公开(公告)号：US4325909A

公开(公告)日：1982-04-20

申请号：US200143

申请日：1980-10-24

申请人： Wallace H. Coulter ,  Walter M. Mena

发明人： Wallace H. Coulter ,  Walter M. Mena

IPC分类号： G01N35/10 ,  G01N1/14 ,  G01N35/06

CPC分类号： G01N35/1011 ,  G01N2035/1025 ,  G01N2035/1086

摘要： A vertically and horizontally movable arm structure for fluid transfer is provided with a tubular member for linear oscillation of a holder for a fluid probe; the tubular member can additionally form a conduit for lead wire connected to the fluid probe. Sensors for the vertical position of the arm and the conductor leads from the sensors can be separately mounted and routed so that the sensor leads do not kink or interfere with the motion of the arm.

摘要翻译： 用于流体传送的垂直和水平可移动的臂结构设置有用于流体探针的保持器的线性振荡的管状构件; 管状构件可另外形成连接到流体探针的引线的导管。 传感器的臂和导体引线的垂直位置的传感器可以单独安装和布线，以便传感器引线不会扭曲或干扰臂的运动。

公开(公告)号：US5653686A

公开(公告)日：1997-08-05

申请号：US372196

申请日：1995-01-13

申请人： Wallace H. Coulter ,  Charles R. Shambaugh

发明人： Wallace H. Coulter ,  Charles R. Shambaugh

IPC分类号： A61J1/00 ,  A61J1/20 ,  B01L3/14 ,  G01N1/00 ,  A61M31/00

CPC分类号： G01N35/1079 ,  A61J1/2089 ,  B01L3/5082 ,  A61J1/201 ,  A61J1/2017 ,  A61J1/2058 ,  A61J1/2072 ,  A61J1/2075 ,  A61J1/2082 ,  A61J1/2086 ,  A61J1/2096

摘要： A method and transfer device for transferring fluids between closed vials. The transfer device includes a cylinder having a pair of opposing open tubes or tubular portions into which a pair of vials can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle and a vent needle which first is inserted into a first vial to vent the first vial into a vent chamber or reservoir formed in the transfer device before the transfer needle is inserted into the first vial. The first vial, stopper first, is inserted into first tube until seated onto an exposed end of the transfer needle. A second vial, stopper first, then is inserted into the second tube until seated onto the opposite exposed end of the transfer needle. A pressure differential between the closed vials preferably can be utilized to transfer a desired fluid portion between the vials.

摘要翻译： 一种用于在密封小瓶之间传送流体的方法和传送装置。 转移装置包括具有一对相对的开放管或管状部分的圆柱体，一对小瓶可插入其中以在其间传送流体。 管被壁分开，通过该壁将流体输送针和通气针延伸，首先将其插入第一小瓶中，以将第一小瓶排出到形成在转移装置中的通气室或储存器中，然后将输送针插入 第一个小瓶。 首先将第一个小瓶，塞子插入第一个管子，直到坐到转移针的暴露端上。 首先将第二个小瓶，塞子插入第二个管子中，直到安置在转移针的相对的暴露端上。 密封小瓶之间的压力差优选地可用于在小瓶之间转移期望的流体部分。

公开(公告)号：US3968429A

公开(公告)日：1976-07-06

申请号：US594209

申请日：1975-07-09

申请人： Wallace H. Coulter ,  Walter R. Hogg

发明人： Wallace H. Coulter ,  Walter R. Hogg

IPC分类号： G01N15/12 ,  G01N27/00

CPC分类号： G01N15/1245 ,  G01N15/1227

摘要： A particle analyzer of the Coulter type has at least first and second sensing zone arrangements, each having substantially the same dimensions and each generating a train of particle pulses in response to passage through the respective sensing zone of a sample containing a plurality of particles to be counted. A summing circuit connected to the output of each sensing zone sums together each of the trains of particle pulses to develop a summed train of pulses. Additional circuitry is connected to each sensing zone and the summing circuitry. This circuitry is operative to change mathematically at least the number of particle pulses in the summed train of particle pulses. The mathematically changed summed train of pulses and the train of pulses from the first and second sensing zones then are accumulated to produce an error corrected particle pulse count.

摘要翻译： 库尔特型的粒子分析仪具有至少第一和第二感测区域布置，每个具有基本上相同的尺寸，并且每个产生一系列粒子脉冲以响应于通过包含多个颗粒的样品的相应感测区域而成 算了 连接到每个感测区域的输出的求和电路将每个粒子脉冲列组合在一起，以产生一个总和的脉冲序列。 附加电路连接到每个感测区域和求和电路。 该电路用于在数学上改变粒子脉冲的总和列中的至少粒子脉冲的数量。 然后累积数学改变的脉冲序列和来自第一和第二感测区域的脉冲串，以产生误差校正的粒子脉冲计数。

公开(公告)号：US06900029B1

公开(公告)日：2005-05-31

申请号：US08556667

申请日：1995-11-13

申请人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

发明人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

IPC分类号： B01D21/00 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/574 ,  A01N1/02 ,  C12Q1/18 ,  G01N33/53

CPC分类号： G01N33/54333 ,  G01N33/553 ,  G01N33/56972 ,  G01N33/574 ,  Y10S436/824

摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernatant can be further enriched by multiple removal steps.

摘要翻译： 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。 颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。 然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。 通过多次去除步骤，可以进一步富集生物样品上清液中的富集群体。

公开(公告)号：US5238812A

公开(公告)日：1993-08-24

申请号：US517309

申请日：1990-05-01

申请人： Wallace H. Coulter ,  John D. Hollinger ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

发明人： Wallace H. Coulter ,  John D. Hollinger ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

IPC分类号： G01N33/50 ,  G01N33/569

CPC分类号： G01N33/5005 ,  G01N33/56966 ,  G01N33/56972 ,  Y10S436/808

摘要： A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

摘要翻译： 一种用于加速至少一种确定性生物反应的方法和装置，包括增加由其制备的测定精度。 该反应涉及选择的活体生物细胞，它们以小的样品体积制备，并且与具有抗体特异性至少与结合于其上的特定细胞的微球快速混合。 微球可以是磁性的，并且结合的细胞可以被磁性去除以分析剩余的血细胞群体。 微球可以顺序或同时引入。

公开(公告)号：US5223398A

公开(公告)日：1993-06-29

申请号：US587646

申请日：1990-09-20

申请人： Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos Rodriguez ,  Thomas Russell ,  Ronald Paul

发明人： Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos Rodriguez ,  Thomas Russell ,  Ronald Paul

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N35/00

CPC分类号： G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N15/12 ,  G01N15/14 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N35/0098 ,  Y10S436/808

摘要： A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological sample containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis.The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

摘要翻译： 一种用于自动并快速地检索，计数和/或分析全血样本或其部分的至少一个所选择的白细胞群体和/或其子集的方法和装置。 制备含有白细胞的体积的生物样品，并将至少一种特异性或优先选择的至少一些选定的生物细胞引入其中并在短时间内快速混合。 可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析以获得所需的白细胞群体分析。 生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特定细胞结合，或与结合抗体的溶血和微球体的组合。 微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。

公开(公告)号：US3936740A

公开(公告)日：1976-02-03

申请号：US442110

申请日：1974-02-13

申请人： Walter R. Hogg ,  Wallace H. Coulter

发明人： Walter R. Hogg ,  Wallace H. Coulter

IPC分类号： H03K5/14 ,  G01N27/00 ,  H03K5/20

CPC分类号： H03K5/14

摘要： An apparatus which includes circuitry for automatically sampling each pulse in a series of pulses a predetermined average number of times, sequentially storing the samples for a period of time, and reproducing the pulses from the samples, delayed in time.A method of operation is encompassed within the invention as well as use of the above apparatus in a particle study device.

摘要翻译： 一种装置，其包括用于以预定的平均次数自动采样一系列脉冲中的每个脉冲的电路，顺序地将样本存储一段时间，并且在时间上延迟地从样本中再现脉冲。

公开(公告)号：US5468616A

公开(公告)日：1995-11-21

申请号：US58692

申请日：1993-04-29

申请人： Wallace H. Coulter ,  John D. Hollinger ,  Kenneth H. Kortright ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

发明人： Wallace H. Coulter ,  John D. Hollinger ,  Kenneth H. Kortright ,  Thomas Russell ,  Carlos Rodriguez ,  Ronald Paul

IPC分类号： G01N33/50 ,  G01N33/569 ,  C12Q1/24 ,  G01N33/543

CPC分类号： G01N33/5005 ,  G01N33/56966 ,  G01N33/56972 ,  Y10S436/808

摘要： A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

摘要翻译： 一种用于加速至少一种确定性生物反应的方法和装置，包括增加由其制备的测定精度。 该反应涉及选择的活体生物细胞，它们以小的样品体积制备，并且与具有抗体特异性至少与结合于其上的特定细胞的微球快速混合。 微球可以是磁性的，并且结合的细胞可以被磁性去除以分析剩余的血细胞群体。 微球可以顺序或同时引入。

公开(公告)号：US5348859A

公开(公告)日：1994-09-20

申请号：US703163

申请日：1991-05-20

申请人： Robert F. Brunhouse ,  Constance M. Hajek ,  Thomas Russell ,  Wallace H. Coulter

发明人： Robert F. Brunhouse ,  Constance M. Hajek ,  Thomas Russell ,  Wallace H. Coulter

IPC分类号： G01N15/14 ,  G01N1/31 ,  G01N15/00 ,  G01N15/06 ,  G01N33/49 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  G01N33/53 ,  G01N33/48 ,  G06M11/02

CPC分类号： G01N1/312 ,  G01N15/147 ,  G01N33/54313 ,  G01N33/56972 ,  G01N33/574 ,  G01N15/0625 ,  G01N15/1475 ,  G01N2015/008 ,  G01N2015/1472 ,  G01N2015/1486

摘要： An optical screening method and apparatus for identifying and counting cells expressing selected characteristics or properties. The cells are combined with one or more different sets of microspheres, each set having a reactant bound thereto which will bind to a specific molecule which can exist on one or more types of the cells. The cells and microspheres are formed in a known volume on a slide and optically viewed to identify and count the type of cells to which the different sets of microspheres do or do not bind. The cell count then is related to the known volume to provide an absolute cell count. A plurality of different sample portions can be utilized with different reactants to obtain a multipart WBC differential/absolute count. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof.

摘要翻译： 用于鉴定和计数表达所选特征或性质的细胞的光学筛选方法和装置。 细胞与一个或多个不同组的微球组合，每组具有与其结合的反应物，其结合可存在于一种或多种类型细胞上的特定分子。 细胞和微球体在载玻片上的已知体积中形成，并且在光学上被观察以鉴定和计数不同组的微球体与其结合的细胞的类型。 然后，细胞计数与已知体积相关，以提供绝对细胞计数。 可以将多个不同的样品部分用于不同的反应物以获得多部分WBC差异/绝对计数。 通过具有不同的光学特性，例如尺寸，形状，颜色或其组合，不同组的微球被光学分化。

公开(公告)号：US5231005A

公开(公告)日：1993-07-27

申请号：US849481

申请日：1992-03-10

申请人： Thomas Russell ,  Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Ronald Paul ,  Constance M. Hajek ,  James C. Hudson

发明人： Thomas Russell ,  Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Ronald Paul ,  Constance M. Hajek ,  James C. Hudson

IPC分类号： G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N35/00

CPC分类号： G01N15/12 ,  G01N33/5002 ,  G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N15/14 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N35/0098 ,  Y10T436/101666 ,  Y10T436/117497 ,  Y10T436/24

摘要： A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

摘要翻译： 一种用于自动并快速地检索，计数和/或分析全血样本或其部分的至少一个选定细胞群体或形成体，例如白细胞群体及其至少一个子集的方法和装置。 制备含有白细胞的体积的生物培养基，并将至少一种至少一种选择的生物细胞特异性或优先选择的反应物引入其中并在短时间内快速混合。 可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析，以获得所需的白细胞群体和子集分析。 生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特定细胞结合，或与结合抗体的溶血和微球体的组合。 微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。