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43 条记录 (耗时 0.044 秒)

    FATTY ACID AND DERIVATIVES PRODUCTION
    24.
    发明申请
    FATTY ACID AND DERIVATIVES PRODUCTION 审中-公开
    脂肪酸和衍生物生产

    公开(公告)号：US20160138061A1

    公开(公告)日：2016-05-19

    申请号：US14943333

    申请日：2015-11-17

    申请人： Thomas HAAS ,  Markus Poetter ,  Steffen Schaffer

    发明人： Thomas HAAS ,  Markus Poetter ,  Steffen Schaffer

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6409 ,  Y02P20/59

    摘要： At least one fatty acid and/or derivative thereof is produced from a gas containing H2, CO2, and O2 by providing a genetically modified hydrogen oxidizing bacterium in an aqueous medium; and contacting the aqueous medium with the gas containing H2, CO2 and O2 in a weight ratio of 20 to 70 (H2): 10 to 45 (CO2): 5 to 35 (O2); wherein the fatty acid contains at least 5 carbon atoms and wherein the hydrogen oxidizing bacterium is genetically modified relative to the wild type bacterium to increase the expression of enzyme E1 that is capable of catalyzing the conversion of acetyl CoA to acyl ACP via malonyl coA and to increase the expression of enzyme E2 that is capable of catalyzing the conversion of Acyl ACP to the fatty acid.

    摘要翻译： 通过在水性介质中提供遗传修饰的氢氧化细菌，从含有H 2，CO 2和O 2的气体产生至少一种脂肪酸和/或其衍生物; 并使含水介质与含有H 2，CO 2和O 2的气体以20至70（H 2）：10至45（CO 2）：5至35（O 2）的重量比接触; 其中所述脂肪酸含有至少5个碳原子，并且其中所述氢氧化细菌相对于野生型细菌被遗传修饰以增加能够通过丙二酰辅酶A催化乙酰辅酶A转化为酰基ACP的酶E1的表达， 增加能够催化酰基ACP向脂肪酸转化的酶E2的表达。

    RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID
    27.
    发明申请
    RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID 审中-公开
    重组细胞生产2-羟基丁酸

    公开(公告)号：US20110171702A1

    公开(公告)日：2011-07-14

    申请号：US13001204

    申请日：2009-04-28

    申请人： Liv Reinecke ,  Steffen Schaffer ,  Achim Marx ,  Markus Poetter ,  Thomas Haas

    发明人： Liv Reinecke ,  Steffen Schaffer ,  Achim Marx ,  Markus Poetter ,  Thomas Haas

    IPC分类号： C12P7/40 ,  C12N5/10 ,  C12N1/00 ,  C12P7/42 ,  C12P7/62

    CPC分类号： C12P7/625 ,  C12P7/42

    摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.

    摘要翻译： 本发明涉及一种经遗传修饰以能够产生比其野生型更多的2-羟基异丁酸或更多含2-羟基异丁酸单体单元的聚羟基链烷酸酯的细胞，其特征在于2-羟基异丁酸或含有2-羟基异丁酸的聚羟基链烷酸酯， 通过作为中间体的乙酰乙酰辅酶A和作为前体的3-羟基丁酰辅酶A制备羟基异丁酸单体单元。

    PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS
    28.
    发明申请
    PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS 有权
    使用遗传工程微生物菌株生产刺槐根

    公开(公告)号：US20100190219A1

    公开(公告)日：2010-07-29

    申请号：US12300397

    申请日：2007-05-11

    申请人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    发明人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    IPC分类号： C12P13/02 ,  C12N1/00 ,  C12N15/74 ,  C12N9/00 ,  C12N9/02 ,  C12N9/78

    CPC分类号： C12N9/80 ,  A61K8/68 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0071 ,  C12N9/1029

    摘要： The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity.

    摘要翻译： 本发明提供基因工程微生物菌株，特别是遗传工程酵母菌株，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 本发明提供了一种获得基因工程的微生物菌株的方法，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 该方法包括以下步骤：a）增加编码具有神经酰胺合成酶活性的酶的多核苷酸和/或具有神经酰胺酶活性的酶的表达，后者能够优先或甚至具体地水解含有类鞘氨醇碱的神经酰胺根据 式I，和/或b）减少编码具有鞘脂和Dgr.8-去饱和酶活性的酶的多核苷酸的表达和/或具有神经酰胺酶活性的酶，后者能够优先或甚至具体地水解含有植物鞘氨醇的神经酰胺或 二氢鞘氨醇作为鞘氨醇碱，并以所需的生产力分离菌株。