公开(公告)号：US20220003728A1

公开(公告)日：2022-01-06

申请号：US17479342

申请日：2021-09-20

Applicant: Illumina, Inc.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US11143638B2

公开(公告)日：2021-10-12

申请号：US17254466

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20210151129A1

公开(公告)日：2021-05-20

申请号：US17254459

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Craig Ciesla ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Arvin Emadi ,  Merek Siu

IPC: G16B50/30 ,  G06F3/06 ,  G16B30/10 ,  C12Q1/6869

Abstract: A system writes input data to a storage device as machine-written polynucleotides; and reads machine written polynucleotides from the storage device as output data. The storage device includes a flow cell including a plurality of storage wells in which machine written polynucleotides may be stored. The storage device may include a set of electrodes corresponding to the storage wells that allow for selective interactions with wells across the surface of a flow cell. Operation of the storage device may include receiving a read request associated with a particular location in the storage device, creating a copy of a nucleotide sequence located at the particular location in the storage device, transferring the copy of the nucleotide sequence to a read location, and reading the copy of the nucleotide sequence at the read location.

公开(公告)号：US12111262B2

公开(公告)日：2024-10-08

申请号：US17419375

申请日：2020-06-25

Applicant: ILLUMINA, INC.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Fabien Abeille ,  Ali Agah ,  Craig Ciesla ,  Aathavan Karunakaran

IPC: G01N21/64 ,  G01N15/1434 ,  G01N21/05 ,  G02B6/12 ,  G02B6/124 ,  H01L27/148

CPC classification number: G01N21/645 ,  G02B6/124 ,  H01L27/14825 ,  G01N15/1436 ,  G01N2021/058 ,  G01N2021/6482 ,  G01N2021/6484 ,  G02B2006/12107 ,  G02B2006/12138

Abstract: Provided herein include various examples of an apparatus, flow cells that include these examples of the apparatus, and methods of making these examples of the apparatus. The apparatus can include a molding layer over a substrate and covering sides of a light detection device. The molding layer comprises a first region and a second region, which, with the active surface of the light detection device, form a contiguous surface. A waveguide integration layer is between the contiguous surface and a waveguide. The waveguide integration layer comprises optical coupling structures over the first and second regions, to optically couple light waves from a light source to the waveguide. The waveguide utilizes the light waves to excite light sensitive materials in nanowells. A nanostructure layer over the waveguide comprises the nanowells. Each nanowell shares a vertical axis with a location on the active surface of the light detection device.

公开(公告)号：US12062674B2

公开(公告)日：2024-08-13

申请号：US17804023

申请日：2022-05-25

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah

IPC: H01L27/146 ,  H01L23/00

CPC classification number: H01L27/14618 ,  H01L24/05 ,  H01L27/14636 ,  H01L27/14643 ,  H01L27/14689 ,  H01L27/14698

Abstract: Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.

公开(公告)号：US20230075101A1

公开(公告)日：2023-03-09

申请号：US17823786

申请日：2022-08-31

Applicant: ILLUMINA, INC.

Inventor： Arvin Emadi

IPC: H01L27/144 ,  B01L3/00 ,  G01N15/14

Abstract: There is set forth herein a device comprising: a detector surface for supporting biological or chemical samples; an array of doped areas formed in a semiconductor formation, wherein the semiconductor formation receives excitation light and emission light from the detector surface, and wherein doped areas of the array of doped areas define photodiodes; a doped region formed in the semiconductor formation in a receive light path of the excitation light and emission light intermediate the detector surface and a doped area of the array of doped areas; and wherein the doped region is configured to impact a travel direction of electrons generated in the doped region as a result of photon absorption.

公开(公告)号：US20220415731A1

公开(公告)日：2022-12-29

申请号：US17820659

申请日：2022-08-18

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah ,  Tara Bozorg-Grayeli

IPC: H01L23/053 ,  H01L23/31 ,  H01L23/00 ,  H01L23/48 ,  B81B1/00 ,  H01L23/15

Abstract: Provided herein include various examples of an apparatus, a sensor system and examples of a method for manufacturing aspects of an apparatus, a sensor system. The apparatus may include a die. The apparatus may also include a substrate comprising a cavity. The die may be oriented in a portion of the cavity in the substrate, where the orientation defines a first space in the cavity adjacent to a first edge of the upper surface of the die and a second space in the cavity adjacent to the second edge of the upper surface of the die. The apparatus may further include fluidics fan-out regions comprising a first cured material deposited in the first space and the second space, a surface of the fluidics fan-out regions being contiguous with the upper surface of the die.

公开(公告)号：US11453003B2

公开(公告)日：2022-09-27

申请号：US17550063

申请日：2021-12-14

Applicant: ILLUMINA, INC.

Inventor： Ali Agah ,  Aathavan Karunakaran ,  Tarun Khurana ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: B01L3/00 ,  G11C13/02 ,  B01J19/00 ,  C12Q1/6874 ,  C12N15/09

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.

公开(公告)号：US20220283087A1

公开(公告)日：2022-09-08

申请号：US17653281

申请日：2022-03-02

Applicant: Illumina, Inc.

Inventor： Mohsen REZAEI ,  Michael BUREK ,  Arvin Emadi ,  Ludovic VINCENT

IPC: G01N21/64 ,  G01N33/543

Abstract: There is set forth herein, in one example, an apparatus. The apparatus can comprise, for example: a first reaction site and a second reaction site over a single pixel. There is set forth herein, in one example, a method. The method can include, for example: detecting a signal emitted from a first reaction site and a second reaction site; determining the identity of a first analyte of interest in a first reaction site using an amplitude of the detected signal; and determining the identity of a second analyte of interest in a second reaction site using the amplitude of the detected signal.

公开(公告)号：US11387269B2

公开(公告)日：2022-07-12

申请号：US15929760

申请日：2020-05-20

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah

IPC: H01L27/146 ,  H01L23/00

Abstract: Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.