: G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C07K14/475 ,  C12N9/99

CPC classification number: C07K14/4747 ,  C07K14/4703 ,  C07K14/4716 ,  C12P21/06 ,  G01N2800/085

Abstract: The present invention provides compositions and methods for selectively inhibiting the proliferation of stellate cells, which are important for the development of liver fibrosis upon liver injury. The invention describes conditioned media from immortalized hepatocytes as containing a death factor that induces apoptosis of activated liver stellate cells. This pro-apoptotic activity is shown to be associated with the peptide sequence of the actin depolymerizing molecule gelsolin and or fragments thereof. The apoptotic activity is increased upon incubation of immunoglobulins with the stellate death factor.

Abstract translation: 本发明提供了选择性抑制星状细胞增殖的组合物和方法，其对于肝损伤时肝纤维化的发展是重要的。 本发明描述了来自永生化肝细胞的条件培养基，其含有诱导活化的肝星状细胞凋亡的死亡因子。 该促凋亡活性显示与肌动蛋白解聚分子凝溶胶蛋白及其片段的肽序列相关。 免疫球蛋白与星状死亡因子的孵育时，凋亡活性增加。

公开(公告)号：US09212134B2

公开(公告)日：2015-12-15

申请号：US13821682

申请日：2011-09-13

Applicant: Arnab Basu ,  Debra M. Mills ,  John D. Williams ,  Bing Li ,  Norton P. Peet ,  Terry L. Bowlin

Inventor： Arnab Basu ,  Debra M. Mills ,  John D. Williams ,  Bing Li ,  Norton P. Peet ,  Terry L. Bowlin

IPC: C07C311/44 ,  C07C311/32 ,  A61K31/18 ,  A61P31/16

CPC classification number: C07C311/44 ,  A61K31/18

Abstract: The present invention is related to the development of compounds and methods for inhibiting viral infection in a mammal. A pseudotype virus was developed for use in a high throughput assay for identifying nonpeptidic small molecule inhibitors that prevent viral entry into a host cell.

Abstract translation: 本发明涉及抑制哺乳动物中病毒感染的化合物和方法的开发。 开发了一种假型病毒用于高通量测定，用于鉴定预防病毒进入宿主细胞的非肽小分子抑制剂。

公开(公告)号：US20050266531A1

公开(公告)日：2005-12-01

申请号：US10888962

申请日：2004-07-09

Applicant: Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

Inventor： Ranjit Ray ,  Ratna Ray ,  Arnab Basu ,  Yie-Hwa Chang

IPC: C07H21/04 ,  C07K14/47 ,  C07K14/525 ,  C12N5/08 ,  C12P21/02

CPC classification number: C07K14/4747

Abstract: The present invention provides compositions and methods for selectively inhibiting the proliferation of stellate cells, which are important for the development of liver fibrosis upon liver injury. The invention describes conditioned media from immortalized hepatocytes as containing a death factor that induces apoptosis of activated liver stellate cells. This pro-apoptotic activity is shown to be associated with an 80 kDa protein, which is associated with a fetuin peptide sequence and an albumin peptide sequence.

Abstract translation: 本发明提供了选择性抑制星状细胞增殖的组合物和方法，其对于肝损伤时肝纤维化的发展是重要的。 本发明描述了来自永生化肝细胞的条件培养基，其含有诱导活化的肝星状细胞凋亡的死亡因子。 该促凋亡活性显示与80kDa蛋白质相关，其与胎球蛋白肽序列和白蛋白肽序列相关。

公开(公告)号：US20050130157A1

公开(公告)日：2005-06-16

申请号：US10502250

申请日：2003-01-22

Applicant: Philip Serwer ,  Gary Griess ,  Jonathan Valvano ,  Arnab Basu

Inventor： Philip Serwer ,  Gary Griess ,  Jonathan Valvano ,  Arnab Basu

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44713 ,  G01N27/44769 ,  G01N27/44773

Abstract: Systems and methods for improving the resolution of macromolecules during electrophoresis. A method of creating an electrical field-rectifying fractionation-ratchet includes obtaining a fractionated particle that has an electrophoretic mobility that varies when an electric field varies, applying a pulsed electrical field to the fractionated particle, and varying a plurality of pulses of the electrical field repeatedly. Another method includes preparative gel electrophoresis that utilizes a continuous fractionation method made possible by an electrophoretic ratchet-generating field. Another method includes implementing cyclic electrophoresis including analyzing a sample by constant field electrophoresis; and enhancing the sample by an electrophoretic ratchet. Another method includes error checking during cyclic electrophoresis comprising: analyzing a sample by constant field electrophoresis; checking for errors in the sample; and enhancing the sample by an electrophoretic ratchet.

Abstract translation: 用于提高电泳时大分子分辨率的系统和方法。 一种产生电场整流分馏棘轮的方法包括获得具有电场迁移率的分级分离颗粒，其在电场变化时变化，向分级颗粒施加脉冲电场，并改变电场的多个脉冲 反复。 另一种方法包括制备性凝胶电泳，其利用通过电泳棘轮产生场可能的连续分级方法。 另一种方法包括实施循环电泳，包括通过恒定电场电泳分析样品; 并通过电泳棘轮增强样品。 另一种方法包括循环电泳期间的错误检查，包括：通过恒定电场电泳分析样品; 检查样品中的错误; 并通过电泳棘轮增强样品。

公开(公告)号：US09101635B2

公开(公告)日：2015-08-11

申请号：US13383345

申请日：2010-07-10

Applicant: Arnab Basu ,  Debra M. Mills ,  Norton P. Peet ,  John D. Williams

Inventor： Arnab Basu ,  Debra M. Mills ,  Norton P. Peet ,  John D. Williams

IPC: A61K31/18 ,  A61K31/5513 ,  C07D213/74 ,  C07D215/40 ,  C07D243/12 ,  C07D249/10 ,  C07D307/71 ,  C07D405/06 ,  C07D405/12 ,  C07D405/14 ,  C07D409/04 ,  C07D471/04 ,  C07D491/22

CPC classification number: A61K31/5513 ,  A61K31/18 ,  C07D213/74 ,  C07D215/40 ,  C07D243/12 ,  C07D249/10 ,  C07D307/71 ,  C07D405/06 ,  C07D405/12 ,  C07D405/14 ,  C07D409/04 ,  C07D471/04 ,  C07D491/22 ,  A61K2300/00

Abstract: Organic compounds showing the ability to inhibit viral glycoprotein (GP)-mediated entry of a filovirus into a host cell are disclosed. The disclosed filovirus entry inhibitor compounds are useful for treating, preventing, or reducing the spread of infections by filovirus including the type species Marburg virus (MARV) and Ebola virus (EBOV). Preferred inhibitors of the invention provide therapeutic agents for combating the Ivory Coast, Sudan, Zaire, Bundibugyo, and Reston Ebola virus strains.

Abstract translation: 公开了显示抑制病毒糖蛋白（GP）介导的病毒入宿主细胞的能力的有机化合物。 所公开的叶病毒入侵抑制剂化合物可用于治疗，预防或减少包括类型物种马尔堡病毒（MARV）和埃博拉病毒（EBOV）的病毒感染的扩散。 本发明优选的抑制剂提供用于对抗科特迪瓦海岸，苏丹，扎伊尔，Bundibugyo和Reston埃博拉病毒毒株的治疗剂。