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    Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide
    21.
    发明授权
    Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide 失效
    预功能化核苷酸和使用预功能化核苷酸扩增序列的方法

    公开(公告)号：US06537783B1

    公开(公告)日：2003-03-25

    申请号：US09214658

    申请日：1999-01-11

    申请人： Françoise Guillou-Bonnici ,  Eric Defranc ,  Antoine Hoang ,  Ali Laayoun ,  Jean Lhomme ,  Emmanuelle Trevisiol

    发明人： Françoise Guillou-Bonnici ,  Eric Defranc ,  Antoine Hoang ,  Ali Laayoun ,  Jean Lhomme ,  Emmanuelle Trevisiol

    IPC分类号： C12P1934

    CPC分类号： C12P19/34 ,  C07H19/06 ,  C07H21/00 ,  C12N15/10 ,  C12P19/30 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2525/101

    摘要： A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.

    摘要翻译： 一种靶核酸序列扩增方法，其中至少提供所述目标核酸序列，至少一种对靶序列特异性的寡核苷酸引物，一种或多种酶活性和核苷酸，并且所述靶序列在适合于所述 酶或活性或活性。 根据该方法，至少一个核苷酸是与其它核苷酸不同的前功能化核苷酸，至少在至少一个所述核苷酸的碱基的一个预定位点中包含至少一个未保护的反应性共价功能，以便 得到包括至少一种这样的前官能化核苷酸的前功能化扩增产物。 还公开了用于实施该方法的核苷酸。

    METHOD FOR AMPLIFYING AND/OR DETECTING NUCLEIC ACIDS, KITS AND USES OF SAID METHOD
    22.
    发明申请
    METHOD FOR AMPLIFYING AND/OR DETECTING NUCLEIC ACIDS, KITS AND USES OF SAID METHOD 有权
    用于放大和/或检测核酸的方法，用途和使用方法

    公开(公告)号：US20110294129A1

    公开(公告)日：2011-12-01

    申请号：US13131174

    申请日：2010-01-04

    申请人： Ali Laayoun ,  Alain Troesch

    发明人： Ali Laayoun ,  Alain Troesch

    IPC分类号： C12P19/34 ,  C12N9/12 ,  C12Q1/68

    CPC分类号： C12Q1/6848 ,  C12Q2523/125

    摘要： A method of amplification and/or of detection for removing contaminants in a liquid biological sample containing nucleic acids of interest to amplify, which includes treating the biological sample chemically or enzymatically to convert one type of base of the nucleic acids of interest to another type of base; adding amplification primers, intended for specifically amplifying said converted nucleic acids of interest, each primer being constituted of three different types of bases; adding to the biological sample, after these treatments, amplification reagents the primers previously synthesized; and placing the solution and the reagents in conditions permitting amplification and/or detection of the converted nucleic acids.

    摘要翻译： 一种用于去除含有目的核酸的液体生物样品中的污染物的扩增和/或检测方法，用于扩增，其包括以化学或酶学方式处理生物样品以将目的核酸的一种碱基转化为另一种类型的 基础; 加入用于特异性扩增所述转化的目的核酸的扩增引物，每个引物由三种不同类型的碱基组成; 加入生物样品后，经过这些处理，扩增试剂是先前合成的引物; 并将溶液和试剂置于允许扩增和/或检测转化的核酸的条件下。

    Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
    25.
    发明授权
    Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby 有权
    用于标记核糖核酸的方法和由此获得的标记的RNA片段

    公开(公告)号：US06489114B2

    公开(公告)日：2002-12-03

    申请号：US09737761

    申请日：2000-12-18

    申请人： Ali Laayoun ,  Duc Do ,  Charles G. Miyada

    发明人： Ali Laayoun ,  Duc Do ,  Charles G. Miyada

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C12Q1/6806 ,  C12Q2563/131

    摘要： A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.

    摘要翻译： 提供了用信号扩增标记核糖核酸（RNA）的方法，其包括将RNA片段化以形成RNA片段，将第一配体固定在位于每个的3'末端和5'末端中的至少一个末端磷酸酯 多个RNA片段，末端磷酸酯在片段化期间被释放，并且将多个标记试剂与多个片段中的每一个上的第一配体结合。

    Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules
    26.
    发明授权
    Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules 失效
    带有重氮和硝基功能的标记试剂，合成这些试剂的方法和检测生物分子的方法

    公开(公告)号：US08309695B2

    公开(公告)日：2012-11-13

    申请号：US12451502

    申请日：2008-06-09

    申请人： Alain Laurent ,  Ali Laayoun ,  Mitsuharu Kotera

    发明人： Alain Laurent ,  Ali Laayoun ,  Mitsuharu Kotera

    IPC分类号： C07C245/14 ,  C12Q1/68

    CPC分类号： C07D495/04 ,  Y10T436/13

    摘要： The present invention relates to a labeling reagent of formula: in which: R1 represents at least one detectable label, L and A are each a linker arm, n is an integer equal to 1, and u is an integer between 0 and 2. The present invention also describes a method of synthesizing said markers and also applications for the labeling of biological molecules, more particularly nucleic acids, with a labeling reagent bearing diazo and nitro functions. The invention is particularly suitable for use in the diagnostics field.

    摘要翻译： 本发明涉及下式的标记试剂：其中：R1表示至少一个可检测标记，L和A各自为连接臂，n为等于1的整数，u为0至2之间的整数。 本发明还描述了一种合成所述标记的方法，以及用于用具有重氮和硝基功能的标记试剂标记生物分子，更特别是核酸的应用。 本发明特别适用于诊断领域。

    Process for labeling a ribonucleic acid
    27.
    发明授权
    Process for labeling a ribonucleic acid 失效
    用于标记核糖核酸的方法

    公开(公告)号：US06921640B2

    公开(公告)日：2005-07-26

    申请号：US10101362

    申请日：2002-03-20

    申请人： Ali Laayoun

    发明人： Ali Laayoun

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/02 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/63

    CPC分类号： C07H21/00

    摘要： The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3′ end and/or the 5′ end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.

    摘要翻译： 本发明涉及用于标记合成或天然核糖核酸（RNA）的方法。 它还涉及RNA片段，其已经通过片段化RNA来标记以释放每个片段的末端磷酸酯用于进一步的反应，并且在位于3'末端和/或5'端的释放的磷酸末端标记每个片段， RNA的每个片段的末端，以及例如在医学诊断领域中使用这样的RNA片段。