公开(公告)号：US4325909A

公开(公告)日：1982-04-20

申请号：US200143

申请日：1980-10-24

申请人： Wallace H. Coulter ,  Walter M. Mena

发明人： Wallace H. Coulter ,  Walter M. Mena

IPC分类号： G01N35/10 ,  G01N1/14 ,  G01N35/06

CPC分类号： G01N35/1011 ,  G01N2035/1025 ,  G01N2035/1086

摘要： A vertically and horizontally movable arm structure for fluid transfer is provided with a tubular member for linear oscillation of a holder for a fluid probe; the tubular member can additionally form a conduit for lead wire connected to the fluid probe. Sensors for the vertical position of the arm and the conductor leads from the sensors can be separately mounted and routed so that the sensor leads do not kink or interfere with the motion of the arm.

摘要翻译： 用于流体传送的垂直和水平可移动的臂结构设置有用于流体探针的保持器的线性振荡的管状构件; 管状构件可另外形成连接到流体探针的引线的导管。 传感器的臂和导体引线的垂直位置的传感器可以单独安装和布线，以便传感器引线不会扭曲或干扰臂的运动。

公开(公告)号：US3963984A

公开(公告)日：1976-06-15

申请号：US520711

申请日：1974-11-04

申请人： Wallace H. Coulter

发明人： Wallace H. Coulter

IPC分类号： G01N15/12 ,  B08B3/10 ,  G01N27/02

CPC分类号： G01N15/1209

摘要： In both the method and system for clearing the debris from the aperture of a particle study device combinations of pulses of predetermined, waveform amplitude and frequency are developed and coupled through the conductive fluid passing through the particle study device aperture. The pulses cause the fluid in the aperture to vaporize and form a gas. The gas explodes away any debris clogging the aperture as it escapes from the aperture.

公开(公告)号：US3944917A

公开(公告)日：1976-03-16

申请号：US387548

申请日：1973-08-13

申请人： Hogg, Walter R. ,  Gerhard A. Liedholz ,  Wallace H. Coulter

发明人： Hogg, Walter R. ,  Gerhard A. Liedholz ,  Wallace H. Coulter

IPC分类号： G01N15/12 ,  G01N33/49 ,  G01R27/26 ,  G01N27/00 ,  G01N27/42 ,  G01R27/14

CPC分类号： G01N15/1227 ,  G01R27/26

摘要： The electrical sensing circuitry includes a power supply and a signal-detecting circuit which are coupled through the resistance of an electrolyte in a liquid in an aperture of a particle-analyzing device between sensing electrodes positioned on either side of the aperture. The liquid containing an electrolyte and the passage of a liquidborne particle through the aperture causes a change in the resistance of the aperture thereby generating a signal which is detected by the signal-detecting circuit. The parameters of the circuit elements are chosen to provide circuit relationships which render the particle-generated signal independent of the diameter of the aperture. This is achieved by utilizing a power source having a low output impedance and a signal-detecting circuit which has a low input impedance for both D.C. and A.C., namely, at the signal frequencies of the signals sensed. The electrical circuitry also may include a conductivity monitoring circuit which may utilize one or more of the sensing electrodes and/or additional electrodes for monitoring changes in conductivity of the electrolyte and for relating these changes in conductivity to the particle-generated signals sensed by the signal-detecting circuit to alter those signals so as to render them independent of electrolyte resistivity.

摘要翻译： 电感测电路包括电源和信号检测电路，其通过位于孔的任一侧的感测电极之间的粒子分析装置的孔中的液体中的电解液的电阻耦合。 含有电解质的液体和液体粒子通过孔的通道引起孔的电阻的变化，从而产生由信号检测电路检测的信号。 选择电路元件的参数以提供电路关系，其使颗粒生成的信号与孔的直径无关。 这是通过利用具有低输出阻抗的电源和对于直流和直流A.D.都具有低输入阻抗的信号检测电路，即在感测到的信号的信号频率来实现的。 电路还可以包括电导率监测电路，其可利用一个或多个感测电极和/或附加电极来监测电解质的导电性变化，并将这些导电性变化与由信号感测的颗粒产生的信号相关联 - 检测电路以改变这些信号，以使它们独立于电解质电阻率。

公开(公告)号：US3944797A

公开(公告)日：1976-03-16

申请号：US495230

申请日：1974-08-06

申请人： Wallace H. Coulter ,  Walter R. Hogg

发明人： Wallace H. Coulter ,  Walter R. Hogg

IPC分类号： G01N15/10 ,  G01N15/02 ,  G06G7/12

CPC分类号： G01N15/0205

摘要： An apparatus for determining the correct particle sizes at predetermined percentiles of the size distribution of a particulate system having a known size distribution characteristic such as for example a normal or log-normal size distribution, wherein a portion of the particles are too small to be measured. The particulate system is first passed through a particle detecting device which can be of the Coulter type. The particle detecting device produces particle pulses proportional to the size of the particles in the particulate system which can be measured. At least three percentile size determining circuits receive the particle pulses and develop first, second and third particle size signals respectively, indicating the size of the particles in the particulate system at the first, second and third predetermined percentiles. Two of the size signals are combined in a particular manner based on the known size distribution characteristic for the type of particulate system to yield a combined signal which approximates or is and estimate of the third percentile signal. The combined signal is compared with the measured third signal to obtain an error signal. The error signal is used in turn to add into all three size percentile circuits compensation or correction signals for the total volume of particules too small to be included in the measurements. This process causes the error signal to decrease toward zero, and results in all three circuits having outputs truly representative of their nominal percentiles.

摘要翻译： 用于确定具有已知尺寸分布特性例如正常或对数正态尺寸分布的颗粒系统的尺寸分布的预定百分位数的正确粒度的装置，其中一部分颗粒太小而不能测量 。 颗粒系统首先通过可以是库尔特型的颗粒检测装置。 粒子检测装置产生与可以测量的颗粒系统中的颗粒尺寸成比例的粒子脉冲。 至少三个百分位数确定电路分别接收粒子脉冲并分别显示出第一，第二和第三粒度信号，表明在第一，第二和第三预定百分位数处的颗粒系统中颗粒的大小。 两个尺寸信号基于已知的颗粒系统类型的尺寸分布特性以特定的方式组合，以产生近似或估计第三百分位数信号的组合信号。 将组合信号与测量的第三信号进行比较以获得误差信号。 误差信号依次用于将所有三个百分位数的电路补偿补偿或校正信号，使得总体积的微粒太小而不能包含在测量中。 该过程导致误差信号向零降低，并且导致所有三个电路具有真正代表其标称百分位数的输出。

公开(公告)号：US5576185A

公开(公告)日：1996-11-19

申请号：US228791

申请日：1994-04-15

申请人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

发明人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

IPC分类号： B01D21/00 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/574

CPC分类号： G01N33/54333 ,  G01N33/553 ,  G01N33/56972 ,  G01N33/574 ,  Y10S436/824

摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including the non-selected population is separated from the particles with the selected population bound thereto. The particles can be heated for sterilization and endotoxin removal.

摘要翻译： 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。 颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。 允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含未选择群体的上清液与所选择的群体结合的颗粒分离。 可以加热颗粒进行灭菌和去除内毒素。

公开(公告)号：US4775833A

公开(公告)日：1988-10-04

申请号：US915185

申请日：1986-10-03

申请人： Ermi Roos ,  Wallace H. Coulter

发明人： Ermi Roos ,  Wallace H. Coulter

IPC分类号： G01N15/12 ,  G01N27/02 ,  G01N27/00

CPC分类号： G01N15/1227 ,  G01N2015/1281

摘要： A debris detector for a particle counter, such as a blood cell counter, of the COULTER (R) type. When a particle, or transient debris, passes through the sensing orifice of the particle detector, a voltage pulse occurs; whereas, when debris lodges in or against the orifice of the particle detector, a d.c. voltage shift occurs. The voltage at the orifice is capacitively coupled to an amplifier and the output of the amplifier is integrated. The capacitor coupling the orifice voltage to the amplifier causes an undershoot voltage at the trailing edge of each particle or transient debris pulse, such that a zero average voltage is applied to and, hence, from the amplifier for each voltage pulse. The integrated voltage is applied to a voltage comparator to provide an output signal therefrom whenever the integrated voltage exceeds a reference voltage. The comparator output signal is provided to a delay circuit having a delay related to the time required for a particle to pass through the COULTER detector orifice. If the comparator signal remains after the delay time, a debris alarm signal is provided. This occurs only if a d.c. voltage shift had occurred, since there is no trailing edge, and hence no undershoot to return the integrated voltage to zero prior to the delay time.

摘要翻译： 用于粒子计数器的碎片检测器，例如COULTER类型的血细胞计数器。 当颗粒或瞬态碎片通过颗粒检测器的感应孔时，会发生电压脉冲; 而当碎屑落入或抵靠颗粒检测器孔口时， 发生电压偏移。 孔口处的电压电容耦合到放大器，并且放大器的输出被集成。 将孔口电压耦合到放大器的电容器在每个粒子的后沿引起下冲电压或瞬态碎片脉冲，使得零平均电压被施加到每个电压脉冲的放大器，并且因此对于每个电压脉冲施加于放大器。 当积分电压超过参考电压时，积分电压被施加到电压比较器以提供输出信号。 比较器输出信号被提供给具有与颗粒通过COULTER检测器孔所需的时间有关的延迟的延迟电路。 如果比较器信号保持在延迟时间之后，则提供碎片报警信号。 这种情况只有在d.c. 发生电压偏移，因为没有后沿，因此在延迟时间之前没有下冲将积分电压返回到零。

公开(公告)号：US4534465A

公开(公告)日：1985-08-13

申请号：US541602

申请日：1983-10-13

申请人： William F. Rothermel ,  John P. Matthews ,  James W. Walker ,  Wallace H. Coulter

发明人： William F. Rothermel ,  John P. Matthews ,  James W. Walker ,  Wallace H. Coulter

IPC分类号： G01N33/48 ,  A47B73/00 ,  B01L9/06 ,  B65D81/30

CPC分类号： B01L9/06

摘要： A stackable cassette or rack for supporting a plurality of sealed sample containers having different diameters and/or lengths and transporting the same to a testing station of a hematology analyzer device. The cassette comprises a body having a top and a bottom and a rear portion, and the body includes a base, front and intermediate walls and a biasing means, connected between the front and intermediate walls, both walls longitudinally extend across the base and each wall has a plurality of equidistantly spaced openings therein arranged in a row lengthwise of the cassette which openings extend from the base to the top of the body. Each of the openings in the front wall has an upper edge and the spaced apart holes of both walls are in alignment. The biasing means are connected to the bottom of the body to provide secure and proper positioning for all sample containers inserted through the spaced openings in the front and intermediate walls and so that their upper walls abut against the upper edges of the openings in the front wall, the later to facilitate accurate optical reading of information placed on their upper wall surfaces. The biasing means also maintains a parallel orientation between the longitudinal axis' of the inserted sample containers and their receptacles. Further included is a plurality of equidistantly spaced channels arranged in a row lengthwise of the cassette and extending from the base to the top of the body and from the intermediate wall to the end of the rear portion of the body and open ended thereat and arranged so as to permit entry of a rod member.

摘要翻译： 一种用于支撑具有不同直径和/或长度的多个密封样品容器的可堆叠盒或支架，并将其运送到血液分析装置的检测站。 盒体包括具有顶部和底部以及后部的本体，并且主体包括基部，前壁和中间壁以及连接在前壁和中间壁之间的偏置装置，两个壁纵向延伸穿过底座和每个壁 具有多个等间隔开的开口，其中，所述开口沿着所述盒的纵向方向布置，所述开口从所述基部延伸到所述主体的顶部。 前壁中的每个开口具有上边缘，并且两个壁的间隔开的孔对准。 偏压装置连接到主体的底部，以便为穿过前壁和中间壁中的间隔开的开口插入的所有样品容器提供牢固且适当的定位，并使它们的上壁抵靠在前壁中的开口的上边缘 ，稍后便于准确地读取放置在其上壁表面上的信息。 偏置装置还保持插入的样本容器的纵向轴线与其插座之间的平行取向。 还包括多个等距间隔的通道，这些通道沿着盒的长度方向布置并且从基座延伸到主体的顶部并且从中间壁延伸到身体的后部的端部并且在其上开放并且布置成 以允许杆构件的进入。

公开(公告)号：US4298836A

公开(公告)日：1981-11-03

申请号：US96945

申请日：1979-11-23

申请人： Michael R. Groves ,  Wallace H. Coulter

发明人： Michael R. Groves ,  Wallace H. Coulter

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N27/00

CPC分类号： G01N15/12 ,  G01N2015/0073 ,  G01N2015/1037 ,  G01N2015/105 ,  G01N2015/1093 ,  G01N2015/1413

摘要： Apparatus and method wherein particles in a liquid stream are hydrodynamically focused to pass through an impedance sensing orifice, a low frequency current source provides a current through the orifice to produce a signal representative of the particle's size, a high frequency source provides a current through the orifice to produce a signal representative of the particle's size and internal resistance, a detector determines the particle's length, and a digital computer correlates the signals for each particle and calculates its shape factor, degree of deformation or natural shape, true volume and internal resistivity.

摘要翻译： 液体流中的颗粒被流体动力聚焦以通过阻抗感测孔的装置和方法，低频电流源提供通过孔的电流以产生表示颗粒尺寸的信号，高频源提供通过 孔以产生表示颗粒尺寸和内阻的信号，检测器确定颗粒的长度，并且数字计算机将每个颗粒的信号相关，并计算其形状因子，变形程度或自然形状，真实体积和内部电阻率。

公开(公告)号：US4281924A

公开(公告)日：1981-08-04

申请号：US6011

申请日：1979-01-24

申请人： Robert E. Auer ,  Wallace H. Coulter

发明人： Robert E. Auer ,  Wallace H. Coulter

IPC分类号： G01N15/14 ,  G01N21/49 ,  G01N21/64

CPC分类号： G01N15/1436 ,  G01N15/1459

摘要： An apparatus and method for illuminating particles, wherein a source of illumination provides a beam of illuminating radiation which perpendicularly intersects a stream of liquid having the particles suspended therein. The illuminator apparatus comprises a concave reflector surface having a center of curvature and an optical axis which is disposed perpendicularly relative to the stream and with the beam being positioned thereon. The stream of liquid defines a cylindrical lens having a focus positioned at the center of curvature. In operation, the illuminating radiation illuminates the particles, is refracted by the stream so as to converge to a focus, passes from that focus to a concave reflector surface, and reflects from the concave reflector surface back to the focus, so as to impinge upon the stream for a second time, to further illuminate the particles.

摘要翻译： 一种用于照射颗粒的装置和方法，其中照明源提供与其中悬浮有颗粒的液体流垂直相交的照射辐射束。 照明器装置包括具有曲率中心的凹面反射器表面和相对于流垂直设置且光束位于其上的光轴。 液体流限定了具有定位在曲率中心的焦点的柱面透镜。 在操作中，照射辐射照射颗粒，被流折射以聚焦到焦点，从该焦点通过到凹面反射器表面，并从凹面反射器表面反射回焦点，从而撞击 该流第二次，进一步照亮粒子。

公开(公告)号：US3961249A

公开(公告)日：1976-06-01

申请号：US597081

申请日：1975-07-18

申请人： Wallace H. Coulter

发明人： Wallace H. Coulter

IPC分类号： G01N15/12 ,  H03K5/153 ,  G01N27/00

CPC分类号： H03K5/153 ,  G01N15/1227

摘要： A particle analyzer for obtaining a narrow distribution of particles suspended in a liquid wherein electric pulses are produced by moving the liquid through a sensing zone. The analyzer includes elements for differentiating the pulses, short circuiting to ground pulse portions of one state and measuring the heights of pulse portions of another state as indications of particle sizes.

摘要翻译： 一种用于获得悬浮在液体中的微粒分布的粒子分析器，其中通过使液体通过感测区域产生电脉冲。 该分析器包括用于区分脉冲的元件，与一个状态的接地脉冲部分的短路，并测量另一个状态的脉冲部分的高度作为粒子的指示。