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公开(公告)号：US09453208B2
公开(公告)日：2016-09-27
申请号：US14543625
申请日：2014-11-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yan Wang
CPC classification number: C12N9/1276 , C07K14/00 , C07K14/001 , C07K19/00 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y207/07049 , Y02P20/52
Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
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公开(公告)号：US20160060616A1
公开(公告)日：2016-03-03
申请号：US14831042
申请日：2015-08-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yan Wang , Peter Vander Horn , Lei Xi
CPC classification number: C12N15/10 , C07K14/195 , C07K2319/00 , C07K2319/80 , C12N9/1252 , C12N15/62 , C12P19/34 , C12Q1/686 , C12Y207/07007
Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
Abstract translation: 本发明提供使用改进的核酸聚合酶的产生来测序和进行聚合酶反应的方法。 改进是以提高聚合酶的持续性的方式将序列非特异性核酸结合结构域与酶的融合。
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公开(公告)号：US20150218536A1
公开(公告)日：2015-08-06
申请号：US14543625
申请日：2014-11-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yan Wang
IPC: C12N9/12
CPC classification number: C12N9/1276 , C07K14/00 , C07K14/001 , C07K19/00 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y207/07049 , Y02P20/52
Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
Abstract translation: 本发明提供了改进的新型核酸修饰酶的产生。 改进是以提高酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。
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公开(公告)号：US08900846B2
公开(公告)日：2014-12-02
申请号：US14058044
申请日：2013-10-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yan Wang
CPC classification number: C12N9/1276 , C07K14/00 , C07K14/001 , C07K19/00 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y207/07049 , Y02P20/52
Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
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公开(公告)号：US20130203122A1
公开(公告)日：2013-08-08
申请号：US13681326
申请日：2012-11-19
Applicant: Bio Rad Laboratories, Inc.
Inventor： Xiao-Song Gong , Yan Wang
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C07K14/195 , C12Q1/6844 , C12Q2527/127 , C12Q2527/125 , C12Q2521/307
Abstract: The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule.
Abstract translation: 本发明提供了扩增核酸分子的方法。 该方法包括将RNA模板与具有逆转录酶，DNA聚合酶和RT抑制减少剂的组合物混合。 RT抑制减少剂可以是Sso7d，Sac7d，Sac7e，Sso7e，AluI甲基化酶，苏拉明，硫代磷酸寡脱氧胞嘧啶，硫代磷酸寡脱氧腺嘌呤，硫代磷酸酯寡脱氧胸苷或聚（rA）（dT）。 混合形成混合物，其在足以合成与所有或部分RNA模板互补的DNA分子的条件下孵育，从而扩增核酸分子。
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Patent Agency Ranking
公开(公告)号：US11208635B2
公开(公告)日：2021-12-28
申请号：US16788660
申请日：2020-02-12
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Peter B. Vander Horn , Yan Wang
IPC: C12N9/12
Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.
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公开(公告)号：US10988762B2
公开(公告)日：2021-04-27
申请号：US15895504
申请日：2018-02-13
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Xiao-Song Gong , Yan Wang
IPC: C12N9/10 , C12N9/22 , C12N15/10 , C12Q1/686 , C12Q1/6844
Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.
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公开(公告)号：US10351831B2
公开(公告)日：2019-07-16
申请号：US15801114
申请日：2017-11-01
Applicant: Bio-Rad Laboratories, Inc.
Abstract: Fusion polypeptides having a heterologous 5′-3′ exonuclease domain linked to a polymerase that does not naturally have 5′-3′ exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.
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公开(公告)号：US10072285B2
公开(公告)日：2018-09-11
申请号：US14828242
申请日：2015-08-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng , Xiao-Song Gong , Yan Wang , Adam M. McCoy
IPC: C12Q1/68 , C12Q1/6806 , C12P19/34 , C12Q1/686
CPC classification number: C12Q1/6806 , C07K2319/80 , C12N15/1096 , C12P19/34 , C12Q1/6853 , C12Q1/686 , C12Q2521/107 , C12Q2521/131 , C12Q2521/327 , C12Q2525/207 , C12Q2521/101 , C12Q2522/101
Abstract: Methods of detecting and amplifying short RNAs are provided.
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公开(公告)号：US09840697B2
公开(公告)日：2017-12-12
申请号：US14562396
申请日：2014-12-05
Applicant: Bio-Rad Laboratories, Inc.
CPC classification number: C12N9/1252 , C07K2319/80 , C12N9/22 , C12Q1/686
Abstract: Fusion polypeptides having a heterologous 5′-3′ exonuclease domain linked to a polymerase that does not naturally have 5′-3′ exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.
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