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    Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium
    5.
    发明授权
    Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium 有权
    改进疏水性有机溶液与水性培养基分离的方法

    公开(公告)号：US09249435B2

    公开(公告)日：2016-02-02

    申请号：US13721481

    申请日：2012-12-20

    申请人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

    发明人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

    IPC分类号： C12P13/04 ,  C12P7/64 ,  C12N9/02

    CPC分类号： C12P7/6436 ,  C12N9/001 ,  C12P13/04 ,  C12Y103/99003

    摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

    摘要翻译： 提供了一种从水性培养基中改善疏水性有机溶液分离的方法。 该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。 与野生型活性细胞相比，代谢活性细胞的活性降低，活性降低至少是催化脂肪酸一次反应的一种酶。 本发明进一步提供了一种代谢活性细胞，其与野生型相比具有降低的活性，其酶催化脂肪酸的一种反应的反应，包括选自FadA，FadB，FadD的酶 ，FadL和FadE及其变体。

    PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS
    9.
    发明申请
    PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS 有权
    使用遗传工程微生物菌株生产刺槐根

    公开(公告)号：US20100190219A1

    公开(公告)日：2010-07-29

    申请号：US12300397

    申请日：2007-05-11

    申请人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    发明人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    IPC分类号： C12P13/02 ,  C12N1/00 ,  C12N15/74 ,  C12N9/00 ,  C12N9/02 ,  C12N9/78

    CPC分类号： C12N9/80 ,  A61K8/68 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0071 ,  C12N9/1029

    摘要： The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity.

    摘要翻译： 本发明提供基因工程微生物菌株，特别是遗传工程酵母菌株，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 本发明提供了一种获得基因工程的微生物菌株的方法，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 该方法包括以下步骤：a）增加编码具有神经酰胺合成酶活性的酶的多核苷酸和/或具有神经酰胺酶活性的酶的表达，后者能够优先或甚至具体地水解含有类鞘氨醇碱的神经酰胺根据 式I，和/或b）减少编码具有鞘脂和Dgr.8-去饱和酶活性的酶的多核苷酸的表达和/或具有神经酰胺酶活性的酶，后者能够优先或甚至具体地水解含有植物鞘氨醇的神经酰胺或 二氢鞘氨醇作为鞘氨醇碱，并以所需的生产力分离菌株。