公开(公告)号：US20170166937A1

公开(公告)日：2017-06-15

申请号：US15116896

申请日：2015-05-02

申请人： BASF SE

发明人： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  C07K14/285 ,  C12N1/20 ,  C12N15/52

摘要： The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

公开(公告)号：US20140242704A1

公开(公告)日：2014-08-28

申请号：US14272781

申请日：2014-05-08

申请人： BASF SE

发明人： Oskar Zelder ,  Corinna Klopprogge ,  Burkhard Kröger ,  Hartwig Schröder ,  Stefan Haefner

IPC分类号： C12N15/63

CPC分类号： C12N15/63 ,  C07K14/34 ,  C12N15/77 ,  C12P13/04

摘要： The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.

摘要翻译： 本发明涉及核酸序列用于调节基因的转录和表达，新型启动子和表达单元本身的用途，用于改变或引起基因的转录速率和/或表达速率的方法，包含表达单位的表达盒 具有改变或引起的转录速率和/或表达速率的转基因微生物，以及通过培养转基因微生物来制备生物合成产物的方法。

公开(公告)号：US20200181653A1

公开(公告)日：2020-06-11

申请号：US16614772

申请日：2018-05-18

申请人： BASF SE

发明人： Hartwig Schroeder ,  Stefan Haefner ,  Oskar Zelder ,  Christoph Wittmann ,  Anna Christine Schroer ,  Birgit Hoff ,  Nico Boehmer

IPC分类号： C12P7/18 ,  C12N1/20

摘要： Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

公开(公告)号：US10597429B2

公开(公告)日：2020-03-24

申请号：US15779646

申请日：2016-12-02

申请人： BASF SE

发明人： Stefan Haefner ,  Andreas Thywissen ,  Holger Hartmann ,  Nico Boehmer

IPC分类号： C12P21/00 ,  C12P21/02 ,  C12Q1/34 ,  C12N1/14 ,  C12N15/01 ,  C12N15/80 ,  C12P19/14 ,  C07K14/37 ,  C12N9/42

摘要： The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.

公开(公告)号：US10513693B2

公开(公告)日：2019-12-24

申请号：US15127181

申请日：2015-03-18

申请人： BASF SE

发明人： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C07K14/285

摘要： The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).

公开(公告)号：US20180030418A1

公开(公告)日：2018-02-01

申请号：US15127181

申请日：2015-03-18

申请人： BASF SE

发明人： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC分类号： C12N9/04 ,  C12N9/10

CPC分类号： C12N9/0006 ,  C07K14/285 ,  C12N9/1029 ,  C12P7/46 ,  C12Y101/01027 ,  C12Y203/01054

摘要： The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).

公开(公告)号：US20170073665A1

公开(公告)日：2017-03-16

申请号：US15308874

申请日：2015-05-07

申请人： BASF SE

发明人： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schroeder ,  Oskar Zelder

IPC分类号： C12N15/10 ,  C12N15/74 ,  C12P7/46

CPC分类号： C12N15/102 ,  C07K14/195 ,  C12N15/74 ,  C12N2510/02 ,  C12P7/46 ,  C12Y207/11

摘要： The present invention relates to a modified microorganism having, compared to its wildtype,—a reduced activity of an enzyme encoded by the ptsA-gene,—a reduced activity of an enzyme encoded by the ptsH-gene or—a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.

摘要翻译： 本发明涉及一种经修饰的微生物，其与野生型相比具有降低的由ptsA基因编码的酶的活性， - 由ptsH基因编码的酶的活性降低或酶活性降低 由ptsA基因编码并且由ptsH基因编码的酶的活性降低，其中来源于修饰微生物的野生型属于巴氏杆菌科。 本发明还涉及生产琥珀酸的方法和使用改性微生物。

公开(公告)号：US20160362696A1

公开(公告)日：2016-12-15

申请号：US15116600

申请日：2015-02-06

申请人： BASF SE

发明人： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC分类号： C12N15/52 ,  C12N15/74 ,  C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C12N9/12

CPC分类号： C12N15/52 ,  C12N1/02 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01 ,  C12Y203/01054 ,  C12Y207/08031

摘要： The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

摘要翻译： 本发明涉及与其野生型相比具有由wcaJ基因编码的酶的活性降低的修饰微生物。 本发明还涉及有机化合物的制造方法和改性微生物的用途。

公开(公告)号：US20160348082A1

公开(公告)日：2016-12-01

申请号：US15116669

申请日：2015-02-02

申请人： BASF SE

发明人： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oakar Zelder ,  Gregory von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker ,  Anna Lange ,  Benjamin J. Lyons ,  Thomas J. Lyons ,  Eudes De Crecy ,  Ewa Hughes

IPC分类号： C12N9/12 ,  C12P7/46

CPC分类号： C12N9/1205 ,  C12N15/52 ,  C12P7/46 ,  C12Y207/0104

摘要： The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

摘要翻译： 本发明涉及与其野生型相比具有由pykA基因编码的酶的活性降低的修饰微生物。 本发明还涉及一种生产有机化合物的方法和使用改性微生物。

公开(公告)号：US20150197776A1

公开(公告)日：2015-07-16

申请号：US14676317

申请日：2015-04-01

申请人： BASF SE

发明人： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

摘要： The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

摘要翻译： 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。