公开(公告)号：US20250020574A1

公开(公告)日：2025-01-16

申请号：US18887450

申请日：2024-09-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11814671B2

公开(公告)日：2023-11-14

申请号：US17170576

申请日：2021-02-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6811 ,  C12N15/1065 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2600/178

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US20230056356A1

公开(公告)日：2023-02-23

申请号：US18048512

申请日：2022-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US11504714B2

公开(公告)日：2022-11-22

申请号：US16790583

申请日：2020-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US11275015B2

公开(公告)日：2022-03-15

申请号：US16732556

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10 ,  B01L3/02 ,  G06K9/20 ,  G06K9/00 ,  C12M1/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20210164023A1

公开(公告)日：2021-06-03

申请号：US17170576

申请日：2021-02-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US10947581B2

公开(公告)日：2021-03-16

申请号：US16821644

申请日：2020-03-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US10914672B2

公开(公告)日：2021-02-09

申请号：US17025326

申请日：2020-09-18

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/10 ,  G01N15/14 ,  G01N1/20 ,  B01L3/00 ,  C12M1/00 ,  G01N1/28 ,  G01N1/40 ,  G06K9/20 ,  G01N35/10 ,  G06K9/00 ,  G01N35/00 ,  B01L3/02 ,  G01N15/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.