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公开(公告)号：USD705444S1
公开(公告)日：2014-05-20
申请号：US29403000
申请日：2011-09-30
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公开(公告)号：US20100059440A1
公开(公告)日：2010-03-11
申请号：US12523323
申请日：2008-02-07
申请人： Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
发明人： Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
CPC分类号： B01D71/10 , B01D15/36 , B01D15/3804 , B01D67/0093 , B01D71/16 , B01D2323/30 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28085 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3244
摘要： The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.
摘要翻译： 本发明涉及一种制备能够结合靶分子的多孔交联带电纤维素聚合物膜的方法。 本发明提供了在包含使用可通过本发明的方法获得的膜的溶液中从目标分子与其它组分分离的方法。 该方法在从细胞裂解物和培养物中分离蛋白质和核酸方面具有特殊的用途。
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公开(公告)号：US09522365B2
公开(公告)日：2016-12-20
申请号：US12523323
申请日：2008-02-07
申请人： Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
发明人： Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
IPC分类号： B01D71/16 , B01J20/26 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , B01D71/10 , B01D67/00 , B01D61/14 , B01D15/36 , B01D15/38
CPC分类号： B01D71/10 , B01D15/36 , B01D15/3804 , B01D67/0093 , B01D71/16 , B01D2323/30 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28085 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3244
摘要： The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.
摘要翻译： 本发明涉及一种制备能够结合靶分子的多孔交联带电纤维素聚合物膜的方法。 本发明提供了在包含使用可通过本发明的方法获得的膜的溶液中从目标分子与其它组分分离的方法。 该方法在从细胞裂解物和培养物中分离蛋白质和核酸方面具有特殊的用途。
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24.发明授权Separation media for biomolecules comprising porous hybrid particles containing inorganic metal oxide nanoparticles 有权用于生物分子的分离介质包括含有无机金属氧化物纳米颗粒的多孔混合颗粒公开(公告)号：US08632883B2
公开(公告)日：2014-01-21
申请号：US12864070
申请日：2009-02-12
申请人： Anders Larsson , Ulrika Meyer , Nils Norrman , Johan Ohman
发明人： Anders Larsson , Ulrika Meyer , Nils Norrman , Johan Ohman
CPC分类号： B01J20/28026 , B01D15/34 , B01J20/06 , B01J20/223 , B01J20/28009 , B01J20/282 , C07K1/22
摘要： The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules.
摘要翻译： 本发明涉及分离生物分子。 更详细地说，本发明涉及一种生产包含无机和有机材料的混合颗粒的分离介质的方法以及通过该方法制备的杂化颗粒。 最后，本发明涉及杂交颗粒用于分离生物分子，优选磷蛋白的用途。 该方法包括以下步骤：将无机金属氧化物颗粒加入到有机溶液中以形成混合物; 并且混合物乳化以形成多孔混合颗粒，其中多孔混合颗粒的密度为1.0-1.5g / ml，并且其中无机颗粒具有使其无机颗粒相互作用的活性表面积最大化的形状和尺寸 与生物分子。
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公开(公告)号：US20100294977A1
公开(公告)日：2010-11-25
申请号：US12864070
申请日：2009-02-12
申请人： Anders Larsson , Ulrika Meyer , Nils Norrman , Johan Ohman
发明人： Anders Larsson , Ulrika Meyer , Nils Norrman , Johan Ohman
CPC分类号： B01J20/28026 , B01D15/34 , B01J20/06 , B01J20/223 , B01J20/28009 , B01J20/282 , C07K1/22
摘要： The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules.
摘要翻译： 本发明涉及分离生物分子。 更详细地说，本发明涉及一种生产包含无机和有机材料的混合颗粒的分离介质的方法以及通过该方法制备的杂化颗粒。 最后，本发明涉及杂交颗粒用于分离生物分子，优选磷蛋白的用途。 该方法包括以下步骤：将无机金属氧化物颗粒加入到有机溶液中以形成混合物; 并且混合物乳化以形成多孔混合颗粒，其中多孔混合颗粒的密度为1.0-1.5g / ml，并且其中无机颗粒具有使其无机颗粒相互作用的活性表面积最大化的形状和尺寸 与生物分子。
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公开(公告)号：US20100035273A1
公开(公告)日：2010-02-11
申请号：US12443072
申请日：2007-09-24
申请人： Andreas Axen , Anders Larsson , Nils Norrman
发明人： Andreas Axen , Anders Larsson , Nils Norrman
IPC分类号： G01N33/53 , B01J19/00 , C12M1/00 , G01N33/566
CPC分类号： B01J19/0093 , B01J2219/00837 , B01J2219/0093 , B01L3/502761 , B01L3/502792 , B01L3/5088 , B01L2200/0647 , B01L2300/089 , B01L2400/043 , G01N33/54326 , G01N33/54333
摘要： The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands.
摘要翻译： 本发明涉及用于小规模反应的方法和装置，例如样品中所需物质的样品制备。 在使用装置的方法中，与功能化磁性颗粒混合的样品在装置上的不同工作站之间磁性传递。 该方法使用疏水性表面，例如陪替氏培养皿，其具有例如位于所述疏水性表面上并具有缓冲液，反应物或配体的琼脂糖珠的亲水斑点。
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公开(公告)号：USD595423S1
公开(公告)日：2009-06-30
申请号：US29329147
申请日：2008-12-10
申请人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder
设计人： Bjorn A. Johansson , Nils Norrman , Henrik Ostlin , Sven Wounder
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公开(公告)号：US20090092837A1
公开(公告)日：2009-04-09
申请号：US12282650
申请日：2007-03-20
申请人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias E. Soderman
发明人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias E. Soderman
CPC分类号： G01N33/5434 , B22F2998/00 , B82Y25/00 , C12N11/08 , C12N11/10 , H01F1/0054 , H01F1/37 , Y10T428/2998 , B22F1/0062
摘要： The present invention relates to magnetic beads in the form of composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and thereafter a hydrophilic porous polymer, preferably dextran. This provides porous biocompatible beads without metal leakage. This construction also allows for simple and convenient handling of cell expansion media by magnetism.
摘要翻译： 本发明涉及具有金属颗粒内核的复合珠形式的磁珠，其中涂覆有惰性合成聚合物，然后涂覆亲水性多孔聚合物，优选葡聚糖。 这提供了多孔的生物相容珠，而没有金属泄漏。 这种结构还允许通过磁性简单和方便地处理细胞膨胀介质。
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公开(公告)号：US20080283792A1
公开(公告)日：2008-11-20
申请号：US12090403
申请日：2006-10-23
申请人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias Soderman
发明人： Andreas Axen , Eva Holmgren , Nils Norrman , Tobias Soderman
IPC分类号： B03C1/01
CPC分类号： G01N33/5434 , B01J20/0229 , B01J20/06 , B01J20/24 , B01J20/26 , B01J20/28009 , B01J20/28026 , B01J20/286 , B01J20/3028 , B01J20/3244 , B01J20/3268 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/3285 , B01J20/3289 , B01J20/3293 , B03C1/01 , G01N33/54326 , H01F1/0054 , H01F1/36
摘要： The present invention relates to a separation medium with various functionalities suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The separation medium comprises magnetic metal particles, preferably coated with an inert synthetic polymer, and pre-functionalised beads. These particles and beads are provided encapsulated in a hydrophilic porous polymer, preferably agarose. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer may be provided with ligands having affinity for selected biomolecules.
摘要翻译： 本发明涉及具有适合于例如蛋白质，细胞和病毒分离以及诊断应用和细胞培养的各种功能的分离介质。 分离介质包括优选涂覆有惰性合成聚合物的磁性金属颗粒和预功能化珠。 提供这些颗粒和珠粒包封在亲水性多孔聚合物中，优选琼脂糖。 珠粒可用于细胞培养或色谱法。 当珠子用于色谱法时，琼脂糖层可以具有对选定的生物分子具有亲和力的配体。
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公开(公告)号：US08758682B2
公开(公告)日：2014-06-24
申请号：US12443072
申请日：2007-09-24
申请人： Andreas Axen , Anders Larsson , Nils Norrman
发明人： Andreas Axen , Anders Larsson , Nils Norrman
IPC分类号： C12Q1/68
CPC分类号： B01J19/0093 , B01J2219/00837 , B01J2219/0093 , B01L3/502761 , B01L3/502792 , B01L3/5088 , B01L2200/0647 , B01L2300/089 , B01L2400/043 , G01N33/54326 , G01N33/54333
摘要： The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands.
摘要翻译： 本发明涉及用于小规模反应的方法和装置，例如样品中所需物质的样品制备。 在使用装置的方法中，与功能化磁性颗粒混合的样品在装置上的不同工作站之间磁性传递。 该方法使用疏水性表面，例如陪替氏培养皿，其具有例如位于所述疏水性表面上并具有缓冲液，反应物或配体的琼脂糖珠的亲水斑点。
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